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[175682_at]

Description g2353799 /REP_DB=GenBank Identifier /GB=AF020555.1 /PROTEIN_GB=AAB68848.1 /CHR=4 /FEA=Mapped Transcript /DEF=lin-22
Cluster 6grp_total > 02      4grp_total > 01      6grp_cgc > 43      6grp_cgc_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 5.2
(-3)
A 		5.9
(-3)
A 		7.8
(-2.9)
A 		130.8
(3)
A 		126
B 32.7
(3)
A 		10.7
(-3)
A 		29.7
(2.2)
A 		12.7
(-2.5)
A 		22
C 7.6
(-3)
A 		6.9
(-3)
A 		33.3
(-1.6)
A 		117.9
(3)
A 		111
avr 15.2
(-2.4) 		7.8
(-3) 		23.6
(-1.8) 		87.1
(3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 13.9
(3)
A 		3.4
(-2.6)
A 		4.9
(-1.8)
A 		4.6
(-1.9)
A 		4.5
(-2)
A 		2.6
(-3)
A 		11
B 4.7
(1.5)
A 		5.5
(3)
A 		2.7
(-2.3)
A 		3.8
(-0.2)
A 		3.3
(-1.1)
A 		2.3
(-3)
A 		3
C 20.6
(-1.4)
A 		69
(3)
A 		3.9
(-3)
A 		8.8
(-2.5)
A 		3.7
(-3)
A 		4.3
(-2.9)
A 		65
avr 13.1
(-0.4) 		26
(3) 		3.8
(-2.8) 		5.7
(-2.3) 		3.8
(-2.8) 		3.1
(-3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 1.9
(-2.8)
A 		1.3
(-3)
A 		2
(-2.8)
A 		19.1
(3)
A 		18
B 10.4
(3)
A 		2.6
(-3)
A 		6.9
(0.3)
A 		2.7
(-2.9)
A 		8
C 2.5
(-2.8)
A 		2
(-3)
A 		11.6
(0.2)
A 		19.9
(3)
A 		18
avr 4.9
(-1.5) 		2
(-3) 		6.8
(-0.6) 		13.9
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 2.6
(3)
A 		1.2
(-1.2)
A 		1.1
(-1.5)
A 		1.2
(-1.2)
A 		1.3
(-0.9)
A 		0.6
(-3)
A 		2
B 1.6
(1.9)
A 		1.8
(3)
A 		0.7
(-3)
A 		1
(-1.4)
A 		1.3
(0.3)
A 		0.9
(-1.9)
A 		1
C 7.6
(-1)
A 		21
(3)
A 		1
(-3)
A 		1.8
(-2.8)
A 		1.2
(-2.9)
A 		1.4
(-2.9)
A 		20
avr 3.9
(-0.5) 		8
(3) 		0.9
(-3) 		1.3
(-2.7) 		1.3
(-2.7) 		1
(-2.9) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175682_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19881
Sequence Type Exemplar sequence
Representative Public ID 2353799 NCBI
Target Description g2353799 /REP_DB=GenBank Identifier /GB=AF020555.1 /PROTEIN_GB=AAB68848.1 /CHR=4 /FEA=Mapped Transcript /DEF=lin-22
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067880
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067880 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-22) (lin-22) mRNA, complete cds. None
SNAP00000036523 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3014874:3028209:1 None
Y54G2A.1 ENSEMBL cdna:known chromosome:CEL140:IV:3019737:3022212:1 gene:Y54G2A.1 None
GENEFINDER00000036580 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3019737:3022501:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3019740-3022358(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-22
Ensembl Y54G2A.1
Entrez Gene 177082 Entrez gene
SwissProt O16853 EMBL-EBI
WormBase CE26703 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68522 Abnormal cell lineage protein 22 [Caenorhabditis elegans] ref|NP_500281.1| abnormal cell LINeage family member (lin-22) [Caenorhabditis elegans] gb|AAB68848.1| lin-22 [Caenorhabditis elegans] 2.0E-71
blast AAK68522 Abnormal cell lineage protein 22 [Caenorhabditis elegans] ref|NP_500281.1| abnormal cell LINeage family member (lin-22) [Caenorhabditis elegans] gb|AAB68848.1| lin-22 [Caenorhabditis elegans] 4.0E-70
blast AAK68522 Abnormal cell lineage protein 22 [Caenorhabditis elegans] ref|NP_500281.1| abnormal cell LINeage family member (lin-22) [Caenorhabditis elegans] gb|AAB68848.1| lin-22 [Caenorhabditis elegans] 5.0E-39
blast CAE59881 Hypothetical protein CBG03361 [Caenorhabditis briggsae] 1.0E-30
blast AAH39152 HES1 protein [Homo sapiens] 9.0E-12
blast AAH39152 HES1 protein [Homo sapiens] 1.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 6.2E-17
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 1.5E-6
Sequence
Target Sequence 
>C. ELEGANS:175682_AT
agaattccatccaacattccaaatgggaaaaagctgatattctcgaaatggctgtcgaat
acctccaacaactccgtagtgctcaaccatgctccttatcaccttcaacatcatccattt
caactccaccaactccaaaagaagaaattcgaaatattaaagtacctcttaatccaatag
cttcatttctgaacccaatgatgcagcaatacgtggcatatcagcagctggctcagttat
caatgtacactcaattatttaataatcccgctggagtaccactgagagcagatgcagggg
taactgctcagtcaccagagctggcagaaaagttaaaaattgaggatcgaagtagagtgt
agaatttggaattgttgtcaagagccaccagttttgatgtgaaattgataatctactagt
tagccacctaaggtctataaaatagcttcagaggcagggtttcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAATTCCATCCAACATTCCAAATG 34 77 175 Antisense
GAAATGGCTGTCGAATACCTCCAAC 216 361 219 Antisense
GTACCTCTTAATCCAATAGCTTCAT 77 459 336 Antisense
AATAGCTTCATTTCTGAACCCAATG 698 171 350 Antisense
TACGTGGCATATCAGCAGCTGGCTC 245 647 384 Antisense
GGCTCAGTTATCAATGTACACTCAA 469 539 404 Antisense
TAATCCCGCTGGAGTACCACTGAGA 224 635 437 Antisense
AACTGCTCAGTCACCAGAGCTGGCA 52 143 476 Antisense
ATTGTTGTCAAGAGCCACCAGTTTT 522 3 545 Antisense
GATAATCTACTAGTTAGCCACCTAA 542 421 581 Antisense
ATAGCTTCAGAGGCAGGGTTTCGGC 97 23 616 Antisense
Sequence Source GenBank