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[175679_at]

Description g4689162 /REP_DB=GenBank Identifier /GB=AF096618.1 /PROTEIN_GB=AAD27790.1 /CHR=2 /FEA=Mapped Transcript /DEF=EGL-27
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 47      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 57      6grp_total > 37      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 16      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2005
1 2 3 4 range
A 3737.6
(3)
P 		2340.6
(-1.7)
P 		1958.7
(-3)
P 		3363.4
(1.7)
P 		1779
B 3766.4
(3)
P 		2153.6
(-1.8)
P 		1761.5
(-3)
P 		3616.6
(2.6)
P 		2005
C 3444.4
(2.6)
P 		2085
(-3)
P 		2118.7
(-2.9)
P 		3549.9
(3)
P 		1465
avr 3649.5
(3) 		2193.1
(-2.1) 		1946.3
(-3) 		3510
(2.5) 		1703

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1652
1 2 3 4 5 6 range
A 3203.2
(3)
P 		2461.8
(0.3)
P 		1550.8
(-3)
P 		1781.7
(-2.2)
P 		1849.8
(-1.9)
P 		2723.1
(1.3)
P 		1652
B 2965.1
(2.7)
P 		2863.2
(2.3)
P 		1971.9
(-2.1)
P 		2147.9
(-1.2)
P 		1786.9
(-3)
P 		3016.7
(3)
P 		1230
C 2673.2
(3)
P 		2443.4
(1.6)
P 		1965.1
(-1.4)
P 		2262.6
(0.4)
P 		1714.6
(-3)
P 		2416.9
(1.4)
P 		959
avr 2947.2
(3) 		2589.5
(1.2) 		1829.3
(-2.8) 		2064.1
(-1.6) 		1783.8
(-3) 		2718.9
(1.8) 		1163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 953
1 2 3 4 range
A 1360.9
(3)
P 		508.1
(-2.9)
P 		512
(-2.9)
P 		492.3
(-3)
P 		869
B 1201.1
(3)
P 		531.9
(-2.1)
P 		408.2
(-3)
P 		770.7
(-0.3)
P 		793
C 1140.2
(3)
P 		599.4
(-3)
P 		738.4
(-1.5)
P 		598.6
(-3)
P 		542
avr 1234.1
(3) 		546.5
(-3) 		552.9
(-2.9) 		620.5
(-2.4) 		688

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 787
1 2 3 4 5 6 range
A 590.5
(-0.2)
P 		875.5
(3)
P 		337.7
(-3)
P 		451.9
(-1.7)
P 		558.2
(-0.5)
P 		663
(0.6)
P 		538
B 1031.7
(2.1)
P 		953
(1.4)
P 		478.8
(-3)
P 		568.9
(-2.2)
P 		726
(-0.7)
P 		1124.9
(3)
P 		646
C 990.8
(3)
P 		745.5
(0.3)
P 		500.2
(-2.5)
P 		455
(-3)
P 		554.8
(-1.9)
P 		809.1
(1)
P 		536
avr 871
(3) 		858
(2.8) 		438.9
(-3) 		491.9
(-2.3) 		613
(-0.6) 		865.7
(2.9) 		432
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175679_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19738
Sequence Type Exemplar sequence
Representative Public ID 4689162 NCBI
Target Description g4689162 /REP_DB=GenBank Identifier /GB=AF096618.1 /PROTEIN_GB=AAD27790.1 /CHR=2 /FEA=Mapped Transcript /DEF=EGL-27
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171011(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171011 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 A 
C04A2.3a ENSEMBL cdna:known chromosome:CEL140:II:6834951:6847836:1 gene:C04A2.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174121 3 1 NM_001026740 188531_s_at (11)
NM_171011 175679_at (11), 188531_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6834945-6847833(+) 93.65 100.0
Public Domain and Genome References
Ensembl C04A2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81126 Egg laying defective protein 27, isoform a [Caenorhabditis elegans] ref|NP_741012.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] sp|Q09228|EGL27_CAEEL Egg laying defective protein 0.0
blast AAD27790 EGL-27 [Caenorhabditis elegans] 0.0
blast AAM81127 Egg laying defective protein 27, isoform b [Caenorhabditis elegans] ref|NP_001021911.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Sequence
Target Sequence 
>C. ELEGANS:175679_AT
gcgtcctcaacatcagtaaacaccgttgccctctcacaaacccatctcaaacaatttgtc
ccggttttcggtcttatttatccttgtttcactgtgaaccttttttttaattctctgcta
atgtgatccttctgggtatcatattttaagttgtattgtaaatacgttttcattatatca
ttatccccatcatcatcatcatctcttacgtgcatttcgttcaattctgttaaaagtttt
ttggcatctagaagtgtctcgttgttatccgcttttctccccttttcccttcccttctaa
atctcttctcattcacaaacctcgatcagacttggcaccggtatatttttacatgatttg
ttatgttcgttaacctcatctttctatcagcccctgcttcttttgaatttacaatttgta
tctctctcattcccttcagccaatttatatccctattcaactcgtttctctcagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGTCCTCAACATCAGTAAACACCG 689 289 3444 Antisense
ACCCATCTCAAACAATTTGTCCCGG 63 91 3483 Antisense
TCGGTCTTATTTATCCTTGTTTCAC 168 607 3511 Antisense
TTCTCTGCTAATGTGATCCTTCTGG 142 693 3554 Antisense
TCATTATATCATTATCCCCATCATC 486 619 3613 Antisense
CATCATCTCTTACGTGCATTTCGTT 539 213 3640 Antisense
ACGTGCATTTCGTTCAATTCTGTTA 373 93 3651 Antisense
TGGCATCTAGAAGTGTCTCGTTGTT 147 547 3685 Antisense
TTCGTTAACCTCATCTTTCTATCAG 666 693 3809 Antisense
TCATTCCCTTCAGCCAATTTATATC 468 619 3870 Antisense
CCTATTCAACTCGTTTCTCTCAGGA 213 269 3895 Antisense
Sequence Source GenBank