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[175678_at]

Description g2623942 /REP_DB=GenBank Identifier /GB=AF032112.1 /PROTEIN_GB=AAC47803.1 /CHR=1 /FEA=Mapped Transcript /DEF=DAF-16
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 83      6grp_total > 49      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1177
1 2 3 4 range
A 409.5
(-1.6)
P 		197.2
(-2.7)
P 		133.3
(-3)
P 		1309.8
(3)
P 		1177
B 303
(-2.2)
P 		189.2
(-3)
P 		243
(-2.6)
P 		1022.7
(3)
P 		834
C 338.8
(-2.3)
P 		256.2
(-2.7)
P 		207.7
(-3)
P 		1263.9
(3)
P 		1056
avr 350.4
(-2.1) 		214.2
(-2.9) 		194.7
(-3) 		1198.8
(3) 		1004

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 594
1 2 3 4 5 6 range
A 484.8
(0.1)
P 		349.5
(-1.4)
P 		205
(-3)
P 		378.5
(-1.1)
P 		453.3
(-0.2)
P 		742.3
(3)
P 		537
B 521.7
(2.9)
P 		298.5
(-0.6)
P 		148.8
(-3)
P 		417.5
(1.3)
P 		406.5
(1.1)
P 		526.8
(3)
P 		378
C 630.5
(3)
P 		289.3
(-3)
P 		413.8
(-0.8)
P 		325.4
(-2.4)
P 		588.6
(2.3)
P 		484.2
(0.4)
P 		341
avr 545.7
(2.3) 		312.4
(-2) 		255.9
(-3) 		373.8
(-0.8) 		482.8
(1.1) 		584.4
(3) 		329
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 149.1
(1.4)
P 		42.8
(-2.7)
P 		34.8
(-3)
P 		191.7
(3)
P 		157
B 96.6
(-1.3)
P 		46.7
(-3)
P 		56.3
(-2.7)
P 		217.9
(3)
P 		171
C 112.2
(-1.3)
P 		73.6
(-2.9)
P 		72.4
(-3)
P 		213.1
(3)
P 		141
avr 119.3
(-0.5) 		54.4
(-3) 		54.5
(-3) 		207.6
(3) 		153

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 89.4
(-1)
P 		124.3
(0.5)
P 		44.6
(-3)
P 		96
(-0.7)
P 		136.8
(1.1)
P 		180.7
(3)
P 		136
B 181.5
(2.4)
P 		99.3
(-0.6)
P 		36.1
(-3)
P 		110.6
(-0.2)
P 		165.1
(1.8)
P 		196.4
(3)
P 		160
C 233.7
(3)
P 		88.3
(-2.2)
P 		105.3
(-1.6)
P 		65.4
(-3)
P 		190.4
(1.5)
P 		162.1
(0.4)
P 		168
avr 168.2
(2.4) 		104
(-0.9) 		62
(-3) 		90.7
(-1.5) 		164.1
(2.2) 		179.7
(3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175678_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19688
Sequence Type Exemplar sequence
Representative Public ID 2623942 NCBI
Target Description g2623942 /REP_DB=GenBank Identifier /GB=AF032112.1 /PROTEIN_GB=AAC47803.1 /CHR=1 /FEA=Mapped Transcript /DEF=DAF-16
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026427(11), NM_001026424(11), NM_001026423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026427 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026424 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026423 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
R13H8.1b ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784824:1 gene:R13H8.1 11/11 A 
R13H8.1c ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784821:1 gene:R13H8.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026422 5/11 Cross Hyb Matching Probes None
R13H8.1a 5/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172981 6 3 NM_001026422 175823_s_at (11)
NM_001026423 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026424 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026427 175678_at (11), 175823_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10770821-10784752(+) 96.64 100.0
Public Domain and Genome References
Ensembl R13H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021598 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021596 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021595 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82919.1| Abnormal dauer formation protein 16, isoform c [Caenorhabditis elegans] gb|AAC47803.1| DAF-16 [Caenorhabditis elegans] gb|AAB84390.1| fork head-related transcription factor DAF-16a1 [Caenorhabditis elegans] 0.0
blast NP_001021594 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82918.1| Abnormal dauer formation protein 16, isoform b [Caenorhabditis elegans] gb|AAB84391.1| fork head-related transcription factor DAF-16a2 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 8.968310171678829E-44
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 2.00385680398449E-43
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.4E-22
Pfam IPR001766 EMBL-EBI Fork head transcription factor 8.7E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 6.2E-23
Sequence
Target Sequence 
>C. ELEGANS:175678_AT
aaatctcagttttaaatggtgtggcaatatatcggatccccctctacaccagaacagtct
tgcaatttcagagaatgattttcagatttttcatatcacaggccccctttttttgcttgt
ttttttctctacctctctttcttttcattctatttctctctcttgttttctctctgttat
cctgtacattttccttccaattctttctggctatttctgattttcgagttcatattctct
acgtctcactttctctcgcgccacgccccctttttcgtctccctccgcccccaaatatat
ttgcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCTCAGTTTTAAATGGTGTGGC 308 115 2702 Antisense
GGTGTGGCAATATATCGGATCCCCC 256 499 2719 Antisense
CCTCTACACCAGAACAGTCTTGCAA 636 267 2742 Antisense
GAACAGTCTTGCAATTTCAGAGAAT 534 351 2753 Antisense
TCAGATTTTTCATATCACAGGCCCC 465 623 2783 Antisense
TTTTCTCTCTGTTATCCTGTACATT 432 677 2867 Antisense
TGTTATCCTGTACATTTTCCTTCCA 647 561 2876 Antisense
TCCAATTCTTTCTGGCTATTTCTGA 255 587 2897 Antisense
TTCTTTCTGGCTATTTCTGATTTTC 436 691 2902 Antisense
CGAGTTCATATTCTCTACGTCTCAC 176 251 2926 Antisense
CCTCCGCCCCCAAATATATTTGCGA 240 265 2983 Antisense
Sequence Source GenBank