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[175657_s_at]

Description g7963656 /REP_DB=GenBank Identifier /GB=AF229855.1 /PROTEIN_GB=AAF71303.1 /CHR=1 /FEA=Mapped Transcript /DEF=dual oxidase
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 81      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 53      6grp_total > 82      4grp_total > 60      6grp_highest_sub > 05      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 568
1 2 3 4 range
A 670.1
(1.8)
P 		539.1
(0.3)
P 		778.2
(3)
P 		247.3
(-3)
P 		531
B 642.8
(1)
P 		677.3
(1.5)
P 		789.4
(3)
P 		351.4
(-3)
P 		438
C 594.1
(0.6)
P 		716
(1.9)
P 		815.1
(3)
P 		266.6
(-3)
P 		549
avr 635.7
(1.1) 		644.1
(1.2) 		794.2
(3) 		288.4
(-3) 		506

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1070
1 2 3 4 5 6 range
A 552.1
(-2.1)
P 		748.6
(-1)
P 		698.7
(-1.3)
P 		1469.1
(3)
P 		1323.6
(2.2)
P 		398.8
(-3)
P 		1070
B 658.6
(-1.9)
P 		813.8
(-0.9)
P 		905.9
(-0.2)
P 		1391.7
(3)
P 		1294.5
(2.4)
P 		493.2
(-3)
P 		899
C 593.5
(-2.2)
P 		774.1
(-1)
P 		892.4
(-0.2)
P 		1313.5
(2.5)
P 		1382.7
(3)
P 		475
(-3)
P 		908
avr 601.4
(-2.1) 		778.8
(-0.9) 		832.3
(-0.6) 		1391.4
(3) 		1333.6
(2.6) 		455.7
(-3) 		936
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 248
1 2 3 4 range
A 244
(3)
P 		117
(-0.7)
P 		203.4
(1.8)
P 		36.2
(-3)
P 		208
B 205
(3)
P 		167.3
(1.3)
P 		182.9
(2)
P 		74.9
(-3)
P 		130
C 196.7
(0.8)
P 		205.8
(1)
P 		284.1
(3)
P 		44.9
(-3)
P 		239
avr 215.2
(2.7) 		163.4
(0.9) 		223.5
(3) 		52
(-3) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 429
1 2 3 4 5 6 range
A 101.8
(-2.9)
P 		266.2
(0.4)
P 		152.2
(-1.9)
P 		372.6
(2.5)
P 		399.4
(3)
P 		97.1
(-3)
P 		302
B 229.2
(-2.2)
P 		270.9
(-1.5)
P 		220
(-2.4)
P 		368.6
(0.2)
P 		526
(3)
P 		183.9
(-3)
P 		342
C 220
(-1.7)
P 		236.2
(-1.4)
P 		227.1
(-1.6)
P 		264.2
(-0.8)
P 		447.4
(3)
P 		159
(-3)
P 		288
avr 183.7
(-2.3) 		257.8
(-0.9) 		199.8
(-2) 		335.1
(0.6) 		457.6
(3) 		146.7
(-3) 		311
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175657_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19700
Sequence Type Exemplar sequence
Representative Public ID 7963656 NCBI
Target Description g7963656 /REP_DB=GenBank Identifier /GB=AF229855.1 /PROTEIN_GB=AAF71303.1 /CHR=1 /FEA=Mapped Transcript /DEF=dual oxidase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058285 NCBI Caenorhabditis elegans BLIstered cuticle family member (bli-3) (bli-3) mRNA, complete cds. 11/11 None
SNAP00000010366 ENSEMBL cdna:SNAP chromosome:CEL140:I:146623:149782:-1 11/11 None
GENEFINDER00000010373 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:146716:154819:-1 11/11 None
F56C11.1 ENSEMBL cdna:known chromosome:CEL140:I:146499:154819:-1 gene:F56C11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:146622-154819(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bli-3
Ensembl F56C11.1
Entrez Gene 171608 Entrez gene
SwissProt O61213 EMBL-EBI
WormBase CE28463 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17311_AT respiratory burst oxidase protein A (RbohA) / NADPH oxidase at
ATH1-121501:250629_AT respiratory burst oxidase protein A (RbohA) / NADPH oxidase at
DROSOPHILA_2:1636572_AT dm
DROSGENOME1:145670_AT dm
CHICKEN:GGAAFFX.1631.1.S1_S_AT similar to dual oxidase 2 precursor; dual oxidase-like domains 2; nicotinamide adenine dinucleotide phosphate oxidase; flavoprotein NADPH oxidase; NADPH thyroid oxidase 2; NADH/NADPH thyroid oxidase p138-tox; NADPH oxidase/peroxidase DUOX2 ... gga
HG-U133_PLUS_2:219727_AT dual oxidase 2 hs
HG-U133A:219727_AT dual oxidase 2 hs
HG-FOCUS:219727_AT dual oxidase 2 hs
HG-U133A_2:219727_AT dual oxidase 2 hs
U133_X3P:G7657434_3P_S_AT dual oxidase 2 hs
HG-U95D:79337_AT dual oxidase 2 hs
RAE230A:1387414_AT dual oxidase 2 rn
RAT230_2:1387414_AT dual oxidase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4601 peroxidase activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK73882 Blistered cuticle protein 3 [Caenorhabditis elegans] ref|NP_490686.3| BLIstered cuticle family member (bli-3) [Caenorhabditis elegans] 0.0
blast AAF71303 dual oxidase [Caenorhabditis elegans] 0.0
blast T32909 hypothetical protein F56C11.1 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.088
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.6E-64
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 3.6E-64
Pfam IPR002916 EMBL-EBI Ferric reductase-like transmembrane component 2.8E-61
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490686.3 7 590-612,988-1006,1026-1048,1069-1091,1129-1151,1158-1180,1190-1209
F56C11.1 7 590-612,988-1006,1026-1048,1069-1091,1129-1151,1158-1180,1190-1209
SNAP00000010366 6 125-143,163-185,206-228,266-288,295-317,327-346
GENEFINDER00000010373 7 590-612,988-1006,1026-1048,1069-1091,1129-1151,1158-1180,1190-1209
Sequence
Target Sequence 
>C. ELEGANS:175657_S_AT
gaatacaggatcgccatttccattaatccatatgaaaggaccatatggtgatggtaacca
agaatggatggattatgaagttgcaataatggttggagcaggaatcggagtgactccata
tgcatcgacacttgttgatcttgtacaacgaacatcaagtgactcatttcacagagttcg
ttgccgtaaagtatatttcctatgggtgtgctcaactcacaagaactatgaatggtttgt
ggatgtgctcaagaacgtggaagaccaagcaaggtcgggaattttggagacacatatctt
tgtcactcagacgttccacaagtttgatttgagaactactatgctttacatttgcgagaa
gcacttccgtgccaccaactcaggaatttcaatgtttactggtctccacgctaagaacca
tttcggacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATACAGGATCGCCATTTCCATTA 623 323 3903 Antisense
GATCGCCATTTCCATTAATCCATAT 487 417 3911 Antisense
ACTCCATATGCATCGACACTTGTTG 402 99 4015 Antisense
TGCATCGACACTTGTTGATCTTGTA 365 579 4023 Antisense
AACGAACATCAAGTGACTCATTTCA 531 145 4049 Antisense
TCATTTCACAGAGTTCGTTGCCGTA 375 619 4066 Antisense
GAGTTCGTTGCCGTAAAGTATATTT 504 395 4076 Antisense
GAGACACATATCTTTGTCACTCAGA 416 393 4189 Antisense
GTCACTCAGACGTTCCACAAGTTTG 607 465 4204 Antisense
ATGTTTACTGGTCTCCACGCTAAGA 530 43 4294 Antisense
TCCACGCTAAGAACCATTTCGGACG 230 589 4307 Antisense
Sequence Source GenBank