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[175644_s_at]

Description g7542552 /REP_DB=GenBank Identifier /GB=AF239998.1 /PROTEIN_GB=AAF63494.1 /CHR=5 /FEA=Mapped Transcript /DEF=MDF-1
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 97      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 81      6grp_total > 87      4grp_total > 15      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 266.5
(3)
P 		142
(-3)
P 		162
(-2)
P 		195.4
(-0.4)
P 		125
B 230
(3)
P 		140.8
(-3)
P 		143.9
(-2.8)
P 		220.2
(2.3)
P 		89
C 283.8
(3)
P 		134.3
(-3)
P 		239.3
(1.2)
P 		266.3
(2.3)
P 		150
avr 260.1
(3) 		139
(-3) 		181.7
(-0.9) 		227.3
(1.4) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 187.6
(-3)
P 		234.2
(-0.8)
P 		315.6
(3)
P 		279.4
(1.3)
P 		285.3
(1.6)
P 		285.1
(1.6)
P 		128
B 268.8
(-2)
P 		283.9
(-1.5)
P 		238.8
(-3)
P 		265.7
(-2.1)
P 		311.2
(-0.6)
P 		421.6
(3)
P 		183
C 258.4
(-1.9)
P 		261.9
(-1.7)
P 		240
(-3)
P 		257.3
(-2)
P 		307.1
(1)
P 		340.9
(3)
P 		101
avr 238.3
(-3) 		260
(-1.8) 		264.8
(-1.6) 		267.5
(-1.4) 		301.2
(0.4) 		349.2
(3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 97
(3)
P 		30.8
(-2.8)
P 		42.3
(-1.8)
P 		28.6
(-3)
P 		68
B 73.3
(3)
P 		34.8
(-2.8)
P 		33.4
(-3)
P 		46.9
(-1)
P 		40
C 93.9
(3)
P 		38.6
(-3)
P 		83.4
(1.9)
P 		44.9
(-2.3)
P 		55
avr 88.1
(3) 		34.7
(-3) 		53
(-0.9) 		40.1
(-2.4) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 34.6
(-3)
P 		83.3
(2.7)
P 		68.7
(1)
P 		70.9
(1.2)
P 		86.1
(3)
P 		69.4
(1.1)
P 		52
B 93.5
(-0.9)
P 		94.5
(-0.8)
P 		58
(-3)
P 		70.4
(-2.3)
P 		126.4
(1.1)
P 		157.2
(3)
P 		99
C 95.8
(1.2)
P 		79.9
(-0.3)
P 		61.1
(-2.1)
P 		51.8
(-3)
P 		99.4
(1.6)
P 		114.1
(3)
P 		62
avr 74.6
(-1.6) 		85.9
(-0.3) 		62.6
(-3) 		64.4
(-2.8) 		104
(1.9) 		113.6
(3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175644_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19910
Sequence Type Exemplar sequence
Representative Public ID 7542552 NCBI
Target Description g7542552 /REP_DB=GenBank Identifier /GB=AF239998.1 /PROTEIN_GB=AAF63494.1 /CHR=5 /FEA=Mapped Transcript /DEF=MDF-1
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073061(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073061 NCBI Caenorhabditis elegans MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-1) (mdf-1) mRNA, complete cds. 11/11 None
C50F4.11 ENSEMBL cdna:known chromosome:CEL140:V:9544367:9546810:-1 gene:C50F4.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009315 6/11 Cross Hyb Matching Probes None
GENEFINDER00000009321 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9544366-9546808(-) 98.96 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mdf-1
Ensembl C50F4.11
Entrez Gene 179338 Entrez gene
SwissProt Q9NGT4 EMBL-EBI
WormBase CE28212 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94745 Hypothetical protein C50F4.11 [Caenorhabditis elegans] ref|NP_505462.1| MAD (yeast Mitosis Arrest DeFicient) related family member (mdf-1) [Caenorhabditis elegans] gb|AAF63494.1| MDF-1 [Caenorhabditis elegans] 0.0
blast CAE72194 Hypothetical protein CBG19302 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008672 EMBL-EBI Mitotic checkpoint 1.1E-5
Sequence
Target Sequence 
>C. ELEGANS:175644_S_AT
ggcgaccgaaattcgattccggctttcttagcagcaatgactcttcagctggaacaggat
cgagattttgacgagacgatgcatgagagaacacatacattcagtgtcttacacgaggac
taaatcattgagctatctagatctcaattatttttcttcaaaggagaagctgttaaatac
actgttacctgaatatttgatgtttcattccattttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGACCGAAATTCGATTCCGGCTT 548 541 1930 Antisense
GATTCCGGCTTTCTTAGCAGCAATG 80 427 1944 Antisense
GCAATGACTCTTCAGCTGGAACAGG 664 321 1963 Antisense
GCTGGAACAGGATCGAGATTTTGAC 52 295 1977 Antisense
GACGAGACGATGCATGAGAGAACAC 578 379 1999 Antisense
GAACACATACATTCAGTGTCTTACA 561 351 2018 Antisense
GTGTCTTACACGAGGACTAAATCAT 75 485 2033 Antisense
GCTATCTAGATCTCAATTATTTTTC 584 303 2061 Antisense
AAGCTGTTAAATACACTGTTACCTG 597 147 2096 Antisense
TACACTGTTACCTGAATATTTGATG 161 643 2107 Antisense
ATTTGATGTTTCATTCCATTTTATT 452 17 2124 Antisense
Sequence Source GenBank