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[175633_at]

Description g2467303 /REP_DB=GenBank Identifier /GB=AB000918.1 /PROTEIN_GB=BAA22596.1 /CHR=3 /FEA=Mapped Transcript /DEF=VHA-2
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 20      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 07      6grp_total > 41      4grp_total > 10      6grp_highest_sub > 03_04      6grp_cgc > 71      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8354
1 2 3 4 range
A 15541.3
(-3)
P 		18058.6
(-0.6)
P 		17255.1
(-1.3)
P 		21710.3
(3)
P 		6169
B 17510.4
(-0.3)
P 		16365.3
(-3)
P 		17330
(-0.7)
P 		18904.8
(3)
P 		2540
C 13979.5
(-2.5)
P 		14245
(-2.3)
P 		13356.2
(-3)
P 		20615.6
(3)
P 		7259
avr 15677.1
(-3) 		16223
(-2.3) 		15980.4
(-2.6) 		20410.2
(3) 		4733

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7988
1 2 3 4 5 6 range
A 14766.8
(3)
P 		13752.2
(1.2)
P 		13899
(1.5)
P 		11830.6
(-2.1)
P 		11326.3
(-3)
P 		11411.6
(-2.9)
P 		3441
B 12707.9
(0.2)
P 		13731.5
(1.3)
P 		15405.4
(3)
P 		12117.8
(-0.4)
P 		10274.2
(-2.3)
P 		9633.2
(-3)
P 		5772
C 12339.5
(-1.4)
P 		13447.1
(-0.5)
P 		15027.2
(0.8)
P 		17621.2
(3)
P 		10951.1
(-2.6)
P 		10433.1
(-3)
P 		7188
avr 13271.4
(0.9) 		13643.6
(1.4) 		14777.2
(3) 		13856.5
(1.7) 		10850.5
(-2.5) 		10492.6
(-3) 		4285
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2481
1 2 3 4 range
A 5658.6
(3)
P 		3920
(-1.2)
P 		4510.8
(0.2)
P 		3178
(-3)
P 		2481
B 5583.9
(3)
P 		4041.9
(-2.9)
P 		4015.8
(-3)
P 		4028.6
(-3)
P 		1568
C 4627.6
(2.9)
P 		4095.5
(0.2)
P 		4655
(3)
P 		3476.1
(-3)
P 		1179
avr 5290
(3) 		4019.1
(-1.4) 		4393.9
(-0.1) 		3560.9
(-3) 		1729

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2169
1 2 3 4 5 6 range
A 2722.1
(-3)
P 		4891
(3)
P 		3026.9
(-2.2)
P 		3000.5
(-2.2)
P 		3417.8
(-1.1)
P 		2778.4
(-2.8)
P 		2169
B 4421.7
(2.3)
P 		4570.6
(3)
P 		3740.9
(-0.7)
P 		3209.6
(-3)
P 		4174.3
(1.3)
P 		3592.2
(-1.3)
P 		1361
C 4573.7
(3)
P 		4103.1
(0.4)
P 		3824.7
(-1.2)
P 		3543.6
(-2.7)
P 		3543.6
(-2.7)
P 		3492.6
(-3)
P 		1081
avr 3905.8
(0.1) 		4521.6
(3) 		3530.8
(-1.7) 		3251.2
(-3) 		3711.9
(-0.8) 		3287.7
(-2.8) 		1270
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175633_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19827
Sequence Type Exemplar sequence
Representative Public ID 2467303 NCBI
Target Description g2467303 /REP_DB=GenBank Identifier /GB=AB000918.1 /PROTEIN_GB=BAA22596.1 /CHR=3 /FEA=Mapped Transcript /DEF=VHA-2
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066765
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
R10E11.2.2 ENSEMBL cdna:known chromosome:CEL140:III:9782052:9783118:1 gene:R10E11.2 11/11 A 
R10E11.2.1 ENSEMBL cdna:known chromosome:CEL140:III:9782059:9783118:1 gene:R10E11.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_066765 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9782047-9783112(+) 96.28 100.0
Public Domain and Genome References
Ensembl R10E11.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA82355 Hypothetical protein R10E11.2 [Caenorhabditis elegans] ref|NP_499166.1| Vacuolar H ATPase family member (vha-2) [Caenorhabditis elegans] ref|NP_500188.1| Vacuolar H ATPase family member (vha-3) [Caenorhabditis elegans] gb|AAF59473.1| Vacuolar h atpase protein 3 [Caenorhabditis elegans] sp|P34546|VATL2_CAEEL Vacuolar ATP synthase 16 kDa proteolipid subunit 2/3 dbj|BAA22596.1| VHA-2 [Caenorhabditis elegans] dbj|BAA75066.1| Vha3 protein [Caenorhabditis elegans] 3.0E-70
blast CAE70304 Hypothetical protein CBG16825 [Caenorhabditis briggsae] emb|CAE65134.1| Hypothetical protein CBG10000 [Caenorhabditis briggsae] 1.0E-68
blast Q17046 Vacuolar ATP synthase 16 kDa proteolipid subunit gb|AAA29372.1| gene-12 encoded protein 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR002379 EMBL-EBI H+-transporting two-sector ATPase, C subunit 2.7E-16
Pfam IPR002379 EMBL-EBI H+-transporting two-sector ATPase, C subunit 1.4E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
R10E11.2.2 4 15-37,58-78,98-120,133-155
R10E11.2.1 4 15-37,58-78,98-120,133-155
Sequence
Target Sequence 
>C. ELEGANS:175633_AT
gaacatcctaaatcgtcttcttatcttccaatcgctcacataattcattcccaaattttc
caagtcttaacggatttccgtgtatcaatgaatgtattttcccaccttttctatcaaatt
agcccttccagtcaattcccccgccacctccctttccaatcatcagcacttgaccgatac
agtcaacgcatctcagttgactccaatatttttccccgtctgatgttcttcttgtgttag
tgaccttctcaatcatttctccccaaaattttttctctatcaatgtgtactaacattgcc
aattctacgacgacttgtctccgttttagtgtatgaattatatacatatatataattttt
aatttaaaattgcatcctattcggctaatagagctcacttatatcaacaaaaaaaggttg
tgtgctatcttgtgctttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACATCCTAAATCGTCTTCTTATC 61 351 496 Antisense
CAATCGCTCACATAATTCATTCCCA 82 183 524 Antisense
CTATCAAATTAGCCCTTCCAGTCAA 502 223 606 Antisense
CCCTTTCCAATCATCAGCACTTGAC 109 259 645 Antisense
GACCGATACAGTCAACGCATCTCAG 591 381 667 Antisense
CAGTTGACTCCAATATTTTTCCCCG 216 207 689 Antisense
TCTTCTTGTGTTAGTGACCTTCTCA 362 617 722 Antisense
AACATTGCCAATTCTACGACGACTT 563 137 787 Antisense
TACGACGACTTGTCTCCGTTTTAGT 234 111 801 Antisense
AAAATTGCATCCTATTCGGCTAATA 176 135 861 Antisense
GTTGTGTGCTATCTTGTGCTTTCCA 214 437 912 Antisense
Sequence Source GenBank