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[175622_at] protein kinase

Description g6730933 /REP_DB=GenBank Identifier /GB=AB012700.1 /PROTEIN_GB=BAA89795.1 /CHR=X /FEA=Mapped Transcript /DEF=FLR-4
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 41      4grp_high_8_8 > 32      6grp_highest_nonorm > 80      6grp_total > 30      4grp_total > 55      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 300.5
(3)
P 		92.9
(-2.7)
P 		136.3
(-1.5)
P 		81.7
(-3)
A 		219
B 321.7
(3)
P 		133.4
(-1.5)
P 		134.3
(-1.4)
P 		68.1
(-3)
A 		254
C 360
(3)
P 		128
(-2.7)
P 		148
(-2.2)
P 		117.4
(-3)
P 		243
avr 327.4
(3) 		118.1
(-2.3) 		139.5
(-1.7) 		89.1
(-3) 		238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 352
1 2 3 4 5 6 range
A 194
(-0.5)
P 		238.7
(0.8)
P 		313.9
(3)
P 		262.8
(1.5)
P 		170.8
(-1.2)
P 		110.8
(-3)
P 		203
B 192.6
(-0.7)
P 		325.2
(1.5)
P 		412.3
(3)
P 		307.6
(1.2)
P 		126
(-1.9)
P 		60.6
(-3)
P 		352
C 174.6
(-0.5)
P 		322
(3)
P 		318.6
(2.9)
P 		297.1
(2.4)
P 		92.1
(-2.4)
P 		67.9
(-3)
P 		254
avr 187.1
(-0.6) 		295.3
(1.8) 		348.3
(3) 		289.2
(1.7) 		129.6
(-1.9) 		79.8
(-3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 109.4
(3)
P 		20.2
(-2.5)
P 		35.6
(-1.5)
P 		12
(-3)
A 		97
B 102.6
(3)
P 		32.9
(-1.7)
P 		31.1
(-1.9)
P 		14.5
(-3)
A 		88
C 119.2
(3)
P 		36.8
(-2)
P 		51.6
(-1.1)
P 		19.8
(-3)
P 		99
avr 110.4
(3) 		30
(-2.1) 		39.4
(-1.5) 		15.4
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 35.8
(-2.1)
P 		84.9
(3)
P 		68.4
(1.3)
P 		66.6
(1.1)
P 		51.5
(-0.5)
P 		27
(-3)
P 		58
B 67
(0.1)
P 		108.3
(3)
P 		100.1
(2.4)
P 		81.5
(1.1)
P 		51.2
(-1)
P 		22.6
(-3)
P 		86
C 64.7
(0.3)
P 		98.3
(3)
P 		81.1
(1.6)
P 		59.8
(-0.1)
P 		29.8
(-2.4)
P 		22.7
(-3)
P 		76
avr 55.8
(-0.4) 		97.2
(3) 		83.2
(1.9) 		69.3
(0.7) 		44.2
(-1.4) 		24.1
(-3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175622_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20015
Sequence Type Exemplar sequence
Representative Public ID 6730933 NCBI
Target Description g6730933 /REP_DB=GenBank Identifier /GB=AB012700.1 /PROTEIN_GB=BAA89795.1 /CHR=X /FEA=Mapped Transcript /DEF=FLR-4
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078107(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078107 NCBI Caenorhabditis elegans FLuoRide resistant family member (flr-4) (flr-4) mRNA, complete cds. 11/11 None
GENEFINDER00000031089 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:15084812:15093291:1 9/11 None
SNAP00000031082 ENSEMBL cdna:SNAP chromosome:CEL140:X:15089428:15093291:1 9/11 None
F09B12.6 ENSEMBL cdna:known chromosome:CEL140:X:15089313:15093501:1 gene:F09B12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15089311-15093501(+) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Gene Symbol flr-4
Ensembl F09B12.6
Entrez Gene 181604 Entrez gene
SwissProt Q9NLA1 EMBL-EBI
WormBase CE26710 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5623 cell inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC35810 Hypothetical protein F09B12.6 [Caenorhabditis elegans] ref|NP_510508.1| FLuoRide resistant family member (flr-4) [Caenorhabditis elegans] sp|Q9NLA1|FLR4_CAEEL Serine/threonine-protein kinase flr-4 (Fluoride resistant protein 4) dbj|BAA89795.1| FLR-4 [Caenorhabditis elegans] 0.0
blast CAE63261 Hypothetical protein CBG07634 [Caenorhabditis briggsae] 0.0
blast CAE63261 Hypothetical protein CBG07634 [Caenorhabditis briggsae] 1.0E-172
blast CAB05479 Hypothetical protein F09B12.5 [Caenorhabditis elegans] emb|CAB08563.1| Hypothetical protein F09B12.5 [Caenorhabditis elegans] ref|NP_510507.1| F09B12.5 [Caenorhabditis elegans] 1.0E-110
blast CAE65577 Hypothetical protein CBG10576 [Caenorhabditis briggsae] 4.0E-26
Protein Families
Method ID Description E-Value
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 3.0E-17
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 0.012
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-37
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 4.3E-5
Pfam IPR000719 EMBL-EBI Protein kinase 0.012
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-37
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510508.1 3 399-421,428-450,470-492
F09B12.6 3 399-421,428-450,470-492
GENEFINDER00000031089 3 731-753,760-782,802-824
SNAP00000031082 3 299-321,328-350,370-392
Sequence
Target Sequence 
>C. ELEGANS:175622_AT
ttatcattgggcatcacggcacttggctctttcttgctaatttcttactttgttgtccga
tttgtacgctatctgattgcaatcaactgcaactgtgatcttatgcagcctcagtatttg
atcatttcggggattttgatagtgctgatgtttgcgctcttgttcagttgctgtatggtt
gctcttggagaatacaaatttcggatggcaaaccaaactttggatggttctaaattctac
ttaccccgcccccaaaagtcagcagtcctatgcggcataactgtgataactggtaaagaa
gatgccaaagacactgctcagaacatggaggaagaaatacatctaacaccctctgtcaga
aggaaccatgatgattattactacgacgagtcgtctggaccagcaaatgaagaaaactag
gaaaatattaaactaatttaatgatgcaaaacattgctagatcacgattctacataattt
cacttcaggaacttccatgtttttcacaaaaatcatcattttttccatgtcaccttattc
caatatatttatgtatcgatatgaattcactcaaactctgattctcgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATCATTGGGCATCACGGCACTTG 674 677 1422 Antisense
CACTTGGCTCTTTCTTGCTAATTTC 30 197 1441 Antisense
GTTGTCCGATTTGTACGCTATCTGA 624 437 1473 Antisense
GCAACTGTGATCTTATGCAGCCTCA 71 321 1510 Antisense
GATAGTGCTGATGTTTGCGCTCTTG 615 425 1559 Antisense
TTGCGCTCTTGTTCAGTTGCTGTAT 708 701 1573 Antisense
GTTGCTGTATGGTTGCTCTTGGAGA 400 439 1588 Antisense
AGTCAGCAGTCCTATGCGGCATAAC 247 61 1678 Antisense
ACATCTAACACCCTCTGTCAGAAGG 164 105 1760 Antisense
TTTTTTCCATGTCACCTTATTCCAA 622 675 1940 Antisense
ATTCACTCAAACTCTGATTCTCGTT 424 11 1986 Antisense
Sequence Source GenBank