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[175619_s_at]

Description g533166 /REP_DB=GenBank Identifier /GB=L36035.1 /PROTEIN_GB=AAA28077.1 /CHR=3 /FEA=Mapped Transcript
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 11      6grp_total > 06      4grp_total > 58      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3378
1 2 3 4 range
A 2414.5
(0.3)
P 		3506.4
(2.3)
P 		3864.1
(3)
P 		676.6
(-3)
P 		3188
B 2077.1
(-0.5)
P 		3114.3
(1.5)
P 		3917.8
(3)
P 		763.8
(-3)
P 		3154
C 2172.7
(-0.1)
P 		3634.6
(2.5)
P 		3932.4
(3)
P 		554.9
(-3)
P 		3378
avr 2221.4
(-0.1) 		3418.4
(2.1) 		3904.8
(3) 		665.1
(-3) 		3240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2854
1 2 3 4 5 6 range
A 1211.4
(-1.5)
P 		2350.8
(3)
P 		1057
(-2.2)
P 		1266.8
(-1.3)
P 		844.4
(-3)
P 		1511.6
(-0.3)
P 		1506
B 1641.4
(3)
P 		1435.9
(1.4)
P 		1330.5
(0.6)
P 		977.3
(-2.2)
P 		877.5
(-3)
P 		873.8
(-3)
P 		768
C 3684
(3)
P 		1349.7
(-1.9)
P 		1979.5
(-0.6)
P 		830.1
(-3)
P 		1162.5
(-2.3)
P 		869.6
(-2.9)
P 		2854
avr 2178.9
(3) 		1712.1
(0.7) 		1455.7
(-0.6) 		1024.7
(-2.7) 		961.5
(-3) 		1085
(-2.4) 		1217
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1277
1 2 3 4 range
A 879.1
(2.1)
P 		761.1
(1.4)
P 		1010.1
(3)
P 		99
(-3)
P 		911
B 662.4
(1)
P 		769.2
(1.9)
P 		907.8
(3)
P 		162.8
(-3)
P 		745
C 719.2
(-0.1)
P 		1045
(1.5)
P 		1370.6
(3)
P 		93.6
(-3)
P 		1277
avr 753.6
(0.9) 		858.4
(1.5) 		1096.2
(3) 		118.5
(-3) 		978

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1199
1 2 3 4 5 6 range
A 223.3
(-3)
P 		836.1
(3)
P 		230.2
(-2.9)
P 		321.3
(-2)
P 		254.8
(-2.7)
P 		368
(-1.6)
P 		613
B 571.1
(3)
P 		478
(1.2)
P 		323.1
(-1.8)
P 		258.8
(-3)
P 		356.5
(-1.1)
P 		325.8
(-1.7)
P 		312
C 1365.5
(3)
P 		411.8
(-1.8)
P 		503.8
(-1.3)
P 		166.9
(-3)
P 		376.2
(-2)
P 		291.1
(-2.4)
P 		1199
avr 720
(3) 		575.3
(1.2) 		352.4
(-1.7) 		249
(-3) 		329.2
(-2) 		328.3
(-2) 		471
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175619_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19833
Sequence Type Exemplar sequence
Representative Public ID 533166 NCBI
Target Description g533166 /REP_DB=GenBank Identifier /GB=L36035.1 /PROTEIN_GB=AAA28077.1 /CHR=3 /FEA=Mapped Transcript
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065028(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065028 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-60) (hsp-60) mRNA, complete cds. 11/11 A 
GENEFINDER00000002278 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1619748:1625395:1 11/11 None
SNAP00000002268 ENSEMBL cdna:SNAP chromosome:CEL140:III:1623117:1636353:1 11/11 None
Y22D7AL.5.3 ENSEMBL cdna:known chromosome:CEL140:III:1619731:1625774:1 gene:Y22D7AL.5 11/11 None
Y22D7AL.5.2 ENSEMBL cdna:known chromosome:CEL140:III:1619734:1625780:1 gene:Y22D7AL.5 11/11 None
Y22D7AL.5.1 ENSEMBL cdna:known chromosome:CEL140:III:1619739:1625784:1 gene:Y22D7AL.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1619736-1625448(+) 97.35 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hsp-60
Ensembl Y22D7AL.5
Entrez Gene 175316 Entrez gene
SwissProt P50140 EMBL-EBI
WormBase CE27244 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245164_AT chaperonin, putative at
ATGENOME1:19430_AT chaperonin, putative at
DROSOPHILA_2:1641286_S_AT Heat shock protein 60 dm
DROSGENOME1:141664_AT Heat shock protein 60 dm
MU11KSUBB:MSA.42608.0_F_AT chaperonin mm
MU19KSUBB:TC32589_AT PREDICTED: similar to 60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65) [Mus musculus], mRNA sequence mm
YEAST_2:1772196_AT Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; similarity to groEL Sc
Protein Similarities
Method ID Description E-Value
blast AAK84594 Heat shock protein protein 60 [Caenorhabditis elegans] ref|NP_497429.1| Heat Shock Protein family member (hsp-60) [Caenorhabditis elegans] sp|P50140|CH60_CAEEL Chaperonin homolog HSP60, mitochondrial precursor (Heat shock protein 60) (HSP-60) 0.0
blast AAA28077 homologous to chaperonin protein 0.0
blast CAE66431 Hypothetical protein CBG11701 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 2.9E-106
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 6.7E-101
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 1.7E-91
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175619_S_AT
gagcgtctcgccaagttgagcaagggagtcgctgtgctcaagattggaggaggatctgaa
gttgaagttggagagaagaaggaccgtgtcaccgatgctctgtgtgccacgcgtgccgct
gtcgaagaaggaatcgttccaggaggaggtgttgctcttctcagatctctgacagcactc
aaaaactacaaagcagccaacgaggatcaacagatcggtgtgaatatcgtcaagaaggct
ctcactcagccaatcgccacaatcgtcaaaaatgccggtcttgagccatcgtcgattatt
gatgaagtcaccggaaacagcaatacatcatatggttatgatgcgctcaacggaaagttc
gttgacatgttcgaggctggaattatcgatccaactaaggtggttcgcacagctctccaa
gatgcttcaggagtcgcctcacttctcgccacaaccgaatgcgtagtcaccgaaattcca
aaggaagaagcagttggtggaccagccggtggaatgggtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGTCTCGCCAAGTTGAGCAAGG 292 387 1170 Antisense
GCAAGGGAGTCGCTGTGCTCAAGAT 676 319 1189 Antisense
GAAGAAGGACCGTGTCACCGATGCT 330 337 1244 Antisense
CGCGTGCCGCTGTCGAAGAAGGAAT 94 257 1279 Antisense
CAGGAGGAGGTGTTGCTCTTCTCAG 301 209 1309 Antisense
GCTCTTCTCAGATCTCTGACAGCAC 126 301 1323 Antisense
ATCGTCAAGAAGGCTCTCACTCAGC 649 35 1395 Antisense
GGTCTTGAGCCATCGTCGATTATTG 506 501 1446 Antisense
CGCACAGCTCTCCAAGATGCTTCAG 246 255 1575 Antisense
GCCACAACCGAATGCGTAGTCACCG 320 277 1617 Antisense
GTGGACCAGCCGGTGGAATGGGTGG 524 489 1666 Antisense
Sequence Source GenBank