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[175614_at]

Description g973209 /REP_DB=GenBank Identifier /GB=U26735.1 /PROTEIN_GB=AAA87551.1 /CHR=X /FEA=Mapped Transcript /DEF=MEC-2
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 15      4grp_high_8_8 > 19      6grp_highest_nonorm > 90      6grp_total > 16      4grp_total > 29      6grp_highest_sub > 01_02      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 137.1
(2.7)
P 		67.7
(-2)
P 		53.7
(-3)
P 		142.1
(3)
A 		88
B 115.8
(3)
P 		105.5
(1.1)
P 		110.3
(2)
P 		83
(-3)
M 		33
C 129
(1.4)
P 		151.5
(3)
P 		66
(-3)
P 		133.9
(1.8)
P 		86
avr 127.3
(3) 		108.2
(0.7) 		76.7
(-3) 		119.7
(2.1) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 178.5
(3)
P 		176.2
(2.9)
P 		62.6
(-2.5)
P 		69
(-2.2)
P 		104.5
(-0.5)
P 		52.6
(-3)
P 		126
B 192.8
(3)
P 		173.9
(2.2)
P 		55.7
(-3)
P 		88.5
(-1.6)
P 		62.2
(-2.7)
P 		69.6
(-2.4)
P 		137
C 180.1
(3)
P 		165.6
(2.2)
P 		81.4
(-2.3)
P 		69
(-3)
P 		69.6
(-3)
P 		71.4
(-2.9)
P 		111
avr 183.8
(3) 		171.9
(2.4) 		66.6
(-2.9) 		75.5
(-2.4) 		78.8
(-2.3) 		64.5
(-3) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 49.9
(3)
P 		14.7
(-2.9)
P 		14
(-3)
P 		20.8
(-1.9)
A 		36
B 36.9
(3)
P 		26.1
(-0.4)
P 		25.6
(-0.5)
P 		17.7
(-3)
M 		19
C 42.7
(2.7)
P 		43.6
(3)
P 		23
(-2.9)
P 		22.6
(-3)
P 		21
avr 43.2
(3) 		28.1
(-1) 		20.9
(-2.9) 		20.4
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 32.9
(-0.6)
P 		62.7
(3)
P 		13.6
(-2.9)
P 		17.5
(-2.4)
P 		31.5
(-0.8)
P 		12.8
(-3)
P 		50
B 67.1
(3)
P 		57.9
(2)
P 		13.5
(-3)
P 		23.4
(-1.9)
P 		25.3
(-1.7)
P 		25.9
(-1.6)
P 		54
C 66.8
(3)
P 		50.5
(1.2)
P 		20.7
(-2.2)
P 		13.9
(-3)
P 		22.5
(-2)
P 		23.9
(-1.9)
P 		53
avr 55.6
(2.8) 		57
(3) 		15.9
(-3) 		18.3
(-2.6) 		26.4
(-1.5) 		20.9
(-2.3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175614_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19991
Sequence Type Exemplar sequence
Representative Public ID 973209 NCBI
Target Description g973209 /REP_DB=GenBank Identifier /GB=U26735.1 /PROTEIN_GB=AAA87551.1 /CHR=X /FEA=Mapped Transcript /DEF=MEC-2
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171691(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171691 NCBI Caenorhabditis elegans MEChanosensory abnormality family member (mec-2) (mec-2) mRNA, complete cds. 11/11 A 
F14D12.4a ENSEMBL cdna:known chromosome:CEL140:X:5575892:5590774:1 gene:F14D12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180826 3 1 NM_171691 175614_at (11), 188555_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5575891-5590774(+) 100.0 100.0
Public Domain and Genome References
Ensembl F14D12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82333 Mechanosensory abnormality protein 2, isoform a [Caenorhabditis elegans] ref|NP_741797.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] sp|Q27433|MEC2_CAEEL Mechanosensory protein 2 gb|AAA87552.1| MEC-2 gb|AAA87551.1| MEC-2 prf||2201490A stomatin-like protein 0.0
blast AAM54192 Mechanosensory abnormality protein 2, isoform b [Caenorhabditis elegans] ref|NP_001024566.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] 1.0E-167
blast AAV28352 Mechanosensory abnormality protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024567.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR001107 EMBL-EBI Band 7 protein 4.1E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741797.1 1 114-136
F14D12.4a 1 114-136
Sequence
Target Sequence 
>C. ELEGANS:175614_AT
cttctgatccgatagcaagccgatcaaggaacggtataactttaacgaaatggaccgtaa
tctttccgcactggtcaaaccactttgggcaggaaatgaaaaagaagatcgtgctgaaaa
agacgatagtacatcatcttgatctgctccggctcccctccctgtctgtgtgattctttt
tgtttctttcaattttatcctattattccaattcaacgctgatgcattactattgttgtc
atatttcatttctgtcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTGATCCGATAGCAAGCCGATC 195 217 1481 Antisense
AAATGGACCGTAATCTTTCCGCACT 120 121 1528 Antisense
TTTCCGCACTGGTCAAACCACTTTG 120 667 1543 Antisense
ACTGGTCAAACCACTTTGGGCAGGA 501 1 1550 Antisense
TAGTACATCATCTTGATCTGCTCCG 395 653 1607 Antisense
TCCCTGTCTGTGTGATTCTTTTTGT 33 599 1639 Antisense
GTGTGATTCTTTTTGTTTCTTTCAA 125 489 1648 Antisense
GTTTCTTTCAATTTTATCCTATTAT 136 451 1662 Antisense
ATCCTATTATTCCAATTCAACGCTG 139 39 1677 Antisense
ACGCTGATGCATTACTATTGTTGTC 536 91 1696 Antisense
GTTGTCATATTTCATTTCTGTCATT 492 437 1715 Antisense
Sequence Source GenBank