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[175608_at]

Description g1123031 /REP_DB=GenBank Identifier /GB=U38820.1 /PROTEIN_GB=AAA92436.1 /CHR=X /FEA=Mapped Transcript /DEF=KSR-1
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 69      4grp_highest_8_8 > 38      4grp_high_8_8 > 04      6grp_highest_nonorm > 82      6grp_total > 48      4grp_total > 05      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 849
1 2 3 4 range
A 478.2
(0.3)
P 		142.9
(-3)
P 		320.7
(-1.2)
P 		745.5
(3)
P 		603
B 409.3
(-1.2)
P 		225
(-2.5)
P 		158.7
(-3)
P 		992.2
(3)
P 		834
C 434.5
(-0.3)
P 		253.9
(-3)
P 		299.5
(-2.3)
P 		653.5
(3)
P 		400
avr 440.7
(-0.6) 		207.3
(-3) 		259.6
(-2.5) 		797.1
(3) 		590

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 354
1 2 3 4 5 6 range
A 323.1
(-1.6)
P 		438.2
(2.5)
P 		285.4
(-2.9)
P 		282.5
(-3)
P 		340
(-1)
P 		452.9
(3)
P 		170
B 481.8
(1.2)
P 		427
(0.3)
P 		234.8
(-3)
P 		481.2
(1.2)
P 		433.9
(0.4)
P 		588.3
(3)
P 		354
C 416
(1.3)
P 		414.4
(1.2)
P 		344.8
(-2)
P 		324.4
(-3)
P 		334.7
(-2.5)
P 		453
(3)
P 		129
avr 407
(0.4) 		426.5
(1) 		288.3
(-3) 		362.7
(-0.9) 		369.5
(-0.7) 		498.1
(3) 		210
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 174.1
(3)
P 		31
(-3)
P 		83.8
(-0.8)
P 		109.1
(0.3)
P 		143
B 130.5
(0.2)
P 		55.6
(-2.4)
P 		36.8
(-3)
P 		211.4
(3)
P 		175
C 143.8
(3)
P 		73
(-3)
P 		104.4
(-0.3)
P 		110.2
(0.2)
P 		71
avr 149.5
(3) 		53.2
(-3) 		75
(-1.6) 		143.6
(2.6) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 59.6
(-3)
P 		155.8
(3)
P 		62.2
(-2.8)
P 		71.7
(-2.2)
P 		102.6
(-0.3)
P 		110.3
(0.2)
P 		96
B 167.6
(1.1)
P 		142.1
(0.1)
P 		57
(-3)
P 		127.4
(-0.4)
P 		176.3
(1.4)
P 		219.4
(3)
P 		162
C 154.2
(3)
P 		126.4
(1.1)
P 		87.7
(-1.5)
P 		65.2
(-3)
P 		108.3
(-0.1)
P 		151.6
(2.8)
P 		89
avr 127.1
(0.8) 		141.4
(1.8) 		69
(-3) 		88.1
(-1.7) 		129.1
(0.9) 		160.4
(3) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175608_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19998
Sequence Type Exemplar sequence
Representative Public ID 1123031 NCBI
Target Description g1123031 /REP_DB=GenBank Identifier /GB=U38820.1 /PROTEIN_GB=AAA92436.1 /CHR=X /FEA=Mapped Transcript /DEF=KSR-1
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076995(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076995 NCBI Caenorhabditis elegans Kinase Suppressor of activated Ras family member (ksr-1) (ksr-1) mRNA, complete cds. 11/11 A 
F13B9.5.2 ENSEMBL cdna:known chromosome:CEL140:X:8275095:8283235:1 gene:F13B9.5 11/11 None
F13B9.5.1 ENSEMBL cdna:known chromosome:CEL140:X:8275106:8283235:1 gene:F13B9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8275104-8283234(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ksr-1
Ensembl F13B9.5
Entrez Gene 181082 Entrez gene
SwissProt Q19380 EMBL-EBI
WormBase CE25854 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.28475.1.S1_AT similar to Kinase suppressor of ras-1 (Kinase suppressor of ras) cfa
CANINE_2:CFAAFFX.28475.1.S1_S_AT similar to Kinase suppressor of ras-1 (Kinase suppressor of ras) cfa
CANINE_2:CFAAFFX.28478.1.S1_AT similar to Kinase suppressor of ras-1 (Kinase suppressor of ras) cfa
DROSOPHILA_2:1625295_AT kinase suppressor of ras dm
DROSGENOME1:143807_AT kinase suppressor of ras dm
CHICKEN:GGA.11110.1.A1_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
CHICKEN:GGA.5633.1.S1_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
CHICKEN:GGAAFFX.26564.1.S1_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
CHICKEN:GGAAFFX.26564.1.S1_S_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
CHICKEN:GGAAFFX.26564.2.S1_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
CHICKEN:GGAAFFX.26564.2.S1_S_AT similar to protein kinase related to Raf protein kinases; Method: conceptual translation supplied by author gga
HG-U95AV2:1716_AT kinase suppressor of ras hs
HC-G110:1716_AT kinase suppressor of ras hs
HU35KSUBA:U43586_AT kinase suppressor of ras hs
HUGENEFL:U43586_AT kinase suppressor of ras hs
HG-U133_PLUS_2:213769_AT kinase suppressor of ras hs
HG-U133A_2:213769_AT kinase suppressor of ras hs
HG-U133A:213769_AT kinase suppressor of ras hs
U133_X3P:HS.152094.0.A2_3P_AT kinase suppressor of ras hs
U133_X3P:HS.152094.0.A1_3P_AT kinase suppressor of ras hs
HG-U133A_2:213767_AT kinase suppressor of ras hs
HG-U133_PLUS_2:213767_AT kinase suppressor of ras hs
HG-U133A:213767_AT kinase suppressor of ras hs
HG-U133A:213770_AT kinase suppressor of ras hs
HG-U133A_2:213770_AT kinase suppressor of ras hs
HG-U133_PLUS_2:213770_AT kinase suppressor of ras hs
U133_X3P:HS.169061.0.A1_3P_AT Kinase suppressor of ras hs
HG-U133_PLUS_2:235252_AT Kinase suppressor of ras hs
HG-U133B:235252_AT Kinase suppressor of ras hs
HG-U95E:80554_S_AT Kinase suppressor of ras hs
HG-U95E:84487_AT Kinase suppressor of ras hs
HG-U95D:92089_AT Kinase suppressor of ras hs
HG-U95AV2:40212_AT Kinase suppressor of ras hs
HG-U95C:60382_AT Kinase suppressor of ras hs
HG-U95D:83092_AT Kinase suppressor of ras hs
HG-U95D:83094_G_AT Kinase suppressor of ras hs
MU11KSUBA:U43585_S_AT kinase suppressor of ras mm
MG-U74AV2:92765_S_AT kinase suppressor of ras mm
MG-U74AV2:92764_AT kinase suppressor of ras mm
MOE430A:1450114_AT kinase suppressor of ras mm
MOUSE430A_2:1450114_AT kinase suppressor of ras mm
MOUSE430_2:1450114_AT kinase suppressor of ras mm
MOE430A:1419823_S_AT kinase suppressor of ras mm
MOUSE430A_2:1419823_S_AT kinase suppressor of ras mm
MOUSE430_2:1419823_S_AT kinase suppressor of ras mm
MG-U74BV2:117128_AT Kinase suppressor of ras (Ksr), mRNA mm
MU19KSUBC:TC37659_AT Kinase suppressor of ras (Ksr), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39225 Kinase suppressor of activated ras protein 1 [Caenorhabditis elegans] ref|NP_509396.1| Kinase Suppressor of activated Ras family member (ksr-1) [Caenorhabditis elegans] gb|AAB35769.1| KSR-1 [Caenorhabditis elegans] gb|AAA92436.1| KSR-1 0.0
blast CAE74868 Hypothetical protein CBG22727 [Caenorhabditis briggsae] 0.0
blast T16050 hypothetical protein F13B9.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.3E-46
Sequence
Target Sequence 
>C. ELEGANS:175608_AT
aaccaaccctcaaagaccaatcgattcaatgtttttttccatgacaaacatgttccattt
tattgtgatattttcctccagtaatctcactacagcatcgttttctgttcaagcttctgt
attgtaacagttttgattcgacaccaaatctgtaatttcaaccaaaaaaatatattttgt
atattctggtcgtcaatgttggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCAACCCTCAAAGACCAATCGAT 633 145 2386 Antisense
AGACCAATCGATTCAATGTTTTTTT 381 69 2399 Antisense
ATGACAAACATGTTCCATTTTATTG 227 45 2426 Antisense
GTGATATTTTCCTCCAGTAATCTCA 80 483 2450 Antisense
TTCCTCCAGTAATCTCACTACAGCA 644 697 2458 Antisense
TAATCTCACTACAGCATCGTTTTCT 378 635 2467 Antisense
CATCGTTTTCTGTTCAAGCTTCTGT 691 211 2481 Antisense
TCTGTTCAAGCTTCTGTATTGTAAC 245 607 2489 Antisense
TGTAACAGTTTTGATTCGACACCAA 576 559 2508 Antisense
GATTCGACACCAAATCTGTAATTTC 480 431 2520 Antisense
GTATATTCTGGTCGTCAATGTTGGC 595 453 2564 Antisense
Sequence Source GenBank