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[175606_at]

Description g1381135 /REP_DB=GenBank Identifier /GB=U58084.1 /PROTEIN_GB=AAC47121.1 /CHR=3 /FEA=Mapped Transcript /DEF=CUL-2
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 84      6grp_total > 38      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 676.2
(3)
P 		492.4
(-3)
P 		616.7
(1.1)
P 		555.3
(-0.9)
P 		184
B 701.8
(3)
P 		590.7
(-0.3)
P 		500.9
(-3)
P 		594.2
(-0.2)
P 		201
C 651.5
(3)
P 		640.8
(2.6)
P 		483.1
(-3)
P 		484.4
(-3)
P 		168
avr 676.5
(3) 		574.6
(-1.3) 		533.6
(-3) 		544.6
(-2.5) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 555
1 2 3 4 5 6 range
A 917.2
(3)
P 		571.4
(-0.7)
P 		362.5
(-3)
P 		393.1
(-2.7)
P 		579.9
(-0.6)
P 		679.7
(0.4)
P 		555
B 692.6
(3)
P 		553.9
(-0.4)
P 		445.2
(-3)
P 		651.1
(2)
P 		505.9
(-1.5)
P 		624
(1.3)
P 		247
C 611.5
(2.7)
P 		617.6
(3)
P 		487.7
(-3)
P 		547.2
(-0.3)
P 		494.6
(-2.7)
P 		611.8
(2.7)
P 		130
avr 740.4
(3) 		581
(-0.1) 		431.8
(-3) 		530.5
(-1.1) 		526.8
(-1.2) 		638.5
(1) 		309
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 246.2
(3)
P 		106.9
(-2.1)
P 		161.2
(-0.1)
P 		81.3
(-3)
P 		165
B 223.8
(3)
P 		145.9
(-1.3)
P 		116.1
(-3)
P 		126.6
(-2.4)
P 		108
C 215.6
(3)
P 		184.2
(1.6)
P 		168.4
(0.9)
P 		81.7
(-3)
P 		134
avr 228.5
(3) 		145.7
(-0.8) 		148.6
(-0.6) 		96.5
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 169.1
(1.4)
P 		203.2
(3)
P 		78.9
(-3)
P 		99.7
(-2)
P 		175
(1.6)
P 		165.5
(1.2)
P 		124
B 241
(3)
P 		184.4
(0.4)
P 		108.1
(-3)
P 		172.4
(-0.1)
P 		205.5
(1.4)
P 		232.7
(2.6)
P 		133
C 226.6
(3)
P 		188.4
(1)
P 		124.1
(-2.3)
P 		110
(-3)
P 		160
(-0.4)
P 		204.8
(1.9)
P 		117
avr 212.2
(3) 		192
(1.9) 		103.7
(-3) 		127.4
(-1.7) 		180.2
(1.2) 		201
(2.4) 		109
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175606_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19786
Sequence Type Exemplar sequence
Representative Public ID 1381135 NCBI
Target Description g1381135 /REP_DB=GenBank Identifier /GB=U58084.1 /PROTEIN_GB=AAC47121.1 /CHR=3 /FEA=Mapped Transcript /DEF=CUL-2
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067424(11), NM_001027838(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067424 NCBI Caenorhabditis elegans CULlin family member (cul-2) (cul-2) mRNA, complete cds. 11/11 A 
NM_001027838 NCBI Caenorhabditis elegans CULlin family member (cul-2) (cul-2) mRNA, complete cds. 11/11 None
ZK520.4a ENSEMBL cdna:known chromosome:CEL140:III:13686715:13692104:1 gene:ZK520.4 11/11 A 
ZK520.4d ENSEMBL cdna:known chromosome:CEL140:III:13687247:13692104:1 gene:ZK520.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176806 4 2 NM_001027838 175606_at (11)
NM_067424 175606_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13687240-13692096(+) 99.85 99.85
Public Domain and Genome References
Ensembl ZK520.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70188 Hypothetical protein ZK520.4a [Caenorhabditis elegans] ref|NP_499825.3| CULlin family member (cul-2) [Caenorhabditis elegans] 0.0
blast CAD18893 Hypothetical protein ZK520.4c [Caenorhabditis elegans] ref|NP_001023008.1| CULlin family member (cul-2) [Caenorhabditis elegans] 0.0
blast CAB07302 Hypothetical protein ZK520.4b [Caenorhabditis elegans] ref|NP_001023007.1| CULlin family member (cul-2) [Caenorhabditis elegans] 0.0
blast CAD45612 Hypothetical protein ZK520.4d [Caenorhabditis elegans] ref|NP_001023009.1| CULlin family member (cul-2) [Caenorhabditis elegans] 0.0
blast AAC47121 CUL-2 0.0
blast Q17390 Cullin-2 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 1.00000002374223E-32
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 4.00000009496891E-32
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Pfam IPR001373 EMBL-EBI Cullin 1.4E-110
Sequence
Target Sequence 
>C. ELEGANS:175606_AT
ttgcgtgttttttccctgagaggcaaaaaactaaaatttaaaatttacactgtgtaaatg
actctgaaacatgccattcaaaatccccactataattttatttttcaagattttctaaca
atgattgctttttttccaaattgaactgtactgaaagtaaatcccctcgcccctcatctt
acatattttatatcattttttgctggagctgcgcaacggggaatcatcaaactgtttatt
ggaatacctatttttatacttttggagccctcaaaaactgaaaatcgcgggaaattctct
gttactcccgacaaacggctatttgcctataaaaccaaccagtccgtagcctctccttga
aaatgtatatgtactatatttgtcttttttttgtgttctatcctccctcctcaagcaact
attttttctccggaatctctatgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCGTGTTTTTTCCCTGAGAGGCA 477 689 2282 Antisense
GAAACATGCCATTCAAAATCCCCAC 198 357 2347 Antisense
GCCCCTCATCTTACATATTTTATAT 118 287 2450 Antisense
GGAGCTGCGCAACGGGGAATCATCA 264 521 2486 Antisense
TATTTTTATACTTTTGGAGCCCTCA 14 663 2530 Antisense
GGGAAATTCTCTGTTACTCCCGACA 683 489 2570 Antisense
ACTCCCGACAAACGGCTATTTGCCT 179 95 2585 Antisense
TATAAAACCAACCAGTCCGTAGCCT 40 653 2609 Antisense
CCGTAGCCTCTCCTTGAAAATGTAT 274 265 2625 Antisense
TCCCTCCTCAAGCAACTATTTTTTC 644 597 2685 Antisense
TTTTTTCTCCGGAATCTCTATGATT 380 675 2703 Antisense
Sequence Source GenBank