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[175600_s_at]

Description g561675 /REP_DB=GenBank Identifier /GB=U15667.1 /PROTEIN_GB=AAA51021.1 /CHR=1 /FEA=Mapped Transcript /DEF=CeSema
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 59      4grp_highest_8_8 > 07      4grp_high_8_8 > 28      6grp_highest_nonorm > 72      6grp_total > 01      4grp_total > 27      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 386.3
(-0.4)
P 		454.8
(3)
P 		335.5
(-3)
P 		430.3
(1.8)
P 		119
B 365.2
(-2.6)
P 		452.4
(1.8)
P 		358.3
(-3)
P 		474.9
(3)
P 		117
C 426.1
(-0.9)
P 		463.5
(0.5)
P 		367.9
(-3)
P 		532.8
(3)
P 		165
avr 392.5
(-1.2) 		456.9
(1.9) 		353.9
(-3) 		479.3
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 342.5
(-1.2)
P 		500.5
(3)
P 		274.5
(-3)
P 		385.8
(-0)
P 		440.5
(1.4)
P 		462.7
(2)
P 		226
B 414.5
(-0.2)
P 		469.2
(3)
P 		366.7
(-3)
P 		407.5
(-0.6)
P 		447.6
(1.7)
P 		383.4
(-2)
P 		103
C 437.2
(0.8)
P 		473.9
(3)
P 		427.6
(0.2)
P 		375
(-3)
P 		468
(2.6)
P 		465.2
(2.5)
P 		99
avr 398.1
(-1) 		481.2
(3) 		356.3
(-3) 		389.4
(-1.4) 		452
(1.6) 		437.1
(0.9) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 140.7
(3)
P 		98.7
(-0.2)
P 		87.7
(-1.1)
P 		63
(-3)
P 		78
B 116.5
(3)
P 		111.7
(2.1)
P 		83
(-3)
P 		101.2
(0.3)
P 		34
C 141
(3)
P 		133.3
(2.1)
P 		128.2
(1.5)
P 		89.8
(-3)
P 		51
avr 132.7
(3) 		114.6
(0.7) 		99.6
(-1.1) 		84.7
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 63.1
(-2.8)
P 		178
(3)
P 		59.8
(-3)
P 		97.8
(-1.1)
P 		132.9
(0.7)
P 		112.6
(-0.3)
P 		118
B 144.2
(0.6)
P 		156.2
(1.3)
P 		89.1
(-3)
P 		107.9
(-1.8)
P 		181.9
(3)
P 		143
(0.5)
P 		93
C 162
(3)
P 		144.6
(1.8)
P 		108.8
(-0.7)
P 		75.4
(-3)
P 		151.4
(2.3)
P 		155.7
(2.6)
P 		87
avr 123.1
(0) 		159.6
(3) 		85.9
(-3) 		93.7
(-2.4) 		155.4
(2.7) 		137.1
(1.2) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175600_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19696
Sequence Type Exemplar sequence
Representative Public ID 561675 NCBI
Target Description g561675 /REP_DB=GenBank Identifier /GB=U15667.1 /PROTEIN_GB=AAA51021.1 /CHR=1 /FEA=Mapped Transcript /DEF=CeSema
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061182(11), NM_061181(11), U15667(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061182 NCBI Caenorhabditis elegans SeMaPhorin related family member (smp-1) (smp-1) mRNA, complete cds. 11/11 None
NM_061181 NCBI Caenorhabditis elegans SeMaPhorin related family member (smp-1) (smp-1) mRNA, complete cds. 11/11 A 
U15667 NCBI Caenorhabditis elegans semaphorin CeSema (cesema) mRNA, complete cds. 11/11 A 
GENEFINDER00000016375 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14817052:14823448:1 11/11 None
SNAP00000016370 ENSEMBL cdna:SNAP chromosome:CEL140:I:14818503:14823448:1 11/11 None
Y54E5B.1b ENSEMBL cdna:known chromosome:CEL140:I:14815585:14821947:1 gene:Y54E5B.1 11/11 None
Y54E5B.1a ENSEMBL cdna:known chromosome:CEL140:I:14815586:14823832:1 gene:Y54E5B.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14815515-14821878(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54E5B.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21714 Hypothetical protein Y54E5B.1a [Caenorhabditis elegans] ref|NP_493582.3| SeMaPhorin related family member (smp-1) [Caenorhabditis elegans] sp|Q17330|SEM1A_CAEEL Semaphorin 1A precursor (CeSema) 0.0
blast CAE72526 Hypothetical protein CBG19706 [Caenorhabditis briggsae] 0.0
blast AAA51021 CeSema 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 5.8E-85
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.0E-126
Pfam IPR002165 EMBL-EBI Plexin 1.1E-10
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.0E-126
Pfam IPR002165 EMBL-EBI Plexin 1.3E-5
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.8E-67
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 5.6E-65
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 9.1E-45
Pfam IPR002165 EMBL-EBI Plexin 1.1E-10
Pfam IPR001627 EMBL-EBI Semaphorin/CD100 antigen 1.0E-126
Pfam IPR002165 EMBL-EBI Plexin 1.1E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493582.3 1 580-602
Y54E5B.1a 1 580-602
GENEFINDER00000016375 1 637-659
SNAP00000016370 1 564-586
Sequence
Target Sequence 
>C. ELEGANS:175600_S_AT
ataaccacgtcgtctacactgtattctcaactccggactctgatgttcgaatgagtgctg
tttgcaagttttcgatgaagaagattcgcgaggagtttgataatggaacttttaagcaca
gaataatgcccaatcaatgtggatggcattcaatcggaacgaagtaccaaaacctcgtcc
tggatcgtgttctccagattctacaaaacttcctgaaaatactgtatcattcattcttca
tcatccattacttcatcgtccgattccttctgtagctgcacctcttttagttgaaggtgc
agatcgtgcagatctaactcagattactgtacttccacgtgtacgtgcagttggtggaca
taactatgatattctgttcattggaacgtccgatggaaaagttctgaaagttgttgaagt
ggatggtaatgctacagtaatccaatcggctacagtattccaaagaggtgttccaattgt
caatctactcacaactaaggaaagtgttgtaattgtgtctgctgacgaaattgcatctct
accagttcataattgtgcacaacagacgagctgctccaaatgtgtacaactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAACCACGTCGTCTACACTGTATT 529 25 967 Antisense
CTCAACTCCGGACTCTGATGTTCGA 37 229 992 Antisense
CAAAACCTCGTCCTGGATCGTGTTC 325 191 1134 Antisense
TGTAGCTGCACCTCTTTTAGTTGAA 18 559 1237 Antisense
GATTACTGTACTTCCACGTGTACGT 196 431 1288 Antisense
GTAATCCAATCGGCTACAGTATTCC 293 463 1403 Antisense
GTGTTCCAATTGTCAATCTACTCAC 530 485 1434 Antisense
GTGTTGTAATTGTGTCTGCTGACGA 280 485 1470 Antisense
GCATCTCTACCAGTTCATAATTGTG 677 309 1499 Antisense
TGTGCACAACAGACGAGCTGCTCCA 307 557 1520 Antisense
GAGCTGCTCCAAATGTGTACAACTT 502 387 1534 Antisense
Sequence Source GenBank