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[175597_at]

Description g1354761 /REP_DB=GenBank Identifier /GB=U56864.1 /PROTEIN_GB=AAB04059.1 /CHR=1 /FEA=Mapped Transcript /DEF=EGL-30
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 412
1 2 3 4 range
A 605.4
(3)
P 		464.4
(0.6)
P 		474.3
(0.8)
P 		251.9
(-3)
P 		354
B 663.4
(3)
P 		501
(0.4)
P 		358.3
(-2)
P 		295.2
(-3)
P 		368
C 647.9
(3)
P 		541.5
(1.2)
P 		466.3
(-0.1)
P 		294.2
(-3)
P 		354
avr 638.9
(3) 		502.3
(0.7) 		433
(-0.4) 		280.4
(-3) 		359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 900
1 2 3 4 5 6 range
A 830
(3)
P 		501.5
(-0.2)
P 		506.1
(-0.1)
P 		327.2
(-1.8)
P 		503.4
(-0.1)
P 		207.1
(-3)
P 		623
B 1107
(3)
P 		660.8
(-0.4)
P 		433.2
(-2.1)
P 		522.8
(-1.5)
P 		391
(-2.5)
P 		321.6
(-3)
P 		785
C 783.2
(3)
P 		786.6
(3)
P 		474.2
(-0.6)
P 		329.4
(-2.3)
P 		300.4
(-2.6)
P 		270.1
(-3)
P 		517
avr 906.7
(3) 		649.6
(0.6) 		471.2
(-1.1) 		393.1
(-1.8) 		398.3
(-1.8) 		266.3
(-3) 		640
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 220.4
(3)
P 		100.8
(-0.9)
P 		124
(-0.2)
P 		36.9
(-3)
P 		184
B 211.5
(3)
P 		123.7
(-0.5)
P 		83
(-2.2)
P 		62.9
(-3)
P 		149
C 214.5
(3)
P 		155.7
(0.9)
P 		162.5
(1.1)
P 		49.6
(-3)
P 		165
avr 215.5
(3) 		126.7
(-0.2) 		123.2
(-0.3) 		49.8
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 335
1 2 3 4 5 6 range
A 153
(1.8)
P 		178.3
(3)
P 		110.2
(-0.2)
P 		83
(-1.5)
P 		151.9
(1.8)
P 		50.4
(-3)
P 		128
B 385.2
(3)
P 		220
(-0.5)
P 		105.2
(-3)
P 		138.5
(-2.3)
P 		158.9
(-1.8)
P 		119.9
(-2.7)
P 		280
C 290.3
(3)
P 		240
(1.7)
P 		120.7
(-1.5)
P 		66.2
(-3)
P 		97.2
(-2.2)
P 		90.4
(-2.4)
P 		224
avr 276.2
(3) 		212.8
(1) 		112
(-2.2) 		95.9
(-2.7) 		136
(-1.4) 		86.9
(-3) 		189
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175597_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19626
Sequence Type Exemplar sequence
Representative Public ID 1354761 NCBI
Target Description g1354761 /REP_DB=GenBank Identifier /GB=U56864.1 /PROTEIN_GB=AAB04059.1 /CHR=1 /FEA=Mapped Transcript /DEF=EGL-30
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170806(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170806 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-30) (egl-30) mRNA, complete cds. 11/11 A 
M01D7.7a ENSEMBL cdna:known chromosome:CEL140:I:1835680:1841924:1 gene:M01D7.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000009603 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171751 2 1 NM_170806 175597_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1835720-1841192(+) 94.1 99.69
Public Domain and Genome References
Ensembl M01D7.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB58071 Egg laying defective protein 30, isoform a [Caenorhabditis elegans] ref|NP_740789.1| EGg Laying defective family member (egl-30) [Caenorhabditis elegans] gb|AAG32092.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] gb|AAB04059.1| EGL-30 0.0
blast AAW02891 egl-30 [Caenorhabditis briggsae] emb|CAE74465.1| Hypothetical protein CBG22211 [Caenorhabditis briggsae] 0.0
blast AAM15593 Egg laying defective protein 30, isoform b [Caenorhabditis elegans] ref|NP_001021574.1| EGg Laying defective family member (egl-30) [Caenorhabditis elegans] 1.0E-174
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175597_AT
gaagaaagtcgcatgtcggattggatgatgatgatgatgatccatctctctctctctctc
tctctcactgggtcgagtgagacaccaccacctaaacctaggaaacattttcttgtactc
cttctaatttttgttttttttttgcaaaaaactttctctctctgtctgtctctctctcca
tctcttccttattttcttattttctcattttcctccctaaaacaaatgctcctcccgaat
attctttccatataagcacttttttcttctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAAAGTCGCATGTCGGATTGGA 38 341 1297 Antisense
GTCGCATGTCGGATTGGATGATGAT 175 475 1304 Antisense
ACTGGGTCGAGTGAGACACCACCAC 491 97 1363 Antisense
ACATTTTCTTGTACTCCTTCTAATT 595 103 1401 Antisense
TCTTGTACTCCTTCTAATTTTTGTT 71 619 1407 Antisense
GCAAAAAACTTTCTCTCTCTGTCTG 353 317 1440 Antisense
TCTCATTTTCCTCCCTAAAACAAAT 252 613 1499 Antisense
TTCCTCCCTAAAACAAATGCTCCTC 314 697 1506 Antisense
TAAAACAAATGCTCCTCCCGAATAT 207 641 1514 Antisense
TCCTCCCGAATATTCTTTCCATATA 648 593 1526 Antisense
TCCATATAAGCACTTTTTTCTTCTT 445 591 1543 Antisense
Sequence Source GenBank