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[175590_s_at]

Description CE440 /REP_DB=TREMBL Accession /GB=T00440 /CHR=2 /FEA=Genomic Cluster /DEF=wEST01161 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CEESF46, mRNA sequence.
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_high_8_8 > 51      6grp_highest_nonorm > 92      6grp_total > 78      4grp_total > 60      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 144.2
(-0.5)
P 		132.8
(-1.5)
P 		180.5
(3)
P 		117.4
(-3)
A 		63
B 174.6
(3)
P 		163.2
(2.5)
P 		113.7
(0.2)
P 		43.4
(-3)
A 		131
C 148.2
(0.2)
P 		168.5
(2.5)
P 		172.5
(3)
P 		120
(-3)
P 		53
avr 155.7
(3) 		154.8
(2.9) 		155.6
(3) 		93.6
(-3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 393
1 2 3 4 5 6 range
A 204.3
(-1.5)
P 		155.2
(-2.5)
P 		129.3
(-3)
P 		180.4
(-2)
P 		276.4
(-0)
P 		425.1
(3)
P 		296
B 235.9
(-1.4)
P 		132.4
(-3)
P 		133.2
(-3)
P 		159
(-2.6)
P 		278.1
(-0.7)
P 		512
(3)
P 		380
C 149.4
(-2.5)
P 		158.3
(-2.4)
P 		118.8
(-3)
P 		152.8
(-2.5)
P 		238.2
(-1.1)
P 		503.5
(3)
P 		385
avr 196.5
(-1.8) 		148.6
(-2.6) 		127.1
(-3) 		164.1
(-2.4) 		264.2
(-0.7) 		480.2
(3) 		353
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 52.5
(3)
P 		28.8
(-1)
P 		47.2
(2.1)
P 		17.2
(-3)
A 		35
B 55.7
(3)
P 		40.3
(1)
P 		26.3
(-0.8)
P 		9.2
(-3)
A 		47
C 49.1
(1.3)
P 		48.4
(1.2)
P 		60.1
(3)
P 		20.2
(-3)
P 		40
avr 52.4
(3) 		39.2
(0.9) 		44.5
(1.7) 		15.5
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 37.7
(-2.2)
P 		55.2
(-0.8)
P 		28.2
(-3)
P 		45.7
(-1.6)
P 		83.4
(1.4)
P 		103.5
(3)
P 		75
B 82.1
(-1.1)
P 		44.1
(-2.6)
P 		32.3
(-3)
P 		42.1
(-2.6)
P 		113
(0.1)
P 		190.9
(3)
P 		159
C 55.4
(-1.9)
P 		48.3
(-2.2)
P 		30.2
(-3)
P 		30.7
(-3)
P 		77.1
(-1)
P 		168.5
(3)
P 		138
avr 58.4
(-1.6) 		49.2
(-2.1) 		30.2
(-3) 		39.5
(-2.6) 		91.2
(-0.1) 		154.3
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175590_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20743
Sequence Type Exemplar sequence
Representative Public ID CE440 NCBI
Target Description CE440 /REP_DB=TREMBL Accession /GB=T00440 /CHR=2 /FEA=Genomic Cluster /DEF=wEST01161 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CEESF46, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062536(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062536 NCBI Caenorhabditis elegans DiSHevelled related family member (dsh-2) (dsh-2) mRNA, complete cds. 11/11 A 
C27A2.6 ENSEMBL cdna:known chromosome:CEL140:II:5078211:5081144:-1 gene:C27A2.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044054 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5078238-5078628(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dsh-2
Ensembl C27A2.6
Entrez Gene 266854 Entrez gene
SwissProt Q18239 EMBL-EBI
WormBase CE27097 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.29421.1.S1_AT similar to dishevelled 1 isoform a cfa
CHICKEN:GGA.16412.1.S1_S_AT dishevelled, dsh homolog 1 (Drosophila) gga
CHICKEN:GGAAFFX.1037.1.S1_AT dishevelled, dsh homolog 1 (Drosophila) gga
CHICKEN:GGAAFFX.20672.1.S1_AT dishevelled, dsh homolog 1 (Drosophila) gga
CHICKEN:GGAAFFX.20935.1.S1_AT dishevelled, dsh homolog 1 (Drosophila) gga
HG-U95AV2:163_AT dishevelled, dsh homolog 1 (Drosophila) hs
HC-G110:163_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U133_PLUS_2:203230_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U133A:203230_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-FOCUS:203230_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U133A_2:203230_AT dishevelled, dsh homolog 1 (Drosophila) hs
HU35KSUBD:RC_AA599296_AT dishevelled, dsh homolog 1 (Drosophila) hs
HUGENEFL:U46461_AT dishevelled, dsh homolog 1 (Drosophila) hs
U133_X3P:G2291005_3P_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U95B:43392_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U95B:45360_S_AT dishevelled, dsh homolog 1 (Drosophila) hs
HG-U95C:64927_AT dishevelled, dsh homolog 1 (Drosophila) hs
HU35KSUBA:RC_D25680_AT Dishevelled, dsh homolog 1 (Drosophila) hs
MU11KSUBA:U10115_S_AT dishevelled, dsh homolog 1 (Drosophila) mm
MG-U74AV2:98418_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOE430A:1450978_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOUSE430A_2:1450978_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOUSE430_2:1450978_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOE430A:1437301_A_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOUSE430A_2:1437301_A_AT dishevelled, dsh homolog 1 (Drosophila) mm
MOUSE430_2:1437301_A_AT dishevelled, dsh homolog 1 (Drosophila) mm
MU19KSUBC:TC36099_AT Dishevelled, dsh homolog 1 (Drosophila) (Dvl1), mRNA mm
RAE230A:1369997_AT dishevelled, dsh homolog 1 (Drosophila) rn
RAT230_2:1369997_AT dishevelled, dsh homolog 1 (Drosophila) rn
RG-U34C:RC_AI101690_AT dishevelled, dsh homolog 1 (Drosophila) rn
RAE230B:1397351_S_AT dishevelled, dsh homolog 1 (Drosophila) rn
RAT230_2:1397351_S_AT dishevelled, dsh homolog 1 (Drosophila) rn
RAE230B:1384698_AT Dishevelled, dsh homolog 1 (Drosophila) rn
RAT230_2:1384698_AT Dishevelled, dsh homolog 1 (Drosophila) rn
RG-U34B:RC_AA963810_AT Dishevelled, dsh homolog 1 (Drosophila) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7222 frizzled signaling pathway inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
7275 development inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494937 DiSHevelled related family member (dsh-2) [Caenorhabditis elegans] gb|AAK31465.1| Dishevelled related protein 2 [Caenorhabditis elegans] 0.0
blast T15652 hypothetical protein C27A2.6 - Caenorhabditis elegans 0.0
blast CAE59037 Hypothetical protein CBG02318 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-12
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 5.7E-26
Pfam IPR001158 EMBL-EBI DIX domain 5.3E-55
Sequence
Target Sequence 
>C. ELEGANS:175590_S_AT
aagcctgcatcactcagcagtgggtctggtttagctgttgatctggaccgcgtcgcttcg
agaagctcgtttcgagctgcaatgaacggaagtcatccacagtttaacattgataattga
ttagtagatgttcttgtttctctgtattttttgatatgttttaatttgtgttaactgtgt
attcctcccaacgggatatttgtcatttttaatctctcgacatccactgcctttttcatg
ttctcatctaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCCTGCATCACTCAGCAGTGGGT 614 147 59 Antisense
GTGGGTCTGGTTTAGCTGTTGATCT 402 489 78 Antisense
GTTTAGCTGTTGATCTGGACCGCGT 317 449 87 Antisense
ACCGCGTCGCTTCGAGAAGCTCGTT 514 89 105 Antisense
GCTCGTTTCGAGCTGCAATGAACGG 522 291 123 Antisense
GGAAGTCATCCACAGTTTAACATTG 137 531 146 Antisense
GTAGATGTTCTTGTTTCTCTGTATT 414 457 182 Antisense
TGTGTTAACTGTGTATTCCTCCCAA 372 555 225 Antisense
CCTCCCAACGGGATATTTGTCATTT 432 265 242 Antisense
ATTTTTAATCTCTCGACATCCACTG 119 15 263 Antisense
TGCCTTTTTCATGTTCTCATCTAAA 427 585 286 Antisense
Sequence Source Affymetrix Proprietary Database