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[175582_at]

Description CE1241 /REP_DB=TREMBL Accession /GB=T01124 /CHR=4 /FEA=Genomic Cluster /DEF=wEST01845 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CEESN08, mRNA sequence.
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 96      6grp_total > 66      4grp_total > 22      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 457
1 2 3 4 range
A 803.7
(3)
P 		501.4
(-2.9)
P 		497.9
(-3)
P 		790.7
(2.7)
P 		306
B 857.5
(2.4)
P 		553.5
(-1.6)
P 		445.7
(-3)
P 		902.6
(3)
P 		457
C 838
(3)
P 		482.5
(-3)
P 		540.7
(-2)
P 		736.8
(1.3)
P 		356
avr 833.1
(3) 		512.5
(-2.7) 		494.8
(-3) 		810
(2.6) 		338

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 814
1 2 3 4 5 6 range
A 738.6
(-1.4)
P 		754.8
(-1.3)
P 		526.5
(-3)
P 		869.8
(-0.5)
P 		741.6
(-1.4)
P 		1340.8
(3)
P 		814
B 599.8
(-3)
P 		706.7
(-1.7)
P 		657
(-2.3)
P 		735.3
(-1.3)
P 		639.8
(-2.5)
P 		1084.7
(3)
P 		485
C 695.4
(-2.1)
P 		729.5
(-1.9)
P 		755.9
(-1.6)
P 		869.7
(-0.7)
P 		592.1
(-3)
P 		1316
(3)
P 		724
avr 677.9
(-2.7) 		730.3
(-2.2) 		646.5
(-3) 		824.9
(-1.2) 		657.8
(-2.9) 		1247.2
(3) 		601
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 292.6
(3)
P 		108.8
(-3)
P 		130.2
(-2.3)
P 		115.7
(-2.8)
P 		184
B 273.5
(3)
P 		136.7
(-1.8)
P 		103.3
(-3)
P 		192.3
(0.1)
P 		170
C 277.4
(3)
P 		138.7
(-2.4)
P 		188.5
(-0.5)
P 		124.2
(-3)
P 		153
avr 281.2
(3) 		128.1
(-3) 		140.7
(-2.5) 		144.1
(-2.4) 		153

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 136.2
(-2.4)
P 		268.5
(1.4)
P 		114.7
(-3)
P 		220.6
(0)
P 		223.8
(0.1)
P 		326.4
(3)
P 		212
B 208.7
(-1.8)
P 		235.2
(-1.1)
P 		159.5
(-3)
P 		194.7
(-2.1)
P 		259.9
(-0.5)
P 		404.5
(3)
P 		245
C 257.8
(-1.1)
P 		222.6
(-1.9)
P 		192.4
(-2.6)
P 		174.9
(-3)
P 		191.6
(-2.6)
P 		440.6
(3)
P 		266
avr 200.9
(-1.8) 		242.1
(-0.8) 		155.5
(-3) 		196.7
(-1.9) 		225.1
(-1.2) 		390.5
(3) 		235
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175582_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21349
Sequence Type Exemplar sequence
Representative Public ID CE1241 NCBI
Target Description CE1241 /REP_DB=TREMBL Accession /GB=T01124 /CHR=4 /FEA=Genomic Cluster /DEF=wEST01845 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CEESN08, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027965(11), NM_001027964(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027965 NCBI Caenorhabditis elegans Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) (pme-3) mRNA, complete cds. 11/11 None
NM_001027964 NCBI Caenorhabditis elegans Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) (pme-3) mRNA, complete cds. 11/11 None
F20C5.1a.1 ENSEMBL cdna:known chromosome:CEL140:IV:8746234:8752658:1 gene:F20C5.1 11/11 A 
F20C5.1b ENSEMBL cdna:known chromosome:CEL140:IV:8746236:8752658:1 gene:F20C5.1 11/11 A 
F20C5.1a.3 ENSEMBL cdna:known chromosome:CEL140:IV:8746236:8752658:1 gene:F20C5.1 11/11 None
F20C5.1a.2 ENSEMBL cdna:known chromosome:CEL140:IV:8746274:8752807:1 gene:F20C5.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027966 5/11 Cross Hyb Matching Probes None
SNAP00000022214 5/11 Cross Hyb Matching Probes None
GENEFINDER00000022220 4/11 Cross Hyb Matching Probes None
F20C5.1c 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177683 3 2 NM_001027964 175582_at (11)
NM_001027965 175582_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8752192-8752642(+) 100.0 100.0
Public Domain and Genome References
Ensembl F20C5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92299 Hypothetical protein F20C5.1b [Caenorhabditis elegans] ref|NP_001023136.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] gb|AAO26317.1| poly ADP-ribose metabolism enzyme-3 short form [Caenorhabditis elegans] 0.0
blast CAD89735 Hypothetical protein F20C5.1a [Caenorhabditis elegans] ref|NP_001023135.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] gb|AAO26316.1| poly ADP-ribose metabolism enzyme-3 long form [Caenorhabditis elegans] sp|Q867X0|PME3_CAEEL Poly(ADP-ribose) glycohydrolase pme-3 (Poly ADP-ribose metabolism enzyme-3) 0.0
blast CAH60763 Hypothetical protein F20C5.1c [Caenorhabditis elegans] ref|NP_001023137.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175582_AT
aacacttggcatgctattcagtatgataaacaacaccggcttgccacataagcactttga
attctacgtcttcgatagaatttctacttatctcagtagttcggaagatgttgagtcttc
gaaatcatcaccttcagtatcccgagcataattcgaatcgcccacacggccataaagacc
ggttcctttcgattaaattctgttaaatatgcatgctccgtctttaaaaaatcagtcccc
gtattttaaacgttttgattttaatgttcatattattatccgaaattagtatactcgccg
tcatgaaagcccgagatatctagttcgcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACACTTGGCATGCTATTCAGTATG 681 135 13 Antisense
TGATAAACAACACCGGCTTGCCACA 246 561 36 Antisense
ACCGGCTTGCCACATAAGCACTTTG 700 87 47 Antisense
AGCACTTTGAATTCTACGTCTTCGA 692 75 63 Antisense
GAGTCTTCGAAATCATCACCTTCAG 469 399 125 Antisense
ACCTTCAGTATCCCGAGCATAATTC 143 87 142 Antisense
CGAGCATAATTCGAATCGCCCACAC 54 251 155 Antisense
CGCCCACACGGCCATAAAGACCGGT 46 257 171 Antisense
AATATGCATGCTCCGTCTTTAAAAA 28 173 218 Antisense
AGTATACTCGCCGTCATGAAAGCCC 425 61 300 Antisense
AAGCCCGAGATATCTAGTTCGCAAG 662 149 319 Antisense
Sequence Source Affymetrix Proprietary Database