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[175565_s_at] Proteasome component C5

Description CEXT00664 /REP_DB=TREMBL Accession /GB=M80127 /CHR=3 /FEA=Genomic Cluster /DEF=wEST00664 Mixed stage, Stratagene (cat. no. 937006) Caenorhabditis elegans cDNA clone CEMSH62 similar to Multicatalytic proteinase subunit C5, mRNA sequence.
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 47      6grp_total > 74      4grp_total > 32      6grp_cgc > 66      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3576
1 2 3 4 range
A 2857.2
(-3)
P 		4824.9
(1.4)
P 		5019.4
(1.8)
P 		5539.1
(3)
P 		2682
B 2771.5
(-3)
P 		4711.8
(0.3)
P 		6347.5
(3)
P 		5408.8
(1.4)
P 		3576
C 2847.5
(-3)
P 		4629.3
(2)
P 		4950.2
(2.9)
P 		4975.3
(3)
P 		2128
avr 2825.4
(-3) 		4722
(1.4) 		5439
(3) 		5307.7
(2.7) 		2614

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1922
1 2 3 4 5 6 range
A 2814.2
(-2.8)
P 		2764.8
(-3)
P 		4038
(3)
P 		3613.9
(1)
P 		3454.6
(0.3)
P 		3821.9
(2)
P 		1273
B 2755.7
(-3)
P 		3119.4
(-1.8)
P 		4649.7
(3)
P 		3899.3
(0.6)
P 		3642.9
(-0.2)
P 		3813.5
(0.4)
P 		1894
C 2727.3
(-3)
P 		3057.4
(-1.6)
P 		3840.8
(1.6)
P 		3932.1
(1.9)
P 		3446.7
(-0)
P 		4190.1
(3)
P 		1463
avr 2765.7
(-3) 		2980.5
(-2.1) 		4176.2
(3) 		3815.1
(1.5) 		3514.7
(0.2) 		3941.8
(2) 		1411
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 915
1 2 3 4 range
A 1040.3
(-0.3)
P 		1047.4
(-0.2)
P 		1312.2
(3)
P 		810.8
(-3)
P 		501
B 883.8
(-3)
P 		1163.7
(-0.1)
P 		1470.9
(3)
P 		1152.6
(-0.3)
P 		587
C 942.6
(-2.3)
P 		1331
(0.3)
P 		1725.3
(3)
P 		838.9
(-3)
P 		886
avr 955.6
(-2.8) 		1180.7
(-0.4) 		1502.8
(3) 		934.1
(-3) 		569

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 961
1 2 3 4 5 6 range
A 518.8
(-3)
P 		983.3
(2.3)
P 		879.4
(1.1)
P 		916.6
(1.6)
P 		1042.5
(3)
P 		930.5
(1.7)
P 		524
B 958.9
(-3)
P 		1038.3
(-2.1)
P 		1129.1
(-1)
P 		1032.8
(-2.1)
P 		1480.1
(3)
P 		1422
(2.3)
P 		521
C 1010.9
(-0.8)
P 		932.9
(-1.6)
P 		977.6
(-1.2)
P 		790.7
(-3)
P 		1115.3
(0.2)
P 		1402.7
(3)
P 		612
avr 829.5
(-3) 		984.8
(-0.8) 		995.4
(-0.6) 		913.4
(-1.8) 		1212.6
(2.4) 		1251.7
(3) 		422
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175565_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20997
Sequence Type Exemplar sequence
Representative Public ID CEXT00664 NCBI
Target Description CEXT00664 /REP_DB=TREMBL Accession /GB=M80127 /CHR=3 /FEA=Genomic Cluster /DEF=wEST00664 Mixed stage, Stratagene (cat. no. 937006) Caenorhabditis elegans cDNA clone CEMSH62 similar to Multicatalytic proteinase subunit C5, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066405(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066405 NCBI Caenorhabditis elegans Proteasome Beta Subunit family member (pbs-6) (pbs-6) mRNA, complete cds. 11/11 None
SNAP00000031508 ENSEMBL cdna:SNAP chromosome:CEL140:III:8243139:8244008:-1 11/11 None
GENEFINDER00000031526 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8243139:8244008:-1 11/11 None
C02F5.9.1 ENSEMBL cdna:known chromosome:CEL140:III:8243035:8244010:-1 gene:C02F5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8243122-8243449(-) 100.0 100.0
Public Domain and Genome References
Gene Title Proteasome component C5
Gene Symbol pbs-6
Ensembl C02F5.9
Entrez Gene 176161 Entrez gene
SwissProt P34286 EMBL-EBI
WormBase CE26745 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251337_AT 20S proteasome beta subunit F1 (PBF1) at
CANINE:1586828_AT proteasome (prosome, macropain) subunit, beta type, 1 cfa
CANINE_2:CFAAFFX.7079.1.S1_AT proteasome (prosome, macropain) subunit, beta type, 1 cfa
DROSOPHILA_2:1623288_AT Proteasome 26kD subunit dm
DROSGENOME1:142705_AT Proteasome 26kD subunit dm
CHICKEN:GGA.4653.2.S1_A_AT proteasome (prosome, macropain) subunit, beta type, 1 gga
HG-U133A_2:200876_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133_PLUS_2:200876_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133A:200876_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-FOCUS:200876_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U95AV2:1447_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HC-G110:1447_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HUGENEFL:D00761_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
U133_X3P:G4506192_3P_A_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133A_2:214288_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133_PLUS_2:214288_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133A:214288_S_AT proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133A_2:214289_AT Proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133_PLUS_2:214289_AT Proteasome (prosome, macropain) subunit, beta type, 1 hs
HG-U133A:214289_AT Proteasome (prosome, macropain) subunit, beta type, 1 hs
MOE430A:1420052_X_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MOUSE430A_2:1420052_X_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MOUSE430_2:1420052_X_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MOE430A:1448166_A_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MOUSE430_2:1448166_A_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MOUSE430A_2:1448166_A_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MU11KSUBB:MSA.43193.0_F_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MG-U74AV2:98113_AT proteasome (prosome, macropain) subunit, beta type 1 mm
MU11KSUBA:C81484_RC_S_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MG-U74BV2:116776_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MG-U74AV2:160875_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MOE430A:1420053_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MOUSE430A_2:1420053_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MOUSE430_2:1420053_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MG-U74AV2:161923_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MU19KSUBB:TC27792_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
MU19KSUBB:TC34588_F_AT Proteasome (prosome, macropain) subunit, beta type 1, mRNA (cDNA clone MGC:5916 IMAGE:3584538) mm
RG-U34A:RC_AA849722_AT proteasome (prosome, macropain) subunit, beta type 1 rn
RAE230A:1398812_AT proteasome (prosome, macropain) subunit, beta type 1 rn
RAT230_2:1398812_AT proteasome (prosome, macropain) subunit, beta type 1 rn
YEAST_2:1773552_AT 20S proteasome beta-type subunit Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5839 proteasome core complex (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4175 endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK71356 Proteasome beta subunit protein 6 [Caenorhabditis elegans] ref|NP_498806.1| Proteasome Beta Subunit family member (pbs-6) [Caenorhabditis elegans] gb|AAG50223.1| proteasome component C5 [Caenorhabditis elegans] sp|P34286|PSB1_CAEEL Proteasome subunit beta type 1 (Proteasome subunit beta 6) 1.0E-145
blast CAE56933 Hypothetical protein CBG24778 [Caenorhabditis briggsae] 1.0E-138
blast AAH58455 Proteasome (prosome, macropain) subunit, beta type 1 [Rattus norvegicus] 1.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 1.5E-42
Sequence
Target Sequence 
>C. ELEGANS:175565_S_AT
atcgagcgtcttggatactcagccagtggagccgctgaaccaatgatcattccattcttg
gattgtcaaatcgggcacgttacgctctctgaaggatatgagcgtccggaactcacattg
gacagagccatctcattgatgaaggattcgttccgtggtgctgctgagcgtgaaatctct
actggagacaaaattcacttggtcatcgctgaagccggaaagccagtcgtcgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGAGCGTCTTGGATACTCAGCCA 59 37 19 Antisense
GATACTCAGCCAGTGGAGCCGCTGA 500 423 32 Antisense
GAGCCGCTGAACCAATGATCATTCC 474 385 47 Antisense
GATTGTCAAATCGGGCACGTTACGC 252 427 79 Antisense
GCACGTTACGCTCTCTGAAGGATAT 144 315 93 Antisense
ATGAGCGTCCGGAACTCACATTGGA 120 45 116 Antisense
CACATTGGACAGAGCCATCTCATTG 463 195 132 Antisense
GATGAAGGATTCGTTCCGTGGTGCT 137 413 156 Antisense
TTCCGTGGTGCTGCTGAGCGTGAAA 111 699 169 Antisense
CACTTGGTCATCGCTGAAGCCGGAA 111 197 214 Antisense
TGAAGCCGGAAAGCCAGTCGTCGTA 297 573 228 Antisense
Sequence Source Affymetrix Proprietary Database