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[175502_at]

Description AU114995_rc /REP_DB=TREMBL Accession /GB=AU114995 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk724f10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 52      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 80      6grp_total > 62      4grp_total > 62      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 240
1 2 3 4 range
A 271
(3)
P 		168.2
(-0.5)
P 		227.5
(1.5)
P 		97.1
(-3)
P 		174
B 282
(3)
P 		172.8
(-0.8)
P 		222.1
(0.9)
P 		109.8
(-3)
P 		172
C 329.6
(3)
P 		187.9
(-0.5)
P 		262.6
(1.3)
P 		89.6
(-3)
P 		240
avr 294.2
(3) 		176.3
(-0.6) 		237.4
(1.3) 		98.8
(-3) 		195

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 170.3
(-2.1)
P 		235.9
(-0.1)
P 		333.6
(3)
P 		289.1
(1.6)
P 		160.5
(-2.4)
P 		142
(-3)
P 		192
B 167.1
(-3)
P 		275.8
(1.5)
P 		310.9
(3)
P 		263.8
(1)
P 		204.5
(-1.4)
P 		220.3
(-0.8)
P 		144
C 155.1
(-3)
P 		220.8
(0.6)
P 		225.1
(0.8)
P 		247.5
(2)
P 		258
(2.6)
P 		265.6
(3)
P 		111
avr 164.2
(-3) 		244.2
(0.8) 		289.9
(3) 		266.8
(1.9) 		207.7
(-0.9) 		209.3
(-0.8) 		126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 98.7
(3)
P 		36.5
(-1.4)
P 		59.5
(0.2)
P 		14.2
(-3)
P 		85
B 89.9
(3)
P 		42.7
(-1.3)
P 		51.5
(-0.5)
P 		23.4
(-3)
P 		67
C 109.1
(3)
P 		54
(-0.5)
P 		91.5
(1.9)
P 		15.1
(-3)
P 		94
avr 99.2
(3) 		44.4
(-1) 		67.5
(0.7) 		17.6
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 31.4
(-3)
P 		83.9
(3)
P 		72.6
(1.7)
P 		73.3
(1.8)
P 		48.4
(-1.1)
P 		34.6
(-2.6)
P 		53
B 58.1
(-3)
P 		91.8
(3)
P 		75.5
(0.1)
P 		69.9
(-0.9)
P 		83.1
(1.5)
P 		82.2
(1.3)
P 		34
C 57.5
(-1.8)
P 		67.4
(-0.3)
P 		57.3
(-1.8)
P 		49.8
(-3)
P 		83.5
(2.2)
P 		88.9
(3)
P 		39
avr 49
(-3) 		81
(3) 		68.5
(0.7) 		64.3
(-0.1) 		71.7
(1.3) 		68.6
(0.7) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175502_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21489
Sequence Type Exemplar sequence
Representative Public ID AU114995 NCBI
Target Description AU114995_rc /REP_DB=TREMBL Accession /GB=AU114995 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk724f10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069406(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069406 NCBI Caenorhabditis elegans F44D12.9 (F44D12.9) mRNA, complete cds. 11/11 None
SNAP00000020511 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10019239:10021722:1 11/11 None
GENEFINDER00000020529 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10019239:10021192:1 11/11 None
F44D12.9.1 ENSEMBL cdna:known chromosome:CEL140:IV:10018988:10021196:1 gene:F44D12.9 11/11 None
F44D12.9.2 ENSEMBL cdna:known chromosome:CEL140:IV:10019235:10021192:1 gene:F44D12.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10020649-10021189(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44D12.9
Entrez Gene 177862 Entrez gene
SwissProt Q20396 EMBL-EBI
WormBase CE32188 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5337 nucleoside transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92605 Hypothetical protein F44D12.9 [Caenorhabditis elegans] ref|NP_501807.2| F44D12.9 [Caenorhabditis elegans] 0.0
blast CAE59943 Hypothetical protein CBG03430 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 7.9E-20
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 3.8E-45
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.5E-72
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 7.9E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501807.2 10 37-59,83-105,112-134,139-161,181-200,243-265,278-300,315-332,352-374,389-411
F44D12.9.1 10 37-59,83-105,112-134,139-161,181-200,243-265,278-300,315-332,352-374,389-411
F44D12.9.2 10 37-59,83-105,112-134,139-161,181-200,243-265,278-300,315-332,352-374,389-411
SNAP00000020511 11 37-59,83-105,112-134,139-161,180-202,206-228,256-278,298-317,330-349,364-386,399-421
GENEFINDER00000020529 11 37-59,83-105,112-134,139-161,181-200,210-232,268-290,310-329,342-361,376-398,411-433
Sequence
Target Sequence 
>C. ELEGANS:175502_AT
ttcattccattttatcttttctgtaattatcgtccagatactagaagatggcctgtttgg
tttaagaatgaatggtggttcactattggatgtaccataatggcatttacttgtggttat
atgagcagtttagcgctgatttatacaccaagcaaagtgccagctagatatcagaagcta
agtggaatgcttgcatcaattttcctaatgctcggaattctcatcggagtcgccagtaca
cccattgctgcatggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCATTCCATTTTATCTTTTCTGTA 497 689 13 Antisense
TTATCTTTTCTGTAATTATCGTCCA 174 681 24 Antisense
GATACTAGAAGATGGCCTGTTTGGT 142 423 49 Antisense
ATGGTGGTTCACTATTGGATGTACC 201 49 84 Antisense
CCATAATGGCATTTACTTGTGGTTA 221 227 107 Antisense
GTTTAGCGCTGATTTATACACCAAG 607 449 140 Antisense
TACACCAAGCAAAGTGCCAGCTAGA 420 643 156 Antisense
GAATGCTTGCATCAATTTTCCTAAT 39 331 197 Antisense
CTCGGAATTCTCATCGGAGTCGCCA 679 231 223 Antisense
ATTCTCATCGGAGTCGCCAGTACAC 337 11 229 Antisense
GCCAGTACACCCATTGCTGCATGGG 134 281 244 Antisense
Sequence Source Affymetrix Proprietary Database