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[175497_at]

Description AV179662_rc /REP_DB=TREMBL Accession /GB=AV179662 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk596c12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 70      6grp_total > 18      4grp_total > 37      6grp_highest_sub > De      6grp_cgc > 01      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 421.2
(3)
P 		377.8
(1)
P 		290.6
(-3)
P 		415.5
(2.7)
P 		131
B 425.1
(3)
P 		404.5
(2.3)
P 		255.1
(-3)
P 		274.4
(-2.3)
P 		170
C 416.3
(3)
P 		318.8
(-1.5)
P 		286.8
(-3)
P 		360
(0.4)
P 		130
avr 420.9
(3) 		367
(0.7) 		277.5
(-3) 		350
(0) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 491.1
(3)
P 		366
(-0.2)
P 		254.3
(-3)
P 		329.7
(-1.1)
P 		361.9
(-0.3)
P 		255.9
(-3)
P 		237
B 420.1
(3)
P 		354.1
(1)
P 		224.6
(-3)
P 		240.2
(-2.5)
P 		270.9
(-1.6)
P 		337.3
(0.5)
P 		196
C 375.4
(3)
P 		215.5
(-3)
P 		293.8
(-0.1)
P 		307.5
(0.5)
P 		282.1
(-0.5)
P 		260.5
(-1.3)
P 		160
avr 428.9
(3) 		311.9
(-1.1) 		257.6
(-3) 		292.5
(-1.8) 		305
(-1.3) 		284.6
(-2.1) 		171
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 153.4
(3)
P 		82
(-1.6)
P 		76
(-2)
P 		60.8
(-3)
P 		93
B 135.6
(3)
P 		99.9
(0.2)
P 		59.1
(-3)
P 		58.5
(-3)
P 		77
C 137.8
(3)
P 		91.7
(-0.6)
P 		99.9
(0.1)
P 		60.7
(-3)
P 		77
avr 142.3
(3) 		91.2
(-0.7) 		78.3
(-1.7) 		60
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 90.5
(-0.2)
P 		130.2
(3)
P 		55.4
(-3)
P 		83.6
(-0.7)
P 		109.2
(1.3)
P 		62.3
(-2.4)
P 		75
B 146.2
(3)
P 		117.9
(1.1)
P 		54.5
(-3)
P 		63.6
(-2.4)
P 		110.1
(0.6)
P 		125.8
(1.7)
P 		92
C 139.1
(3)
P 		65.7
(-2.7)
P 		74.8
(-2)
P 		61.8
(-3)
P 		91.3
(-0.7)
P 		87.2
(-1)
P 		77
avr 125.3
(3) 		104.6
(1.1) 		61.6
(-3) 		69.7
(-2.2) 		103.5
(0.9) 		91.8
(-0.2) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175497_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21525
Sequence Type Exemplar sequence
Representative Public ID AV179662 NCBI
Target Description AV179662_rc /REP_DB=TREMBL Accession /GB=AV179662 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk596c12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067546(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067546 NCBI Caenorhabditis elegans Eph(F)riN family member (efn-4) (efn-4) mRNA, complete cds. 11/11 None
F56A11.3 ENSEMBL cdna:known chromosome:CEL140:IV:559238:568076:1 gene:F56A11.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000007075 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:567787-568066(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol efn-4
Ensembl F56A11.3
Entrez Gene 176882 Entrez gene
SwissProt O44516 EMBL-EBI
WormBase CE29503 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB92075 Eph(f)rin protein 4 [Caenorhabditis elegans] ref|NP_499947.1| Eph(F)riN family member (efn-4) [Caenorhabditis elegans] gb|AAL05561.1| ephrin EFN-4 [Caenorhabditis elegans] 0.0
blast CAE58470 Hypothetical protein CBG01610 [Caenorhabditis briggsae] 1.0E-112
blast CAE65681 Hypothetical protein CBG10748 [Caenorhabditis briggsae] 2.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001799 EMBL-EBI Ephrin 1.2E-74
Sequence
Target Sequence 
>C. ELEGANS:175497_AT
tactagctgtaatctatgttctatattaattttattttcttcccttttgttcccaagctc
ttcccaatttccattgtgattatcgagccatccggcttaaattcacatatcccccaaaat
caggttgttccatgcttttcccagcttcacccttccgtctctctctctttgctcaccggt
tttgaatagtatttttttgtcaaacatgtgacgggttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTAGCTGTAATCTATGTTCTATA 704 645 43 Antisense
GCTCTTCCCAATTTCCATTGTGATT 260 301 99 Antisense
CATTGTGATTATCGAGCCATCCGGC 1 217 114 Antisense
AGCCATCCGGCTTAAATTCACATAT 519 83 128 Antisense
ATCCGGCTTAAATTCACATATCCCC 569 37 132 Antisense
TAAATTCACATATCCCCCAAAATCA 599 641 140 Antisense
TATCCCCCAAAATCAGGTTGTTCCA 668 623 150 Antisense
AAATCAGGTTGTTCCATGCTTTTCC 665 117 159 Antisense
TCTCTTTGCTCACCGGTTTTGAATA 547 611 206 Antisense
GAATAGTATTTTTTTGTCAAACATG 142 327 226 Antisense
TTTTGTCAAACATGTGACGGGTTCA 421 675 237 Antisense
Sequence Source Affymetrix Proprietary Database