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[175494_at]

Description AU116547_rc /REP_DB=TREMBL Accession /GB=AU116547 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk746a11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 74      6grp_total > 64      4grp_total > 61      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 561
1 2 3 4 range
A 623.2
(3)
P 		580.7
(2.2)
P 		553.1
(1.6)
P 		317.8
(-3)
P 		305
B 806.1
(3)
P 		544
(-0.3)
P 		496.5
(-0.9)
P 		331.1
(-3)
P 		475
C 709.7
(3)
P 		508.5
(0.4)
P 		644.8
(2.2)
P 		245.1
(-3)
P 		465
avr 713
(3) 		544.4
(0.6) 		564.8
(0.9) 		298
(-3) 		415

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 403
1 2 3 4 5 6 range
A 434.7
(-3)
P 		667.7
(1)
P 		777.8
(2.8)
P 		788.4
(3)
P 		736.4
(2.1)
P 		720.4
(1.8)
P 		354
B 536.5
(-3)
P 		718.5
(0.6)
P 		837.8
(3)
P 		740.6
(1.1)
P 		721.2
(0.7)
P 		798.7
(2.2)
P 		301
C 576.3
(-1.7)
P 		713.8
(0.9)
P 		800
(2.5)
P 		609.8
(-1.1)
P 		504.9
(-3)
P 		829.1
(3)
P 		324
avr 515.8
(-3) 		700
(0.8) 		805.2
(3) 		712.9
(1.1) 		654.2
(-0.1) 		782.7
(2.5) 		289
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 226.9
(3)
P 		126.1
(-0.4)
P 		144.6
(0.3)
P 		46.5
(-3)
P 		180
B 257.1
(3)
P 		134.4
(-0.9)
P 		115
(-1.6)
P 		70.6
(-3)
P 		187
C 234.9
(3)
P 		146.2
(0.3)
P 		224.7
(2.7)
P 		41.3
(-3)
P 		194
avr 239.6
(3) 		135.6
(-0.3) 		161.4
(0.5) 		52.8
(-3) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 80.1
(-3)
P 		237.5
(3)
P 		169.4
(0.4)
P 		200
(1.6)
P 		222.2
(2.4)
P 		175.4
(0.6)
P 		157
B 186.7
(-3)
P 		239.1
(-0.2)
P 		203.4
(-2.1)
P 		196.1
(-2.5)
P 		293
(2.7)
P 		297.8
(3)
P 		111
C 213.6
(0.5)
P 		217.8
(0.7)
P 		203.6
(0.1)
P 		122.6
(-3)
P 		163.4
(-1.4)
P 		277.5
(3)
P 		155
avr 160.1
(-3) 		231.5
(1.8) 		192.1
(-0.9) 		172.9
(-2.1) 		226.2
(1.4) 		250.2
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175494_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21311
Sequence Type Exemplar sequence
Representative Public ID AU116547 NCBI
Target Description AU116547_rc /REP_DB=TREMBL Accession /GB=AU116547 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk746a11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028433(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028433 NCBI Caenorhabditis elegans ZC477.3a (ZC477.3) mRNA, complete cds. 11/11 None
ZC477.3a ENSEMBL cdna:known chromosome:CEL140:IV:7103490:7105053:1 gene:ZC477.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039647 5/11 Cross Hyb Matching Probes None
GENEFINDER00000039665 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177484 2 1 NM_001028433 175494_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7104724-7105011(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC477.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV28320 Hypothetical protein ZC477.3a [Caenorhabditis elegans] ref|NP_001023604.1| ZC477.3a [Caenorhabditis elegans] 1.0E-165
blast AAV28321 Hypothetical protein ZC477.3b [Caenorhabditis elegans] ref|NP_001023605.1| ZC477.3b [Caenorhabditis elegans] 1.0E-125
blast CAE61802 Hypothetical protein CBG05768 [Caenorhabditis briggsae] 1.0E-123
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023604.1 2 155-174,305-327
ZC477.3a 2 155-174,305-327
Sequence
Target Sequence 
>C. ELEGANS:175494_AT
tggaacgacttctcgagcagaccgaaaagaatattgaatcaaaaatgaaattgcgaacac
ttctgactgttgttgtcatttataccgctgtcgccgtaactgctccgtggctttatgccg
tattccgaggagattagccttcgacctttttactcattttaatttcgtttagatttgttt
gcattacttcaattgtattcccggtatatatattcacgtgctgccaaaagaatgttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAACGACTTCTCGAGCAGACCGA 422 545 17 Antisense
GCGAACACTTCTGACTGTTGTTGTC 460 289 69 Antisense
TTGTTGTCATTTATACCGCTGTCGC 616 705 86 Antisense
TGCTCCGTGGCTTTATGCCGTATTC 651 579 117 Antisense
GGCTTTATGCCGTATTCCGAGGAGA 155 503 125 Antisense
CGTATTCCGAGGAGATTAGCCTTCG 379 243 135 Antisense
TTACTCATTTTAATTTCGTTTAGAT 225 683 166 Antisense
GTTTAGATTTGTTTGCATTACTTCA 119 449 183 Antisense
GCATTACTTCAATTGTATTCCCGGT 51 309 197 Antisense
ATTCCCGGTATATATATTCACGTGC 304 9 213 Antisense
TCACGTGCTGCCAAAAGAATGTTAC 387 627 230 Antisense
Sequence Source Affymetrix Proprietary Database