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[175484_s_at] GTP-binding protein and M protein

Description AV199393_rc /REP_DB=TREMBL Accession /GB=AV199393 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk545c12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 43      4grp_high_8_8 > 40      6grp_highest_nonorm > 71      6grp_total > 31      4grp_total > 55      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 186
1 2 3 4 range
A 337.3
(3)
P 		200
(-1.5)
P 		228.6
(-0.6)
P 		154.5
(-3)
P 		183
B 340.4
(3)
P 		186.3
(-3)
P 		217.3
(-1.8)
P 		204.2
(-2.3)
P 		154
C 304.3
(3)
P 		210.6
(-3)
P 		216.3
(-2.6)
P 		233.4
(-1.5)
P 		94
avr 327.3
(3) 		199
(-2.9) 		220.7
(-1.9) 		197.4
(-3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 329.9
(0.8)
P 		313.2
(0.5)
P 		435.7
(3)
P 		355.5
(1.3)
P 		162.2
(-2.6)
P 		144.5
(-3)
P 		291
B 367
(1.6)
P 		421.1
(2.9)
P 		426.3
(3)
P 		284
(-0.4)
P 		186.3
(-2.7)
P 		174.1
(-3)
P 		252
C 305.7
(0.4)
P 		404.4
(3)
P 		369.6
(2.1)
P 		308.3
(0.5)
P 		177.5
(-3)
P 		211.2
(-2.1)
P 		227
avr 334.2
(1.1) 		379.6
(2.2) 		410.5
(3) 		315.9
(0.6) 		175.3
(-3) 		176.6
(-3) 		235
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 122.8
(3)
P 		43.4
(-1.8)
P 		59.8
(-0.8)
P 		22.6
(-3)
P 		100
B 108.6
(3)
P 		46
(-2.8)
P 		50.4
(-2.4)
P 		43.5
(-3)
P 		65
C 100.7
(3)
P 		60.6
(-0.9)
P 		75.4
(0.5)
P 		39.3
(-3)
P 		61
avr 110.7
(3) 		50
(-1.8) 		61.9
(-0.9) 		35.1
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 60.8
(-1)
P 		111.4
(3)
P 		94.9
(1.7)
P 		90.2
(1.3)
P 		48.9
(-1.9)
P 		35.2
(-3)
P 		76
B 127.7
(2)
P 		140.2
(3)
P 		103.5
(0.1)
P 		75.2
(-2.2)
P 		75.7
(-2.1)
P 		64.9
(-3)
P 		75
C 113.3
(2.1)
P 		123.4
(3)
P 		94.1
(0.3)
P 		62
(-2.6)
P 		57.4
(-3)
P 		70.7
(-1.8)
P 		66
avr 100.6
(0.9) 		125
(3) 		97.5
(0.6) 		75.8
(-1.3) 		60.7
(-2.7) 		56.9
(-3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175484_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22123
Sequence Type Exemplar sequence
Representative Public ID AV199393 NCBI
Target Description AV199393_rc /REP_DB=TREMBL Accession /GB=AV199393 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk545c12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077095(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077095 NCBI Caenorhabditis elegans C28G1.3 (C28G1.3) mRNA, complete cds. 11/11 None
GENEFINDER00000026789 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8829933:8835649:-1 11/11 None
C28G1.3.1 ENSEMBL cdna:known chromosome:CEL140:X:8831578:8835652:-1 gene:C28G1.3 11/11 None
C28G1.3.2 ENSEMBL cdna:known chromosome:CEL140:X:8831684:8835652:-1 gene:C28G1.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026783 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8831747-8832402(-) 100.0 100.0
Public Domain and Genome References
Gene Title GTP-binding protein and M protein
Ensembl C28G1.3
Entrez Gene 181131 Entrez gene
SwissProt Q18286 EMBL-EBI
WormBase CE37614 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251700_AT exocyst complex subunit Sec15-like family protein at
CANINE:1603461_AT similar to Exocyst complex component Sec15B cfa
CANINE_2:CFAAFFX.14182.1.S1_S_AT similar to Exocyst complex component Sec15B cfa
DROSOPHILA_2:1636866_AT dm
DROSGENOME1:150296_AT dm
CHICKEN:GGA.19594.1.S1_AT SEC15-like 2 (S. cerevisiae) gga
CHICKEN:GGAAFFX.10266.1.S1_AT SEC15-like 2 (S. cerevisiae) gga
CHICKEN:GGAAFFX.24848.1.S1_AT SEC15-like 2 (S. cerevisiae) gga
CHICKEN:GGAAFFX.24848.1.S1_S_AT SEC15-like 2 (S. cerevisiae) gga
HG-U133_PLUS_2:215417_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133A_2:215417_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133A:215417_AT SEC15-like 2 (S. cerevisiae) hs
HU35KSUBB:RC_R79319_AT SEC15-like 2 (S. cerevisiae) hs
HU35KSUBC:RC_N30328_AT SEC15-like 2 (S. cerevisiae) hs
U133_X3P:HS.44175.1.S1_3P_AT SEC15-like 2 (S. cerevisiae) hs
U133_X3P:HS.44175.0.S1_3P_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133_PLUS_2:225900_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133B:225900_AT SEC15-like 2 (S. cerevisiae) hs
HG-U95AV2:36315_I_AT SEC15-like 2 (S. cerevisiae) hs
HG-U95AV2:36316_R_AT SEC15-like 2 (S. cerevisiae) hs
HG-U95C:64065_AT SEC15-like 2 (S. cerevisiae) hs
U133_X3P:HS2.318887.1.S1_3P_X_AT SEC15-like 2 (S. cerevisiae) hs
U133_X3P:HS2.318887.1.S1_3P_S_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133_PLUS_2:1569200_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133_PLUS_2:1569201_A_AT SEC15-like 2 (S. cerevisiae) hs
HG-U133_PLUS_2:1569202_X_AT SEC15-like 2 (S. cerevisiae) hs
HG-U95C:50848_AT SEC15-like 2 (S. cerevisiae) hs
HG-U95C:66307_AT SEC15-like 2 (S. cerevisiae) hs
MG-U74BV2:114121_AT SEC15-like 2 (S. cerevisiae) mm
MOE430B:1454250_AT SEC15-like 2 (S. cerevisiae) mm
MOUSE430_2:1454250_AT SEC15-like 2 (S. cerevisiae) mm
MOE430B:1434804_AT SEC15-like 2 (S. cerevisiae) mm
MOUSE430_2:1434804_AT SEC15-like 2 (S. cerevisiae) mm
MG-U74CV2:131871_AT SEC15-like 2 (S. cerevisiae) mm
MU19KSUBC:TC36094_AT SEC15-like 2 (S. cerevisiae) mm
MOE430B:1446158_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
MOUSE430_2:1446158_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
MG-U74BV2:115980_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
MOE430B:1440622_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
MOUSE430_2:1440622_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
MU19KSUBB:TC29348_AT SEC15-like 2 (S. cerevisiae), mRNA (cDNA clone IMAGE:5370412) mm
YEAST_2:1780103_AT Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which mediates polarized targeting of vesicles to active sites of exocytosis; Sec15p associates with Sec4p and vesicles Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6904 vesicle docking during exocytosis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
145 exocyst inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAL02447 Hypothetical protein C28G1.3 [Caenorhabditis elegans] ref|NP_509496.3| C28G1.3 [Caenorhabditis elegans] sp|Q18286|SEC15_CAEEL Probable exocyst complex component Sec15 0.0
blast CAE69369 Hypothetical protein CBG15465 [Caenorhabditis briggsae] 0.0
blast T15689 hypothetical protein C28G1.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007225 EMBL-EBI Exocyst complex subunit Sec15-like 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175484_S_AT
ctcgacctcgtgatgtcatcggattggacaacgttcaatgccgaatacggaaaggatcat
gccaaatacctgagagtgaaggcatcgactgccattgttgttctagaaaaaatgattgaa
ttcgagcgaaaaagcactggattctttggaatcgccaaaggagatcgcaagaagcttctc
gacacaattgtccgtcagttgaaactcctggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCGACCTCGTGATGTCATCGGATT 370 233 30 Antisense
GATGTCATCGGATTGGACAACGTTC 526 409 41 Antisense
GACAACGTTCAATGCCGAATACGGA 39 363 56 Antisense
GGATCATGCCAAATACCTGAGAGTG 665 511 83 Antisense
ACCTGAGAGTGAAGGCATCGACTGC 157 89 97 Antisense
CATCGACTGCCATTGTTGTTCTAGA 626 211 112 Antisense
AAAGCACTGGATTCTTTGGAATCGC 222 123 160 Antisense
TTGGAATCGCCAAAGGAGATCGCAA 362 709 175 Antisense
AGGAGATCGCAAGAAGCTTCTCGAC 209 57 188 Antisense
GCTTCTCGACACAATTGTCCGTCAG 340 305 203 Antisense
TGTCCGTCAGTTGAAACTCCTGGAA 402 557 218 Antisense
Sequence Source Affymetrix Proprietary Database