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[175462_s_at] C2 domain (2 domains)

Description AV199373_rc /REP_DB=TREMBL Accession /GB=AV199373 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk545a12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 28      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 60      6grp_total > 18      4grp_total > 38      6grp_highest_sub > Cu      6grp_cgc > 01      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 215.5
(3)
P 		146.7
(-0.9)
P 		110.6
(-3)
P 		112.5
(-2.9)
P 		105
B 274.9
(3)
P 		151.7
(-0.7)
P 		76.1
(-3)
P 		138.1
(-1.1)
P 		199
C 230.1
(3)
P 		137.9
(-1.9)
P 		117.8
(-3)
P 		191.8
(1)
P 		112
avr 240.2
(3) 		145.4
(-1.1) 		101.5
(-3) 		147.5
(-1) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 581
1 2 3 4 5 6 range
A 719.4
(3)
P 		258.1
(-2.5)
P 		217.7
(-3)
P 		221
(-3)
P 		267.2
(-2.4)
P 		292
(-2.1)
P 		502
B 746.6
(3)
P 		306.3
(-1.7)
P 		188.5
(-3)
P 		233.3
(-2.5)
P 		258.6
(-2.2)
P 		320.7
(-1.6)
P 		558
C 620
(3)
P 		319.9
(-1)
P 		165.7
(-3)
P 		260.3
(-1.8)
P 		229.4
(-2.2)
P 		349.3
(-0.6)
P 		454
avr 695.3
(3) 		294.8
(-1.8) 		190.6
(-3) 		238.2
(-2.4) 		251.7
(-2.3) 		320.7
(-1.5) 		505
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 78.5
(3)
P 		31.8
(-1.5)
P 		28.9
(-1.8)
P 		16.5
(-3)
P 		62
B 87.7
(3)
P 		37.5
(-1.3)
P 		17.6
(-3)
P 		29.4
(-2)
P 		70
C 76.2
(3)
P 		39.6
(-2)
P 		41
(-1.8)
P 		32.3
(-3)
P 		44
avr 80.8
(3) 		36.3
(-1.9) 		29.2
(-2.7) 		26.1
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 132.6
(3)
P 		91.8
(0.1)
P 		47.4
(-3)
P 		56
(-2.4)
P 		80.6
(-0.7)
P 		71.1
(-1.3)
P 		85
B 259.8
(3)
P 		102
(-1.4)
P 		45.8
(-3)
P 		61.8
(-2.6)
P 		105.1
(-1.3)
P 		119.6
(-0.9)
P 		214
C 229.8
(3)
P 		97.6
(-1.2)
P 		42.2
(-3)
P 		52.3
(-2.7)
P 		74.2
(-2)
P 		116.9
(-0.6)
P 		188
avr 207.4
(3) 		97.1
(-1.1) 		45.1
(-3) 		56.7
(-2.6) 		86.6
(-1.5) 		102.5
(-0.9) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175462_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21338
Sequence Type Exemplar sequence
Representative Public ID AV199373 NCBI
Target Description AV199373_rc /REP_DB=TREMBL Accession /GB=AV199373 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk545a12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069824(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069824 NCBI Caenorhabditis elegans Gon-2 Extragenic Modifier family member (gem-4) (gem-4) mRNA, complete cds. 11/11 None
SNAP00000004566 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11745646:11748558:1 11/11 None
GENEFINDER00000004585 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11745646:11748558:1 11/11 None
T12A7.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:11745610:11748597:1 gene:T12A7.1 11/11 None
T12A7.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:11745646:11748610:1 gene:T12A7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11748307-11748593(+) 90.21 100.0
Public Domain and Genome References
Gene Title C2 domain (2 domains)
Gene Symbol gem-4
Ensembl T12A7.1
Entrez Gene 178108 Entrez gene
SwissProt Q22422 EMBL-EBI
WormBase CE27445 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1595586_AT similar to copine family member cfa
CANINE:1591533_AT similar to copine family member cfa
CANINE_2:CFA.14145.1.A1_AT similar to copine family member cfa
CANINE_2:CFA.14145.1.A1_S_AT similar to copine family member cfa
CANINE_2:CFAAFFX.16313.1.S1_S_AT similar to copine family member cfa
CANINE_2:CFAAFFX.16330.1.S1_S_AT similar to copine family member cfa
HG-U133_PLUS_2:228365_AT copine VIII hs
HG-U133B:228365_AT copine VIII hs
HU35KSUBC:RC_T25743_I_AT copine VIII hs
HU35KSUBD:RC_AA431782_AT copine VIII hs
U133_X3P:HS.98760.0.A1_3P_AT copine VIII hs
U133_X3P:HS.71818.0.A1_3P_AT copine VIII hs
U133_X3P:HS.202541.0.A1_3P_AT copine VIII hs
HG-U133_PLUS_2:241706_AT copine VIII hs
HG-U133B:241706_AT copine VIII hs
HG-U133_PLUS_2:243727_AT copine VIII hs
HG-U133B:243727_AT copine VIII hs
HG-U95C:53764_AT copine VIII hs
HG-U95C:61428_AT copine VIII hs
HG-U95D:88958_AT copine VIII hs
HG-U95B:42251_R_AT copine VIII hs
HG-U95B:46381_AT Copine VIII hs
HG-U95D:78296_AT Copine VIII hs
HG-U95E:88320_AT Copine VIII hs
HU35KSUBA:AA211147_AT Copine VIII hs
HU35KSUBB:RC_AA015782_AT Copine VIII hs
MOUSE430_2:1431146_A_AT copine VIII mm
MOE430B:1431146_A_AT copine VIII mm
MG-U74CV2:167842_R_AT copine VIII mm
MG-U74CV2:166262_F_AT copine VIII mm
MOE430B:1430520_AT copine VIII mm
MOUSE430_2:1430520_AT copine VIII mm
MOE430B:1430521_S_AT copine VIII mm
MOUSE430_2:1430521_S_AT copine VIII mm
MG-U74CV2:166078_R_AT Copine VIII (Cpne8), transcript variant 1, mRNA mm
MU19KSUBA:TC25499_AT Copine VIII (Cpne8), transcript variant 1, mRNA mm
RG-U34B:RC_AI059204_AT copine VIII (predicted) rn
RAE230B:1397050_AT copine VIII (predicted) rn
RAT230_2:1397050_AT copine VIII (predicted) rn
RAE230B:1391830_AT copine VIII (predicted) rn
RAT230_2:1391830_AT copine VIII (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA98141 Hypothetical protein T12A7.1 [Caenorhabditis elegans] ref|NP_502225.1| Gon-2 Extragenic Modifier family member (gem-4) [Caenorhabditis elegans] 0.0
blast CAE62062 Hypothetical protein CBG06080 [Caenorhabditis briggsae] 0.0
blast CAD56248 Hypothetical protein T28F3.1b [Caenorhabditis elegans] ref|NP_872104.1| T28F3.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-10
Pfam IPR000008 EMBL-EBI C2 domain 2.3E-14
Pfam IPR010734 EMBL-EBI Copine 3.3E-80
Sequence
Target Sequence 
>C. ELEGANS:175462_S_AT
gaaaatctggctcgtgaagttctttatgaagttcctgatcaattaacgggatatatgaag
cgacgaggtttcactccaaagtctcgtgacgatccatggcaaagagactctccgccagac
gaatatgatcctatcatggataatattctttcttcaactcaagcatcttcaccaactgga
gtttatccaacgatccaacacttacaagctacggcgccacccataaattcatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAATCTGGCTCGTGAAGTTCTTT 17 353 30 Antisense
GAAGTTCTTTATGAAGTTCCTGATC 430 337 45 Antisense
GAAGCGACGAGGTTTCACTCCAAAG 487 343 86 Antisense
GGTTTCACTCCAAAGTCTCGTGACG 316 503 96 Antisense
GTCTCGTGACGATCCATGGCAAAGA 160 471 110 Antisense
TGGCAAAGAGACTCTCCGCCAGACG 670 545 126 Antisense
TATTCTTTCTTCAACTCAAGCATCT 120 663 173 Antisense
TCAAGCATCTTCACCAACTGGAGTT 706 631 188 Antisense
GGAGTTTATCCAACGATCCAACACT 553 517 207 Antisense
TCCAACACTTACAAGCTACGGCGCC 411 587 223 Antisense
TACGGCGCCACCCATAAATTCATAT 645 647 239 Antisense
Sequence Source Affymetrix Proprietary Database