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[175443_at]

Description AV182344_rc /REP_DB=TREMBL Accession /GB=AV182344 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk636e12 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 07      4grp_high_8_8 > 40      6grp_total > 08      4grp_total > 47      6grp_cgc > 0      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 130.4
(3)
P 		62.1
(-3)
P 		67.5
(-2.5)
P 		81.2
(-1.3)
P 		68
B 137.2
(3)
P 		60.9
(-0.7)
P 		14.1
(-3)
A 		77.1
(0.1)
P 		123
C 151.9
(3)
P 		89.2
(-0.4)
P 		90.1
(-0.4)
P 		42.5
(-3)
P 		109
avr 139.8
(3) 		70.7
(-2) 		57.2
(-3) 		66.9
(-2.3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 110
(1.9)
P 		65.1
(-3)
A 		83.7
(-1)
P 		120.6
(3)
P 		89.1
(-0.4)
M 		73.9
(-2)
P 		56
B 128.5
(3)
P 		105.6
(0.4)
P 		97.5
(-0.5)
P 		96.4
(-0.7)
P 		76
(-3)
P 		80.1
(-2.5)
P 		53
C 174
(3)
P 		96.1
(-0.9)
P 		70.9
(-2.1)
A 		97
(-0.8)
P 		53.6
(-3)
P 		98.4
(-0.8)
P 		120
avr 137.5
(3) 		88.9
(-1.5) 		84
(-2) 		104.7
(-0) 		72.9
(-3) 		84.1
(-2) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 47.5
(3)
P 		13.5
(-2.7)
P 		17.7
(-2)
P 		11.9
(-3)
P 		36
B 43.7
(3)
P 		15
(-1.3)
P 		3.3
(-3)
A 		16.4
(-1.1)
P 		40
C 50.3
(3)
P 		25.7
(-0.4)
P 		31.4
(0.4)
P 		7.2
(-3)
P 		43
avr 47.2
(3) 		18.1
(-1.9) 		17.5
(-2) 		11.8
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 20.3
(-1.9)
P 		23.2
(-0.5)
A 		18.2
(-2.9)
P 		30.6
(3)
P 		26.9
(1.2)
M 		18
(-3)
P 		13
B 44.7
(3)
P 		35.2
(0.3)
P 		23.7
(-3)
P 		25.5
(-2.5)
P 		30.9
(-0.9)
P 		29.9
(-1.2)
P 		21
C 64.5
(3)
P 		29.3
(-1.5)
P 		18
(-2.9)
A 		19.5
(-2.7)
P 		17.3
(-3)
P 		32.9
(-1)
P 		47
avr 43.2
(3) 		29.2
(-0.6) 		20
(-3) 		25.2
(-1.7) 		25
(-1.7) 		26.9
(-1.2) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175443_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21130
Sequence Type Exemplar sequence
Representative Public ID AV182344 NCBI
Target Description AV182344_rc /REP_DB=TREMBL Accession /GB=AV182344 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk636e12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065935(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065935 NCBI Caenorhabditis elegans NeuroFibroMatosis homolog family member (nfm-1) (nfm-1) mRNA, complete cds. 11/11 A 
F42A10.2b ENSEMBL cdna:known chromosome:CEL140:III:6159819:6164455:1 gene:F42A10.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027400 3/11 Cross Hyb Matching Probes None
NM_065934 8/11 Cross Hyb Matching Probes None
F42A10.2c 3/11 Cross Hyb Matching Probes None
F42A10.2a 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175870 3 1 NM_065935 175443_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6163491-6164502(+) 100.0 100.0
Public Domain and Genome References
Ensembl F42A10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68385 Neurofibromatosis homolog protein 1, isoform b [Caenorhabditis elegans] ref|NP_498336.1| NeuroFibroMatosis homolog family member (nfm-1) [Caenorhabditis elegans] 0.0
blast AAA19073 Neurofibromatosis homolog protein 1, isoform a [Caenorhabditis elegans] ref|NP_498335.1| NeuroFibroMatosis homolog family member (nfm-1) [Caenorhabditis elegans] 0.0
blast AAW30666 Neurofibromatosis homolog protein 1, isoform c [Caenorhabditis elegans] ref|NP_001022571.1| NeuroFibroMatosis homolog family member (nfm-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 3.9E-73
Pfam IPR000798 EMBL-EBI Ezrin/radixin/moesin ERM 1.1E-42
Sequence
Target Sequence 
>C. ELEGANS:175443_AT
agacagcagtcttatgatttgggaaacactgaatggcaggaagctgacatttgaatataa
gtatgcgaggaactccacgacaacgagcacaagcttttgatggaatgtgataagaaactt
tatggccatttatgaaccgattttccttttttttcttcagtttttccccatcccgataag
agataaccccattttgtgctcattcagtttattgatttttccacacccttttgccatttt
tcaatcaataatctttcgtggcaagacaaaaacgcgtatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACAGCAGTCTTATGATTTGGGAA 342 71 21 Antisense
GATTTGGGAAACACTGAATGGCAGG 70 427 36 Antisense
GGCAGGAAGCTGACATTTGAATATA 372 535 55 Antisense
GAAACTTTATGGCCATTTATGAACC 140 357 134 Antisense
GGCCATTTATGAACCGATTTTCCTT 384 543 144 Antisense
TTTTTCCCCATCCCGATAAGAGATA 88 675 181 Antisense
GAGATAACCCCATTTTGTGCTCATT 333 397 200 Antisense
CATTTTGTGCTCATTCAGTTTATTG 373 215 210 Antisense
CACCCTTTTGCCATTTTTCAATCAA 49 199 244 Antisense
TCAATAATCTTTCGTGGCAAGACAA 578 625 265 Antisense
GTGGCAAGACAAAAACGCGTATTTT 355 491 278 Antisense
Sequence Source Affymetrix Proprietary Database