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[175407_at]

Description AV175998_rc /REP_DB=TREMBL Accession /GB=AV175998 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk499g5 : 3prime end, single read.
Cluster 6grp_total > 92      4grp_total > 17      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 21.1
(-3)
A 		44.4
(-0.8)
A 		53.2
(-0)
A 		85.7
(3)
A 		65
B 12.2
(-3)
A 		49.6
(3)
A 		18.2
(-2)
A 		40
(1.5)
A 		37
C 51.5
(0.9)
A 		21.1
(-3)
A 		31.3
(-1.7)
A 		68.1
(3)
A 		47
avr 28.3
(-3) 		38.4
(-1.3) 		34.2
(-2) 		64.6
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 9.1
(-3)
A 		25.8
(1.3)
A 		27.1
(1.6)
A 		12.4
(-2.2)
A 		32.4
(3)
A 		26
(1.4)
A 		23
B 23.9
(-3)
A 		30.6
(-2.2)
A 		35.1
(-1.7)
A 		31.1
(-2.1)
A 		73.7
(3)
P 		33.9
(-1.8)
A 		50
C 40.3
(0.2)
A 		73
(3)
A 		7.8
(-2.5)
A 		47.1
(0.8)
A 		15.6
(-1.9)
A 		2.5
(-3)
A 		71
avr 24.4
(-2) 		43.1
(3) 		23.3
(-2.3) 		30.2
(-0.5) 		40.6
(2.3) 		20.8
(-3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7.7
(-3)
A 		9.6
(-1.2)
A 		13.9
(3)
A 		12.5
(1.6)
A 		6
B 3.9
(-3)
A 		12.2
(3)
A 		4.2
(-2.8)
A 		8.5
(0.3)
A 		8
C 17
(3)
A 		6.1
(-3)
A 		10.9
(-0.4)
A 		11.5
(-0)
A 		11
avr 9.5
(-2.2) 		9.3
(-3) 		9.7
(-1.4) 		10.8
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 1.7
(-3)
A 		9.2
(2.6)
A 		5.9
(0.1)
A 		3.1
(-2)
A 		9.8
(3)
A 		6.3
(0.4)
A 		8
B 8.3
(-3)
A 		10.2
(-2.4)
A 		8.5
(-2.9)
A 		8.2
(-3)
A 		29.9
(3)
P 		12.7
(-1.8)
A 		22
C 14.9
(0.9)
A 		22.3
(3)
A 		2
(-2.7)
A 		9.5
(-0.6)
A 		5.1
(-1.8)
A 		0.8
(-3)
A 		22
avr 8.3
(-1.2) 		13.9
(2.4) 		5.5
(-3) 		6.9
(-2.1) 		14.9
(3) 		6.6
(-2.3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20630
Sequence Type Exemplar sequence
Representative Public ID AV175998 NCBI
Target Description AV175998_rc /REP_DB=TREMBL Accession /GB=AV175998 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk499g5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064100(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064100 NCBI Caenorhabditis elegans F13D12.9 (F13D12.9) mRNA, complete cds. 11/11 None
F13D12.9 ENSEMBL cdna:known chromosome:CEL140:II:11707071:11709835:-1 gene:F13D12.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11707077-11707357(-) 70.71 100.0
Public Domain and Genome References
Gene Title
Ensembl F13D12.9
Entrez Gene 174794 Entrez gene
SwissProt Q9XTE1 EMBL-EBI
WormBase CE20683 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257176_S_AT cyclopropane fatty acid synthase, putative / CPA-FA synthase, putative at
ATH1-121501:258125_S_AT cyclopropane fatty acid synthase, putative / CPA-FA synthase, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8610 lipid biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8825 cyclopropane-fatty-acyl-phospholipid synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88952 Hypothetical protein F13D12.9 [Caenorhabditis elegans] emb|CAA22134.1| Hypothetical protein F13D12.9 [Caenorhabditis elegans] ref|NP_496501.1| F13D12.9 [Caenorhabditis elegans] 0.0
blast CAE59708 Hypothetical protein CBG03140 [Caenorhabditis briggsae] 0.0
blast XP_638909 hypothetical protein DDB0185725 [Dictyostelium discoideum] gb|EAL65538.1| hypothetical protein DDB0185725 [Dictyostelium discoideum] 7.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR003333 EMBL-EBI Cyclopropane-fatty-acyl-phospholipid synthase 7.0E-37
Sequence
Target Sequence 
>C. ELEGANS:175407_AT
gcaactgttgttgaagccatatcaagtacaaaagagtcatcaccagttccaggagctgct
aaactgattggattagttccgagtgcggggacggcgcttctggtcgggaaggaaattggc
gaggtgttcgtcatggacattccctgaaaattgagttttttattggaatatttctcaaga
attctgcgagttttgcttgaaacgatacatttttgctaactgtgcgcctttaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACTGTTGTTGAAGCCATATCAA 629 319 16 Antisense
AAAAGAGTCATCACCAGTTCCAGGA 19 133 45 Antisense
TCATCACCAGTTCCAGGAGCTGCTA 198 621 52 Antisense
AGGAGCTGCTAAACTGATTGGATTA 563 55 66 Antisense
TTAGTTCCGAGTGCGGGGACGGCGC 150 561 88 Antisense
CGGCGCTTCTGGTCGGGAAGGAAAT 174 241 107 Antisense
GGAAATTGGCGAGGTGTTCGTCATG 14 527 126 Antisense
TGTTCGTCATGGACATTCCCTGAAA 258 563 140 Antisense
AAGAATTCTGCGAGTTTTGCTTGAA 425 151 192 Antisense
GATACATTTTTGCTAACTGTGCGCC 20 425 219 Antisense
TTTTGCTAACTGTGCGCCTTTAAAG 121 679 226 Antisense
Sequence Source Affymetrix Proprietary Database