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[175329_s_at] Carbonic anhydrase

Description AU113943_rc /REP_DB=TREMBL Accession /GB=AU113943 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk713d5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 31      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 81      6grp_total > 31      4grp_total > 39      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 457
1 2 3 4 range
A 447.1
(3)
P 		228.1
(-1.7)
P 		164.9
(-3)
P 		265.7
(-0.9)
P 		282
B 578.4
(3)
P 		203.8
(-1.9)
P 		121.6
(-3)
P 		246.8
(-1.4)
P 		457
C 441.1
(3)
P 		164.7
(-3)
P 		181.7
(-2.6)
P 		300.6
(-0)
P 		276
avr 488.9
(3) 		198.9
(-2.2) 		156.1
(-3) 		271
(-0.9) 		333

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 333
1 2 3 4 5 6 range
A 226.7
(0.7)
P 		247.6
(1.5)
P 		290.4
(3)
P 		180.5
(-0.9)
P 		189.6
(-0.6)
P 		122.1
(-3)
P 		168
B 273
(-0.8)
P 		323.6
(0.3)
P 		454.7
(3)
P 		184.8
(-2.6)
P 		280.2
(-0.6)
P 		166
(-3)
P 		289
C 242.6
(-0.8)
P 		328.4
(3)
P 		245.9
(-0.6)
P 		285.1
(1.1)
P 		191.6
(-3)
P 		225.1
(-1.5)
P 		137
avr 247.4
(-0.1) 		299.9
(1.9) 		330.3
(3) 		216.8
(-1.3) 		220.5
(-1.1) 		171.1
(-3) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 162.8
(3)
P 		49.5
(-2.5)
P 		43.1
(-2.8)
P 		38.9
(-3)
P 		124
B 184.5
(3)
P 		50.3
(-2.2)
P 		28.2
(-3)
P 		52.6
(-2.1)
P 		156
C 146
(3)
P 		47.3
(-3)
P 		63.3
(-2)
P 		50.7
(-2.8)
P 		99
avr 164.4
(3) 		49
(-2.8) 		44.9
(-3) 		47.4
(-2.9) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 41.8
(-1.8)
P 		88
(3)
P 		63.2
(0.4)
P 		45.8
(-1.3)
P 		57.2
(-0.2)
P 		29.7
(-3)
P 		58
B 95
(1.3)
P 		107.7
(2.4)
P 		110.4
(2.7)
P 		48.9
(-3)
P 		113.8
(3)
P 		61.9
(-1.8)
P 		65
C 89.9
(1.6)
P 		100.2
(3)
P 		62.6
(-2.3)
P 		57.3
(-3)
P 		62
(-2.3)
P 		75.4
(-0.5)
P 		43
avr 75.6
(0.1) 		98.6
(3) 		78.7
(0.5) 		50.7
(-3) 		77.7
(0.4) 		55.7
(-2.4) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175329_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20730
Sequence Type Exemplar sequence
Representative Public ID AU113943 NCBI
Target Description AU113943_rc /REP_DB=TREMBL Accession /GB=AU113943 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk713d5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063166(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063166 NCBI Caenorhabditis elegans Carbonic AnHydrase family member (cah-2) (cah-2) mRNA, complete cds. 11/11 A 
SNAP00000004650 ENSEMBL cdna:SNAP chromosome:CEL140:II:7480843:7483017:-1 11/11 None
GENEFINDER00000004655 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7480843:7485324:-1 11/11 None
D1022.8 ENSEMBL cdna:known chromosome:CEL140:II:7480739:7483035:-1 gene:D1022.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7480745-7481117(-) 100.0 100.0
Public Domain and Genome References
Gene Title Carbonic anhydrase
Gene Symbol cah-2
Ensembl D1022.8
Entrez Gene 174218 Entrez gene
SwissProt Q18932 EMBL-EBI
WormBase CE31777 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1628885_AT dm
DROSGENOME1:153025_AT dm
DROSGENOME1:144767_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6730 one-carbon compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4089 carbonate dehydratase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495567 Carbonic AnHydrase family member (cah-2) [Caenorhabditis elegans] gb|AAB93341.3| Carbonic anhydrase protein 2 [Caenorhabditis elegans] sp|Q18932|CAH2_CAEEL Putative carbonic anhydrase-like protein 2 precursor 0.0
blast CAE67520 Hypothetical protein CBG13040 [Caenorhabditis briggsae] 0.0
blast T34196 hypothetical protein D1022.8 - Caenorhabditis elegans 0.0
blast T34196 hypothetical protein D1022.8 - Caenorhabditis elegans 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 6.4E-38
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 6.4E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000004655 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:175329_S_AT
aacagtctactgtatcttcaagttgtccatcgaatgtttacgtggaaatggggtaccaag
cgaatcctggtagaaataagcggaatgattcggtttctcgaagatatgttccaacatcgg
aagttttcgaaatcgatagcattcgtcctgatgatgtttccaaagccggttcattttag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGTCTACTGTATCTTCAAGTTG 386 137 20 Antisense
ACTGTATCTTCAAGTTGTCCATCGA 277 97 28 Antisense
AGTTGTCCATCGAATGTTTACGTGG 146 63 40 Antisense
AAATGGGGTACCAAGCGAATCCTGG 148 121 65 Antisense
AGCGAATCCTGGTAGAAATAAGCGG 636 81 78 Antisense
TTTCTCGAAGATATGTTCCAACATC 580 671 113 Antisense
TGTTCCAACATCGGAAGTTTTCGAA 265 561 126 Antisense
GAAATCGATAGCATTCGTCCTGATG 707 359 148 Antisense
TAGCATTCGTCCTGATGATGTTTCC 55 651 156 Antisense
CTGATGATGTTTCCAAAGCCGGTTC 601 237 167 Antisense
TGTTTCCAAAGCCGGTTCATTTTAG 373 549 174 Antisense
Sequence Source Affymetrix Proprietary Database