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[175314_s_at]

Description AU114544_rc /REP_DB=TREMBL Accession /GB=AU114544 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk720e2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_high_8_8 > 43      6grp_highest_nonorm > 70      6grp_total > 34      4grp_total > 52      6grp_highest_sub > 01_02_03      6grp_cgc > 44      6grp_high1_sub > 03      6grp_cgc_sub > 02      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 316.3
(3)
P 		313.8
(2.9)
P 		227.8
(0.7)
P 		83.1
(-3)
A 		233
B 341.6
(3)
P 		271.3
(1.4)
P 		288.5
(1.8)
P 		70
(-3)
P 		272
C 309.4
(3)
P 		271.5
(2.1)
P 		200.9
(0.4)
P 		59.3
(-3)
P 		250
avr 322.4
(3) 		285.5
(2.1) 		239.1
(1) 		70.8
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 338
1 2 3 4 5 6 range
A 305.1
(0.1)
P 		424.9
(2.3)
P 		466.4
(3)
P 		245.4
(-0.9)
P 		128.8
(-3)
P 		281.5
(-0.3)
P 		338
B 339.3
(1.1)
P 		408.3
(2.6)
P 		429
(3)
P 		142.1
(-3)
P 		272.9
(-0.3)
P 		257.5
(-0.6)
P 		287
C 374.4
(2.6)
P 		386
(3)
P 		349.2
(1.7)
P 		280.6
(-0.6)
P 		209.8
(-3)
P 		219.1
(-2.7)
P 		176
avr 339.6
(0.9) 		406.4
(2.8) 		414.9
(3) 		222.7
(-2.5) 		203.8
(-3) 		252.7
(-1.6) 		211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 115.2
(3)
P 		68.1
(0.3)
P 		59.6
(-0.2)
P 		12.2
(-3)
A 		103
B 108.9
(3)
P 		67
(0.3)
P 		66.9
(0.3)
P 		14.9
(-3)
P 		94
C 102.4
(3)
P 		78.1
(1.4)
P 		70
(0.9)
P 		10
(-3)
P 		92
avr 108.8
(3) 		71.1
(0.7) 		65.5
(0.3) 		12.4
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 56.2
(-2.1)
P 		151.1
(3)
P 		101.6
(0.4)
P 		62.2
(-1.8)
P 		38.9
(-3)
P 		68.5
(-1.4)
P 		112
B 118.1
(1.9)
P 		135.9
(3)
P 		104.2
(1.1)
P 		37.6
(-3)
P 		110.9
(1.5)
P 		96
(0.6)
P 		98
C 138.8
(3)
P 		117.8
(1.5)
P 		88.9
(-0.6)
P 		56.4
(-3)
P 		67.9
(-2.2)
P 		73.3
(-1.8)
P 		82
avr 104.4
(0.8) 		134.9
(3) 		98.2
(0.3) 		52.1
(-3) 		72.6
(-1.5) 		79.3
(-1) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175314_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21205
Sequence Type Exemplar sequence
Representative Public ID AU114544 NCBI
Target Description AU114544_rc /REP_DB=TREMBL Accession /GB=AU114544 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk720e2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066660(11), NM_066661(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066660 NCBI Caenorhabditis elegans Cell-death-Related Nuclease family member (crn-6) (crn-6) mRNA, complete cds. 11/11 A 
NM_066661 NCBI Caenorhabditis elegans Cell-death-Related Nuclease family member (crn-6) (crn-6) mRNA, complete cds. 11/11 A 
SNAP00000032563 ENSEMBL cdna:SNAP chromosome:CEL140:III:9367169:9369334:1 11/11 None
GENEFINDER00000032566 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9367169:9369334:1 11/11 None
K04H4.6a ENSEMBL cdna:known chromosome:CEL140:III:9367169:9369334:1 gene:K04H4.6 11/11 A 
K04H4.6b ENSEMBL cdna:known chromosome:CEL140:III:9367169:9370219:1 gene:K04H4.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9369042-9370200(+) 98.21 98.21
Public Domain and Genome References
Ensembl K04H4.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA81586 Hypothetical protein K04H4.6b [Caenorhabditis elegans] emb|CAD45582.1| Hypothetical protein K04H4.6b [Caenorhabditis elegans] ref|NP_499061.2| Cell-death-Related Nuclease family member (crn-6) [Caenorhabditis elegans] sp|P34508|CRN6_CAEEL Cell death-related nuclease 6 precursor 0.0
blast CAB76843 Hypothetical protein K04H4.6a [Caenorhabditis elegans] ref|NP_499062.1| Cell-death-Related Nuclease family member (crn-6) [Caenorhabditis elegans] gb|AAP57302.1| cell death-related nuclease 6 [Caenorhabditis elegans] 0.0
blast AAF43008 DNAse II homolog K04H4.6 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004947 EMBL-EBI Deoxyribonuclease II 4.7E-86
Sequence
Target Sequence 
>C. ELEGANS:175314_S_AT
gatatcaatcgtcagagtcatcaaatccatcgtggcggtggaacaatgtgcatctacgat
caagaaacatacttccaattcgcaaatatcatcagtgaaacagtgccttgcacaaaagca
acggctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATCAATCGTCAGAGTCATCAAA 108 425 84 Antisense
CAGAGTCATCAAATCCATCGTGGCG 147 207 96 Antisense
GTCATCAAATCCATCGTGGCGGTGG 108 467 100 Antisense
CCATCGTGGCGGTGGAACAATGTGC 421 269 110 Antisense
GGCGGTGGAACAATGTGCATCTACG 396 541 117 Antisense
GGAACAATGTGCATCTACGATCAAG 570 527 123 Antisense
GTGCATCTACGATCAAGAAACATAC 15 481 131 Antisense
AGAAACATACTTCCAATTCGCAAAT 27 73 146 Antisense
TTCCAATTCGCAAATATCATCAGTG 183 701 156 Antisense
ATCATCAGTGAAACAGTGCCTTGCA 490 29 171 Antisense
GTGCCTTGCACAAAAGCAACGGCTG 285 477 186 Antisense
Sequence Source Affymetrix Proprietary Database