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[175312_at]

Description AU115690_rc /REP_DB=TREMBL Accession /GB=AU115690 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk735f2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 01      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 90      6grp_total > 23      4grp_total > 38      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 217.8
(3)
P 		174.5
(-1.3)
P 		157.2
(-3)
P 		181.8
(-0.6)
P 		61
B 207.9
(3)
P 		199
(1.3)
P 		177.3
(-3)
P 		198.8
(1.2)
P 		31
C 244.9
(3)
P 		203.5
(-0.9)
P 		181
(-3)
P 		186.7
(-2.5)
P 		64
avr 223.5
(3) 		192.3
(-0.6) 		171.8
(-3) 		189.1
(-1) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 206.7
(2.1)
P 		221.4
(3)
P 		202.9
(1.8)
P 		162.9
(-0.7)
P 		139.4
(-2.2)
P 		125.9
(-3)
P 		96
B 225.3
(3)
P 		162.7
(-1.1)
P 		132.9
(-3)
P 		188.5
(0.6)
P 		152.4
(-1.7)
P 		137
(-2.7)
P 		92
C 169.5
(-0.3)
P 		134.3
(-2.5)
P 		221.4
(3)
P 		147.6
(-1.7)
P 		127.1
(-3)
P 		143.5
(-2)
P 		94
avr 200.5
(3) 		172.8
(0.4) 		185.7
(1.6) 		166.3
(-0.2) 		139.6
(-2.6) 		135.5
(-3) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 79.3
(3)
P 		37.9
(-1.7)
P 		41.1
(-1.3)
P 		26.6
(-3)
P 		53
B 66.3
(3)
P 		49.1
(-1.1)
P 		41.1
(-3)
P 		42.4
(-2.7)
P 		25
C 81.1
(3)
P 		58.5
(0.3)
P 		63.1
(0.8)
P 		31.5
(-3)
P 		50
avr 75.6
(3) 		48.5
(-0.9) 		48.4
(-0.9) 		33.5
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 38.1
(-2.1)
P 		78.8
(3)
P 		44.2
(-1.3)
P 		41.3
(-1.7)
P 		42.1
(-1.6)
P 		30.7
(-3)
P 		48
B 78.4
(3)
P 		54.1
(-0.2)
P 		32.3
(-3)
P 		49.9
(-0.7)
P 		61.9
(0.9)
P 		51.1
(-0.6)
P 		46
C 62.8
(3)
P 		41
(-1)
P 		56.4
(1.8)
P 		29.7
(-3)
P 		41.1
(-0.9)
P 		48.1
(0.3)
P 		33
avr 59.8
(3) 		58
(2.4) 		44.3
(-1.8) 		40.3
(-3) 		48.4
(-0.5) 		43.3
(-2.1) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22059
Sequence Type Exemplar sequence
Representative Public ID AU115690 NCBI
Target Description AU115690_rc /REP_DB=TREMBL Accession /GB=AU115690 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk735f2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029309(11), NM_001029310(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029309 NCBI Caenorhabditis elegans Choline Kinase A family member (cka-2) (cka-2) mRNA, complete cds. 11/11 None
NM_001029310 NCBI Caenorhabditis elegans Choline Kinase A family member (cka-2) (cka-2) mRNA, complete cds. 9/11 None
SNAP00000014556 ENSEMBL cdna:SNAP chromosome:CEL140:X:4289463:4291335:1 9/11 None
GENEFINDER00000014571 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4289463:4291335:1 9/11 None
C52B9.1 ENSEMBL cdna:known chromosome:CEL140:X:4286711:4291447:1 gene:C52B9.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4291066-4291398(+) 100.0 100.0
Public Domain and Genome References
Ensembl C52B9.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024480 Choline Kinase A family member (cka-2) [Caenorhabditis elegans] gb|AAK39220.1| Choline kinase a protein 2, isoform a [Caenorhabditis elegans] pdb|1NW1|B Chain B, Crystal Structure Of Choline Kinase pdb|1NW1|A Chain A, Crystal Structure Of Choline Kinase 0.0
blast CAE68555 Hypothetical protein CBG14388 [Caenorhabditis briggsae] 0.0
blast NP_001024481 Choline Kinase A family member (cka-2) [Caenorhabditis elegans] gb|AAX88835.1| Choline kinase a protein 2, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175312_AT
tcatttcttttggggtgtgtggggactgttgcaagtcgaactttccccagttggattcgg
atttgcggactacgggagagatcgtctgtcgctgtacttcaaacataaacaacttctcaa
aaaccttgcaagtcatcaataagatcttcttttaatctcaattttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATTTCTTTTGGGGTGTGTGGGGA 239 617 95 Antisense
TTTGGGGTGTGTGGGGACTGTTGCA 649 665 103 Antisense
GACTGTTGCAAGTCGAACTTTCCCC 343 375 118 Antisense
GTCGAACTTTCCCCAGTTGGATTCG 164 473 129 Antisense
GAACTTTCCCCAGTTGGATTCGGAT 98 339 132 Antisense
CCCCAGTTGGATTCGGATTTGCGGA 405 257 139 Antisense
GGATTCGGATTTGCGGACTACGGGA 102 407 147 Antisense
TCGGATTTGCGGACTACGGGAGAGA 546 603 151 Antisense
GTACTTCAAACATAAACAACTTCTC 669 453 188 Antisense
GCAAGTCATCAATAAGATCTTCTTT 348 321 222 Antisense
GATCTTCTTTTAATCTCAATTTTTA 108 421 237 Antisense
Sequence Source Affymetrix Proprietary Database