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[175299_at]

Description AU115222_rc /REP_DB=TREMBL Accession /GB=AU115222 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk729d3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 86      4grp_highest_8_8 > 27      4grp_high_8_8 > 57      6grp_highest_nonorm > 94      6grp_total > 77      4grp_total > 63      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 279
1 2 3 4 range
A 456.4
(-1.4)
P 		492.8
(1)
P 		523.6
(3)
P 		432.8
(-3)
P 		91
B 519.2
(-0.1)
P 		535.6
(1.8)
P 		545.8
(3)
P 		495
(-3)
P 		51
C 664.5
(2)
P 		434.4
(-3)
P 		712
(3)
P 		448.3
(-2.7)
P 		278
avr 546.7
(0.9) 		487.6
(-1.7) 		593.8
(3) 		458.7
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 757
1 2 3 4 5 6 range
A 289.4
(-3)
P 		463
(-0.9)
P 		514
(-0.3)
P 		493.7
(-0.5)
P 		617.6
(1)
P 		785.9
(3)
P 		497
B 474.8
(-2.7)
P 		444.1
(-3)
P 		619
(-1.3)
P 		447
(-3)
P 		573.1
(-1.7)
P 		1046.2
(3)
P 		602
C 484.5
(-2.8)
P 		465
(-3)
P 		538.8
(-2.2)
P 		534.2
(-2.2)
P 		576.1
(-1.8)
P 		1003
(3)
P 		538
avr 416.2
(-3) 		457.4
(-2.5) 		557.3
(-1.4) 		491.6
(-2.1) 		588.9
(-1) 		945
(3) 		529
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 166.2
(3)
P 		107
(-0.5)
P 		136.9
(1.3)
P 		63.3
(-3)
P 		103
B 165.6
(3)
P 		132.3
(-0.3)
P 		126.5
(-0.9)
P 		105.5
(-3)
P 		60
C 220
(2)
P 		124.9
(-1.3)
P 		248.2
(3)
P 		75.6
(-3)
P 		173
avr 183.9
(3) 		121.4
(-0.7) 		170.5
(2.2) 		81.5
(-3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 337
1 2 3 4 5 6 range
A 53.3
(-3)
P 		164.7
(1.8)
P 		111.9
(-0.5)
P 		125.2
(0.1)
P 		186.4
(2.8)
P 		191.3
(3)
P 		138
B 165.2
(-2)
P 		147.8
(-2.4)
P 		150.3
(-2.3)
P 		118.4
(-3)
P 		232.8
(-0.5)
P 		390.1
(3)
P 		272
C 179.6
(-1.1)
P 		141.9
(-2.1)
P 		137.1
(-2.2)
P 		107.4
(-3)
P 		186.4
(-0.9)
P 		335.8
(3)
P 		228
avr 132.7
(-2.5) 		151.5
(-1.9) 		133.1
(-2.5) 		117
(-3) 		201.9
(-0.3) 		305.7
(3) 		189
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175299_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20495
Sequence Type Exemplar sequence
Representative Public ID AU115222 NCBI
Target Description AU115222_rc /REP_DB=TREMBL Accession /GB=AU115222 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk729d3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063305(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063305 NCBI Caenorhabditis elegans F10B5.8 (F10B5.8) mRNA, complete cds. 11/11 A 
SNAP00000014811 ENSEMBL cdna:SNAP chromosome:CEL140:II:8141207:8143218:-1 11/11 None
GENEFINDER00000014824 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8141207:8143218:-1 11/11 None
F10B5.8 ENSEMBL cdna:known chromosome:CEL140:II:8141142:8143318:-1 gene:F10B5.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8141176-8141485(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F10B5.8
Entrez Gene 174310 Entrez gene
SwissProt Q9U3K2 EMBL-EBI
WormBase CE32859 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586630_AT similar to related to CPSF subunits 68 kDa isoform 1 cfa
CANINE_2:CFA.11512.1.A1_AT similar to related to CPSF subunits 68 kDa isoform 1 cfa
CANINE_2:CFAAFFX.29444.1.S1_S_AT similar to related to CPSF subunits 68 kDa isoform 1 cfa
DROSOPHILA_2:1626880_AT dm
DROSGENOME1:153639_AT dm
CHICKEN:GGA.13403.1.S1_AT cleavage and polyadenylation specific factor 3-like gga
CHICKEN:GGAAFFX.12917.1.S1_S_AT cleavage and polyadenylation specific factor 3-like gga
HG-U133_PLUS_2:224312_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133B:224312_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133_PLUS_2:233625_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133B:233625_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133A_2:217994_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133A:217994_X_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133_PLUS_2:217994_X_AT cleavage and polyadenylation specific factor 3-like hs
HU35KSUBA:RC_AA477561_AT cleavage and polyadenylation specific factor 3-like hs
HU35KSUBA:W17119_AT cleavage and polyadenylation specific factor 3-like hs
U133_X3P:G8923511_3P_X_AT cleavage and polyadenylation specific factor 3-like hs
U133_X3P:G12653778_3P_X_AT cleavage and polyadenylation specific factor 3-like hs
U133_X3P:HS.6449.4.A1_3P_A_AT cleavage and polyadenylation specific factor 3-like hs
U133_X3P:HS.6449.3.S1_3P_X_AT cleavage and polyadenylation specific factor 3-like hs
U133_X3P:HS.134740.0.S1_3P_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133_PLUS_2:232437_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133B:232437_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133_PLUS_2:233563_S_AT cleavage and polyadenylation specific factor 3-like hs
HG-U133B:233563_S_AT cleavage and polyadenylation specific factor 3-like hs
HG-U95B:43402_AT cleavage and polyadenylation specific factor 3-like hs
HG-U95B:45401_S_AT cleavage and polyadenylation specific factor 3-like hs
HG-U95C:50164_AT cleavage and polyadenylation specific factor 3-like hs
HG-U95D:78680_AT cleavage and polyadenylation specific factor 3-like hs
MG-U74AV2:94053_AT cleavage and polyadenylation specific factor 3-like mm
MOE430A:1423964_AT cleavage and polyadenylation specific factor 3-like mm
MOUSE430A_2:1423964_AT cleavage and polyadenylation specific factor 3-like mm
MOUSE430_2:1423964_AT cleavage and polyadenylation specific factor 3-like mm
MG-U74CV2:129121_AT Cleavage and polyadenylation specific factor 3-like (Cpsf3l), mRNA mm
MU19KSUBC:TC36064_AT Cleavage and polyadenylation specific factor 3-like (Cpsf3l), mRNA mm
MU19KSUBC:TC39803_AT Cleavage and polyadenylation specific factor 3-like (Cpsf3l), mRNA mm
RG-U34B:RC_AI029007_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAE230B:1390967_A_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAT230_2:1390967_A_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAE230A:1377316_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAT230_2:1377316_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAE230B:1395732_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RAT230_2:1395732_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
RG-U34A:RC_AI639203_AT Similar to RIKEN cDNA 2410006F12 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB54223 Hypothetical protein F10B5.8 [Caenorhabditis elegans] ref|NP_495706.2| F10B5.8 [Caenorhabditis elegans] 0.0
blast CAE57734 Hypothetical protein CBG00745 [Caenorhabditis briggsae] 0.0
blast CAG32473 hypothetical protein [Gallus gallus] ref|NP_001012854.1| cleavage and polyadenylation specific factor 3-like [Gallus gallus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 8.3E-17
Sequence
Target Sequence 
>C. ELEGANS:175299_AT
actctctcattgtcagaaatcatcaaaggcaaaaaagtgaattggaaggaattatcaaac
gagcttctactctatgatccacacttgcaactaaaagacgacggcattgaaatgtttgat
ggagaaatcactattttagcaaacaattctgatgaaacagagcttgaactgatctgggac
gaatgccgtgaacagtggttcaaggttatccataaagccatcactgatctcatttcaccc
gaatcctccccggtccctcctgctatctctgttaaagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCTCTCATTGTCAGAAATCATCA 550 99 13 Antisense
GGAATTATCAAACGAGCTTCTACTC 479 533 60 Antisense
CGAGCTTCTACTCTATGATCCACAC 420 251 72 Antisense
TACTCTATGATCCACACTTGCAACT 659 645 80 Antisense
TGCAACTAAAAGACGACGGCATTGA 21 575 98 Antisense
GAGCTTGAACTGATCTGGGACGAAT 128 389 172 Antisense
GGACGAATGCCGTGAACAGTGGTTC 706 521 189 Antisense
GAACAGTGGTTCAAGGTTATCCATA 617 351 202 Antisense
AGGTTATCCATAAAGCCATCACTGA 424 57 215 Antisense
AAAGCCATCACTGATCTCATTTCAC 431 125 226 Antisense
CCCTCCTGCTATCTCTGTTAAAGCA 125 261 267 Antisense
Sequence Source Affymetrix Proprietary Database