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[175282_at]

Description AV177572_rc /REP_DB=TREMBL Accession /GB=AV177572 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk530e7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 82      6grp_total > 59      4grp_total > 55      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 428.3
(3)
P 		325.8
(-3)
P 		335.7
(-2.4)
P 		325
(-3)
P 		103
B 524.1
(3)
P 		347.3
(-1.3)
P 		331.7
(-1.7)
P 		277.1
(-3)
P 		247
C 457.6
(3)
P 		329.5
(-2.1)
P 		350.8
(-1.3)
P 		307.9
(-3)
P 		150
avr 470
(3) 		334.2
(-1.9) 		339.4
(-1.7) 		303.3
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 344.2
(-1.2)
P 		317
(-1.9)
P 		272.5
(-3)
P 		332.3
(-1.5)
P 		372.7
(-0.5)
P 		516.7
(3)
P 		244
B 400.2
(3)
P 		296
(-3)
P 		300.4
(-2.7)
P 		311.9
(-2.1)
P 		334.1
(-0.8)
P 		353.3
(0.3)
P 		104
C 376.9
(2)
P 		309
(-0.9)
P 		350.7
(0.9)
P 		260
(-3)
P 		387.7
(2.4)
P 		400.8
(3)
P 		141
avr 373.8
(0.6) 		307.3
(-2.7) 		307.9
(-2.7) 		301.4
(-3) 		364.8
(0.1) 		423.6
(3) 		122
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 155.9
(3)
P 		70.7
(-1.7)
P 		87.8
(-0.8)
P 		47.6
(-3)
P 		108
B 167.1
(3)
P 		85.8
(-1.5)
P 		76.9
(-2)
P 		59.1
(-3)
P 		108
C 151.5
(3)
P 		94.7
(-0.4)
P 		122.3
(1.2)
P 		51.9
(-3)
P 		100
avr 158.2
(3) 		83.7
(-1.2) 		95.7
(-0.6) 		52.9
(-3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 63.5
(-2.6)
P 		112.8
(1.8)
P 		59.3
(-3)
P 		84.3
(-0.7)
P 		112.5
(1.8)
P 		125.8
(3)
P 		67
B 139.2
(3)
P 		98.5
(-0.7)
P 		72.9
(-3)
P 		82.6
(-2.1)
P 		135.7
(2.7)
P 		131.8
(2.3)
P 		66
C 139.7
(3)
P 		94.3
(-0.1)
P 		89.3
(-0.5)
P 		52.3
(-3)
P 		125.4
(2)
P 		134.2
(2.6)
P 		87
avr 114.1
(1.3) 		101.9
(0) 		73.8
(-2.9) 		73.1
(-3) 		124.5
(2.4) 		130.6
(3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175282_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20058
Sequence Type Exemplar sequence
Representative Public ID AV177572 NCBI
Target Description AV177572_rc /REP_DB=TREMBL Accession /GB=AV177572 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk530e7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058484(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058484 NCBI Caenorhabditis elegans Y71G12B.12a (Y71G12B.12) mRNA, complete cds. 11/11 None
GENEFINDER00000001816 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1709588:1715396:1 9/11 None
Y71G12B.12a ENSEMBL cdna:known chromosome:CEL140:I:1709588:1715562:1 gene:Y71G12B.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001785 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171735 2 1 NM_058484 175282_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1715212-1715486(+) 97.86 97.86
Public Domain and Genome References
Ensembl Y71G12B.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_490885 Y71G12B.12a [Caenorhabditis elegans] 0.0
blast ABA00156 Hypothetical protein Y71G12B.12a [Caenorhabditis elegans] 1.0E-112
blast NP_871858 Y71G12B.12b [Caenorhabditis elegans] 4.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR007239 EMBL-EBI Autophagy protein Apg5 3.7E-14
Pfam IPR007239 EMBL-EBI Autophagy protein Apg5 4.4E-7
Pfam IPR007239 EMBL-EBI Autophagy protein Apg5 4.4E-7
Sequence
Target Sequence 
>C. ELEGANS:175282_AT
agctgtctcagatgttctatcatcatcatcatcatcctcaacggattctcaatcggaaca
cccaccacgtctgatctcgcacggaatcgacattccccatcacaccccattgattttcgc
cgccaaaaacttgtcctatccggataactttattcacgttgtcctcctcctcgtcgtccc
ttaactcttcatcactgcatcttttttattgattttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTGTCTCAGATGTTCTATCATCA 639 79 13 Antisense
TCTCAGATGTTCTATCATCATCATC 459 613 18 Antisense
CATCATCCTCAACGGATTCTCAATC 538 213 42 Antisense
AACGGATTCTCAATCGGAACACCCA 622 141 52 Antisense
ACGTCTGATCTCGCACGGAATCGAC 453 93 79 Antisense
TCTCGCACGGAATCGACATTCCCCA 697 609 87 Antisense
GCCGCCAAAAACTTGTCCTATCCGG 641 283 131 Antisense
ACTTGTCCTATCCGGATAACTTTAT 555 103 141 Antisense
ATAACTTTATTCACGTTGTCCTCCT 256 3 156 Antisense
CTCTTCATCACTGCATCTTTTTTAT 90 231 197 Antisense
CACTGCATCTTTTTTATTGATTTTT 33 199 205 Antisense
Sequence Source Affymetrix Proprietary Database