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[175245_at] flavin-containing monoxygenase

Description AU113264_rc /REP_DB=TREMBL Accession /GB=AU113264 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk705b2 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 82      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 64      6grp_total > 73      4grp_total > 57      6grp_highest_sub > 04_05      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1470
1 2 3 4 range
A 355.5
(-2.7)
P 		1361.9
(1.6)
P 		1678.8
(3)
P 		282
(-3)
P 		1397
B 335.3
(-2.3)
P 		969.7
(1.2)
P 		1294.6
(3)
P 		217.5
(-3)
P 		1077
C 307.7
(-2.7)
P 		1018.1
(0.2)
P 		1687.8
(3)
P 		234.2
(-3)
P 		1454
avr 332.8
(-2.6) 		1116.6
(1) 		1553.7
(3) 		244.6
(-3) 		1309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 614
1 2 3 4 5 6 range
A 534.7
(-3)
P 		596.5
(-2.3)
P 		1024
(2.8)
P 		925.7
(1.7)
P 		1038.3
(3)
P 		763.8
(-0.3)
P 		504
B 579.5
(-3)
P 		624.4
(-2.4)
P 		730.9
(-1.1)
P 		969.6
(1.9)
P 		1058.6
(3)
P 		858
(0.5)
P 		479
C 662.1
(-2.4)
P 		608
(-3)
P 		1036.4
(1.8)
P 		1031.9
(1.7)
P 		1148.8
(3)
P 		663.6
(-2.4)
P 		541
avr 592.1
(-3) 		609.6
(-2.8) 		930.4
(1.1) 		975.7
(1.7) 		1081.9
(3) 		761.8
(-0.9) 		490
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 549
1 2 3 4 range
A 129.4
(-1.7)
P 		295.6
(0.8)
P 		438.9
(3)
P 		41.3
(-3)
P 		398
B 106.9
(-1.6)
P 		239.5
(1.6)
P 		300
(3)
P 		46.3
(-3)
P 		254
C 101.8
(-2.3)
P 		292.7
(-0.2)
P 		588.3
(3)
P 		39.5
(-3)
P 		549
avr 112.7
(-1.9) 		275.9
(0.5) 		442.4
(3) 		42.4
(-3) 		400

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 98.6
(-3)
P 		212.2
(0.2)
P 		223
(0.5)
P 		234.8
(0.8)
P 		313.3
(3)
P 		186
(-0.6)
P 		215
B 201.6
(-2.4)
P 		207.8
(-2.3)
P 		177.5
(-3)
P 		256.8
(-1.1)
P 		430.1
(3)
P 		320
(0.4)
P 		253
C 245.4
(-1.1)
P 		185.5
(-3)
P 		263.8
(-0.5)
P 		207.5
(-2.3)
P 		371.7
(3)
P 		222.1
(-1.8)
P 		186
avr 181.9
(-3) 		201.8
(-2.4) 		221.4
(-1.8) 		233
(-1.4) 		371.7
(3) 		242.7
(-1.1) 		190
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175245_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21910
Sequence Type Exemplar sequence
Representative Public ID AU113264 NCBI
Target Description AU113264_rc /REP_DB=TREMBL Accession /GB=AU113264 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk705b2 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073969(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073969 NCBI Caenorhabditis elegans Flavin-containing MonoOxygenase family member (fmo-4) (fmo-4) mRNA, complete cds. 11/11 A 
SNAP00000012438 ENSEMBL cdna:SNAP chromosome:CEL140:V:13606172:13609286:-1 9/11 None
F53F4.5.1 ENSEMBL cdna:known chromosome:CEL140:V:13605887:13609293:-1 gene:F53F4.5 11/11 A 
F53F4.5.2 ENSEMBL cdna:known chromosome:CEL140:V:13606018:13609295:-1 gene:F53F4.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13606078-13606366(-) 100.0 100.0
Public Domain and Genome References
Gene Title flavin-containing monoxygenase
Gene Symbol fmo-4
Ensembl F53F4.5
Entrez Gene 179845 Entrez gene
SwissProt Q20730 EMBL-EBI
WormBase CE30676 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265122_AT flavin-containing monooxygenase family protein / FMO family protein at
ATGENOME1:12944_AT flavin-containing monooxygenase family protein / FMO family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5792 microsome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
4499 dimethylaniline monooxygenase (N-oxide-forming) activity inferred from electronic annotation QuickGO AmiGO
15036 disulfide oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01214 Hypothetical protein F53F4.5 [Caenorhabditis elegans] ref|NP_506370.2| Flavin-containing MonoOxygenase family member (fmo-4) [Caenorhabditis elegans] emb|CAE46113.1| flavin monooxygenase [Caenorhabditis elegans] 0.0
blast CAE66281 Hypothetical protein CBG11527 [Caenorhabditis briggsae] 0.0
blast AAH79705 MGC81930 protein [Xenopus laevis] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR000960 EMBL-EBI Flavin-containing monooxygenase FMO 4.4E-40
Pfam IPR000960 EMBL-EBI Flavin-containing monooxygenase FMO 1.0E-93
Pfam IPR000960 EMBL-EBI Flavin-containing monooxygenase FMO 1.4E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506370.2 2 512-531,538-560
F53F4.5.1 2 512-531,538-560
F53F4.5.2 2 512-531,538-560
SNAP00000012438 2 530-549,556-578
Sequence
Target Sequence 
>C. ELEGANS:175245_AT
atgcgactcaaaaagtgattgccacaaacatttcaattctattctgctccggtcttcttc
tatactgctccacaacatcaacacttcctccagctgtttatttcttctcattcttccttt
tctttactctttacggtgcaacactaatgtggtttgacctacaatatgacatgaccacaa
tcttctagattcaatttaattgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCGACTCAAAAAGTGATTGCCAC 594 41 20 Antisense
AAAGTGATTGCCACAAACATTTCAA 20 123 31 Antisense
ACAAACATTTCAATTCTATTCTGCT 127 113 43 Antisense
TTCTATACTGCTCCACAACATCAAC 210 691 77 Antisense
TCCAGCTGTTTATTTCTTCTCATTC 16 591 108 Antisense
TTTCTTTACTCTTTACGGTGCAACA 583 671 138 Antisense
TTTACTCTTTACGGTGCAACACTAA 167 667 142 Antisense
GCAACACTAATGTGGTTTGACCTAC 318 317 157 Antisense
TTTGACCTACAATATGACATGACCA 77 661 172 Antisense
ATGACATGACCACAATCTTCTAGAT 357 45 185 Antisense
AATCTTCTAGATTCAATTTAATTGT 26 171 198 Antisense
Sequence Source Affymetrix Proprietary Database