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[175238_s_at]

Description AU114285_rc /REP_DB=TREMBL Accession /GB=AU114285 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk717c7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 32      6grp_total > 22      4grp_total > 62      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > De      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1295
1 2 3 4 range
A 1753.4
(3)
P 		1118.4
(0.1)
P 		1368.2
(1.2)
P 		458
(-3)
P 		1295
B 1752.5
(3)
P 		1134.5
(-0.3)
P 		1409.5
(1.2)
P 		637.9
(-3)
P 		1115
C 1741.6
(3)
P 		1049.4
(-0.6)
P 		1486.7
(1.7)
P 		592.8
(-3)
P 		1149
avr 1749.2
(3) 		1100.8
(-0.3) 		1421.5
(1.3) 		562.9
(-3) 		1186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1082
1 2 3 4 5 6 range
A 919.6
(0.1)
P 		1336.4
(3)
P 		1287.5
(2.7)
P 		847.6
(-0.4)
P 		590.8
(-2.1)
P 		464.1
(-3)
P 		872
B 1332.3
(1.7)
P 		1546.1
(3)
P 		1187.7
(0.9)
P 		805.9
(-1.3)
P 		601.3
(-2.5)
P 		520.4
(-3)
P 		1026
C 1360.9
(2)
P 		1527.1
(3)
P 		1268.3
(1.5)
P 		775.7
(-1.3)
P 		621.8
(-2.2)
P 		477.8
(-3)
P 		1049
avr 1204.3
(1.4) 		1469.9
(3) 		1247.8
(1.6) 		809.7
(-1) 		604.6
(-2.3) 		487.4
(-3) 		983
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 638.4
(3)
P 		242.8
(-1.2)
P 		357.7
(0.1)
P 		67
(-3)
P 		571
B 558.9
(3)
P 		280.2
(-1)
P 		326.6
(-0.3)
P 		135.9
(-3)
P 		423
C 576.5
(3)
P 		301.7
(-0.5)
P 		518.2
(2.3)
P 		100
(-3)
P 		477
avr 591.3
(3) 		274.9
(-0.9) 		400.8
(0.7) 		101
(-3) 		490

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 402
1 2 3 4 5 6 range
A 169.5
(-2.1)
P 		475.3
(3)
P 		280.4
(-0.2)
P 		215
(-1.3)
P 		178.3
(-1.9)
P 		113
(-3)
P 		362
B 463.6
(2)
P 		514.6
(3)
P 		288.4
(-1.2)
P 		213.5
(-2.6)
P 		244.3
(-2.1)
P 		194.1
(-3)
P 		321
C 504.4
(3)
P 		466
(2.3)
P 		322.8
(-0.1)
P 		156
(-3)
P 		201.2
(-2.2)
P 		160
(-2.9)
P 		348
avr 379.2
(1.1) 		485.3
(3) 		297.2
(-0.4) 		194.8
(-2.3) 		207.9
(-2) 		155.7
(-3) 		330
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175238_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20772
Sequence Type Exemplar sequence
Representative Public ID AU114285 NCBI
Target Description AU114285_rc /REP_DB=TREMBL Accession /GB=AU114285 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk717c7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062582 NCBI Caenorhabditis elegans intestinal acid PHOsphatase family member (pho-1) (pho-1) mRNA, complete cds. 11/11 A 
SNAP00000035698 ENSEMBL cdna:SNAP chromosome:CEL140:II:5233279:5235808:-1 11/11 None
GENEFINDER00000035700 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5233279:5235808:-1 11/11 None
EGAP2.3.1 ENSEMBL cdna:known chromosome:CEL140:II:5233134:5235822:-1 gene:EGAP2.3 11/11 None
EGAP2.3.2 ENSEMBL cdna:known chromosome:CEL140:II:5233134:5235902:-1 gene:EGAP2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5233304-5233709(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pho-1
Ensembl EGAP2.3
Entrez Gene 173896 Entrez gene
SwissProt Q19076 EMBL-EBI
WormBase CE04325 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3993 acid phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83593 Intestinal acid phosphatase protein 1 [Caenorhabditis elegans] ref|NP_494983.1| intestinal acid PHOsphatase family member (pho-1) [Caenorhabditis elegans] 0.0
blast CAE59070 Hypothetical protein CBG02356 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 3.9E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494983.1 1 426-448
EGAP2.3.1 1 426-448
EGAP2.3.2 1 426-448
SNAP00000035698 1 429-451
GENEFINDER00000035700 1 426-448
Sequence
Target Sequence 
>C. ELEGANS:175238_S_AT
gctgcagtccttttagagctttacattgatagtgtcgacaagaaaccatacttcaaaatg
gtatatcacgagcaagacggaagtggattcaaagatgtgactatgggaattcaaggatgt
cctcaaaatagttcatactgtgatttagatattctcagaaactttgccaatactatcaag
ccagatcaaccaatcgatcagtggtgcttaaccgatttgaacaagagttccagctttgct
aca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGCAGTCCTTTTAGAGCTTTACA 50 297 15 Antisense
GAGCTTTACATTGATAGTGTCGACA 67 389 30 Antisense
GTGTCGACAAGAAACCATACTTCAA 445 487 46 Antisense
GGTATATCACGAGCAAGACGGAAGT 629 505 74 Antisense
GGATGTCCTCAAAATAGTTCATACT 35 515 129 Antisense
GTTCATACTGTGATTTAGATATTCT 265 445 145 Antisense
ATTCTCAGAAACTTTGCCAATACTA 350 11 165 Antisense
GCCAATACTATCAAGCCAGATCAAC 362 277 180 Antisense
GCCAGATCAACCAATCGATCAGTGG 134 279 194 Antisense
GATCAGTGGTGCTTAACCGATTTGA 313 419 210 Antisense
GAACAAGAGTTCCAGCTTTGCTACA 116 353 233 Antisense
Sequence Source Affymetrix Proprietary Database