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[175222_at]

Description AV177156_rc /REP_DB=TREMBL Accession /GB=AV177156 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk525b3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 57      4grp_high_8_8 > 33      6grp_highest_nonorm > 50      6grp_total > 15      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 771
1 2 3 4 range
A 772.2
(3)
P 		615.8
(0.4)
P 		412.5
(-3)
P 		413
(-3)
P 		360
B 1106.6
(3)
P 		502.5
(-1.7)
P 		349.8
(-2.9)
P 		335.9
(-3)
P 		771
C 840.3
(3)
P 		474.7
(-1.6)
P 		376.2
(-2.8)
P 		362
(-3)
P 		478
avr 906.4
(3) 		531
(-1.2) 		379.5
(-2.9) 		370.3
(-3) 		536

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 580
1 2 3 4 5 6 range
A 754.3
(3)
P 		674.6
(2.2)
P 		311.2
(-1.7)
P 		263.7
(-2.2)
P 		297.2
(-1.8)
P 		184.4
(-3)
P 		570
B 642.3
(2.3)
P 		697
(3)
P 		357.2
(-1.4)
P 		343.4
(-1.6)
P 		233.5
(-3)
P 		299.2
(-2.1)
P 		464
C 763.9
(3)
P 		620.6
(1.4)
P 		407.9
(-0.9)
P 		216.8
(-3)
P 		232.7
(-2.8)
P 		239.5
(-2.8)
P 		547
avr 720.2
(3) 		664.1
(2.3) 		358.8
(-1.5) 		274.6
(-2.6) 		254.5
(-2.8) 		241
(-3) 		479
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 281.2
(3)
P 		133.7
(-1)
P 		107.8
(-1.7)
P 		60.5
(-3)
P 		221
B 352.9
(3)
P 		124.1
(-1.9)
P 		81.1
(-2.8)
P 		71.6
(-3)
P 		281
C 278.2
(3)
P 		136.5
(-0.9)
P 		131.1
(-1.1)
P 		61
(-3)
P 		217
avr 304.1
(3) 		131.4
(-1.3) 		106.7
(-1.9) 		64.4
(-3) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 240
1 2 3 4 5 6 range
A 139
(-0.1)
P 		239.9
(3)
P 		67.8
(-2.3)
P 		66.9
(-2.3)
P 		89.7
(-1.6)
P 		44.9
(-3)
P 		195
B 223.5
(2.6)
P 		232
(3)
P 		86.7
(-3)
P 		91
(-2.8)
P 		94.9
(-2.7)
P 		111.6
(-2)
P 		145
C 283.2
(3)
P 		189.4
(0.7)
P 		103.8
(-1.5)
P 		43.6
(-3)
P 		75.3
(-2.2)
P 		80.2
(-2.1)
P 		240
avr 215.2
(2.8) 		220.4
(3) 		86.1
(-2.3) 		67.2
(-3) 		86.6
(-2.2) 		78.9
(-2.5) 		153
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175222_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22251
Sequence Type Exemplar sequence
Representative Public ID AV177156 NCBI
Target Description AV177156_rc /REP_DB=TREMBL Accession /GB=AV177156 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk525b3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076903(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076903 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-14) (spp-14) mRNA, complete cds. 11/11 None
K09F5.3b ENSEMBL cdna:known chromosome:CEL140:X:7719422:7725508:1 gene:K09F5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181032 3 1 NM_076902 188058_at (11)
NM_076903 175222_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7725165-7725445(+) 100.0 100.0
Public Domain and Genome References
Ensembl K09F5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509304 SaPosin-like Protein family member (spp-14) [Caenorhabditis elegans] gb|AAL08045.2| Saposin-like protein family protein 14, isoform b [Caenorhabditis elegans] 0.0
blast NP_509303 SaPosin-like Protein family member (spp-14) [Caenorhabditis elegans] gb|AAB52676.1| Saposin-like protein family protein 14, isoform a [Caenorhabditis elegans] 0.0
blast CAE70262 Hypothetical protein CBG16766 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509304.2 7 12-34,54-76,219-238,248-270,302-324,380-402,409-431
K09F5.3b 7 12-34,54-76,219-238,248-270,302-324,380-402,409-431
Sequence
Target Sequence 
>C. ELEGANS:175222_AT
acggactgaattctactgtttatggaacaatttcctcgccactttctttgattttcgacc
cttgtaagtaagcgcgtaataattgcctcccgcatttttatgtataactgataatttctt
cttacgtttttctactcctcctcccttttttcatgtcaccccacaccaaaaatcatcaaa
aatcgtttcctttgttactgtactatattcccgttggatatcgggtacatttctttgaat
ttccacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGACTGAATTCTACTGTTTATGG 89 95 14 Antisense
TATGGAACAATTTCCTCGCCACTTT 473 655 34 Antisense
TTCTTTGATTTTCGACCCTTGTAAG 619 689 57 Antisense
TCGACCCTTGTAAGTAAGCGCGTAA 285 599 68 Antisense
AAGCGCGTAATAATTGCCTCCCGCA 61 149 83 Antisense
TTGCCTCCCGCATTTTTATGTATAA 72 703 96 Antisense
TGATAATTTCTTCTTACGTTTTTCT 28 565 122 Antisense
ATCGTTTCCTTTGTTACTGTACTAT 505 35 195 Antisense
GTTACTGTACTATATTCCCGTTGGA 394 445 207 Antisense
GTTGGATATCGGGTACATTTCTTTG 116 435 226 Antisense
GGTACATTTCTTTGAATTTCCACAA 167 505 237 Antisense
Sequence Source Affymetrix Proprietary Database