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[175220_s_at] protein kinase

Description AV176601_rc /REP_DB=TREMBL Accession /GB=AV176601 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk515c9 : 3prime end, single read.
Cluster 6grp_total > 76      4grp_total > 02      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 6.9
(-2.9)
A 		5.5
(-2.9)
A 		3.9
(-3)
A 		170.6
(3)
A 		167
B 5.3
(-3)
A 		8.4
(-2.9)
A 		6.4
(-3)
A 		202.1
(3)
A 		197
C 9.4
(-2.8)
A 		10.6
(-2.7)
A 		3.1
(-3)
A 		161.3
(3)
A 		158
avr 7.2
(-2.9) 		8.2
(-2.9) 		4.5
(-3) 		178
(3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 6.2
(-2)
A 		4.8
(-3)
A 		5
(-2.9)
A 		8.5
(-0.3)
A 		8.3
(-0.5)
A 		13.1
(3)
A 		8
B 6
(0.5)
A 		5.3
(-0.3)
A 		2.6
(-3)
A 		6.9
(1.4)
A 		5.1
(-0.5)
A 		8.5
(3)
A 		6
C 7
(-2.9)
A 		9.8
(-1.5)
A 		8.8
(-2)
A 		14.3
(0.7)
A 		6.7
(-3)
A 		18.9
(3)
A 		12
avr 6.4
(-2.3) 		6.6
(-2.2) 		5.5
(-3) 		9.9
(0.3) 		6.7
(-2.1) 		13.5
(3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 2.5
(-2.6)
A 		1.2
(-3)
A 		1
(-3)
A 		25
(3)
A 		24
B 1.7
(-3)
A 		2.1
(-2.9)
A 		1.5
(-3)
A 		43.1
(3)
A 		42
C 3.1
(-2.5)
A 		3
(-2.6)
A 		1.1
(-3)
A 		27.2
(3)
A 		26
avr 2.4
(-2.8) 		2.1
(-2.8) 		1.2
(-3) 		31.8
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 1.1
(-3)
A 		1.7
(-1.3)
A 		1.1
(-3)
A 		2.2
(0.1)
A 		2.5
(1)
A 		3.2
(3)
A 		2
B 2.1
(0.5)
A 		1.8
(-0.2)
A 		0.6
(-3)
A 		1.8
(-0.2)
A 		2.1
(0.5)
A 		3.2
(3)
A 		3
C 2.6
(-2.4)
A 		3
(-1.8)
A 		2.2
(-3)
A 		2.9
(-2)
A 		2.2
(-3)
A 		6.3
(3)
A 		4
avr 1.9
(-1.8) 		2.2
(-1.1) 		1.3
(-3) 		2.3
(-0.9) 		2.3
(-0.9) 		4.2
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175220_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21959
Sequence Type Exemplar sequence
Representative Public ID AV176601 NCBI
Target Description AV176601_rc /REP_DB=TREMBL Accession /GB=AV176601 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk515c9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070761(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070761 NCBI Caenorhabditis elegans R02C2.2 (R02C2.2) mRNA, complete cds. 11/11 None
SNAP00000019094 ENSEMBL cdna:SNAP chromosome:CEL140:V:244320:245675:-1 11/11 None
GENEFINDER00000019098 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:244320:245675:-1 11/11 None
R02C2.2 ENSEMBL cdna:known chromosome:CEL140:V:244298:245675:-1 gene:R02C2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:244307-244862(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl R02C2.2
Entrez Gene 178552 Entrez gene
WormBase CE39025 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48229 Hypothetical protein R02C2.2 [Caenorhabditis elegans] ref|NP_503162.2| R02C2.2 [Caenorhabditis elegans] 1.0E-169
blast AAC48229 Hypothetical protein R02C2.2 [Caenorhabditis elegans] ref|NP_503162.2| R02C2.2 [Caenorhabditis elegans] 1.0E-165
blast AAF59485 Checkpoint kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024233.1| CHeckpoint Kinase family member (chk-1) [Caenorhabditis elegans] sp|Q9N3Z3|CHK1_CAEEL Serine/threonine-protein kinase chk-1 8.0E-71
blast AAA93318 serine/threonine kinase 8.0E-71
blast AAF59485 Checkpoint kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024233.1| CHeckpoint Kinase family member (chk-1) [Caenorhabditis elegans] sp|Q9N3Z3|CHK1_CAEEL Serine/threonine-protein kinase chk-1 3.0E-69
blast AAA93318 serine/threonine kinase 3.0E-69
Protein Families
Method ID Description E-Value
hanks 1.7.2 AGC group; AGC VII Budding yeast DBF2/20; DBF20 5.0E-57
hanks 1.7.2 AGC group; AGC VII Budding yeast DBF2/20; DBF20 5.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.9E-61
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-64
Sequence
Target Sequence 
>C. ELEGANS:175220_S_AT
gacaactgccatgggagtttccgtgcatgacaaacaagcgctacttcttctggatgaaaa
accgggtcgcccatatcgacgcgtggaatgagctcagctgccgagccaagaaactgctct
ggaaaatgctttctccaggttcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAACTGCCATGGGAGTTTCCGTG 696 365 13 Antisense
GGGAGTTTCCGTGCATGACAAACAA 108 495 25 Antisense
GACAAACAAGCGCTACTTCTTCTGG 658 363 41 Antisense
CAAGCGCTACTTCTTCTGGATGAAA 161 187 47 Antisense
GATGAAAAACCGGGTCGCCCATATC 316 409 65 Antisense
TCGCCCATATCGACGCGTGGAATGA 243 599 79 Antisense
CGACGCGTGGAATGAGCTCAGCTGC 533 247 89 Antisense
TGGAATGAGCTCAGCTGCCGAGCCA 238 547 96 Antisense
GCTGCCGAGCCAAGAAACTGCTCTG 540 297 109 Antisense
AACTGCTCTGGAAAATGCTTTCTCC 468 141 124 Antisense
GGAAAATGCTTTCTCCAGGTTCAGA 340 527 133 Antisense
Sequence Source Affymetrix Proprietary Database