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[175203_s_at]

Description AU116437_rc /REP_DB=TREMBL Accession /GB=AU116437 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk744f6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 90      6grp_total > 78      4grp_total > 46      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 168.3
(3)
P 		98.5
(-3)
P 		102.7
(-2.6)
P 		135.3
(0.2)
P 		70
B 187.6
(3)
P 		99.9
(-2.9)
P 		121.2
(-1.5)
P 		98.3
(-3)
P 		89
C 151.4
(0.3)
P 		194.8
(3)
P 		121.5
(-1.6)
P 		99.3
(-3)
P 		96
avr 169.1
(3) 		131.1
(-0.9) 		115.1
(-2.6) 		111
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 150.5
(-0.5)
P 		118.7
(-1.6)
P 		159.9
(-0.2)
P 		74
(-3)
P 		147.9
(-0.6)
P 		260.4
(3)
P 		186
B 119.4
(-0.9)
P 		121.8
(-0.8)
P 		71.9
(-3)
P 		152.5
(0.6)
P 		146.9
(0.3)
P 		207.1
(3)
P 		135
C 173.7
(2.3)
P 		120.1
(-3)
P 		138.3
(-1.2)
P 		173.5
(2.3)
P 		181
(3)
P 		171
(2)
P 		61
avr 147.9
(-1.2) 		120.2
(-3) 		123.4
(-2.8) 		133.3
(-2.2) 		158.6
(-0.5) 		212.8
(3) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 61.3
(3)
P 		21.4
(-2.8)
P 		26.8
(-2)
P 		19.8
(-3)
P 		42
B 59.8
(3)
P 		24.7
(-2.4)
P 		28.1
(-1.9)
P 		21
(-3)
P 		39
C 50.1
(2.1)
P 		56
(3)
P 		42.4
(0.9)
P 		16.8
(-3)
P 		39
avr 57.1
(3) 		34
(-0.7) 		32.4
(-0.9) 		19.2
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 27.8
(-1.8)
P 		42.2
(0.1)
P 		34.8
(-0.8)
P 		18.8
(-3)
P 		44.6
(0.5)
P 		63.4
(3)
P 		45
B 41.6
(-0.6)
P 		40.5
(-0.7)
P 		17.5
(-3)
P 		40.4
(-0.7)
P 		59.7
(1.2)
P 		77.2
(3)
P 		60
C 64.4
(3)
P 		36.6
(-2.7)
P 		35.2
(-2.9)
P 		34.9
(-3)
P 		58.6
(1.8)
P 		57.3
(1.6)
P 		30
avr 44.6
(-0.5) 		39.8
(-1.3) 		29.2
(-3) 		31.4
(-2.6) 		54.3
(1.1) 		66
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175203_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21476
Sequence Type Exemplar sequence
Representative Public ID AU116437 NCBI
Target Description AU116437_rc /REP_DB=TREMBL Accession /GB=AU116437 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk744f6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069088
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000041295 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8682554:8686592:-1 None
F08B4.5.2 ENSEMBL cdna:known chromosome:CEL140:IV:8682471:8686593:-1 gene:F08B4.5 None
SNAP00000041286 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8682554:8687791:-1 None
NM_069088 NCBI Caenorhabditis elegans F08B4.5 (F08B4.5) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8682303-8682628(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08B4.5
Entrez Gene 177673 Entrez gene
SwissProt Q19196 EMBL-EBI
WormBase CE29749 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21914.1.S1_S_AT similar to DNA polymerase epsilon subunit 2 cfa
DROSOPHILA_2:1630620_AT dm
DROSGENOME1:154339_AT dm
CHICKEN:GGAAFFX.12131.1.S1_S_AT polymerase (DNA directed), epsilon 2 (p59 subunit) gga
HG-U133_PLUS_2:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U133A:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-FOCUS:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U133A_2:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HU35KSUBD:RC_AA448664_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
U133_X3P:G4505934_3P_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U95AV2:41085_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U95D:84336_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HU35KSUBD:RC_AA351146_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) hs
MOUSE430_2:1431440_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOE430B:1431440_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MG-U74AV2:101920_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOE430A:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOUSE430A_2:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOUSE430_2:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MU19KSUBA:TC19142_AT Polymerase (DNA directed), epsilon 2 (p59 subunit), mRNA (cDNA clone MGC:70176 IMAGE:30147626) mm
RG-U34C:RC_AI070935_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAE230B:1393982_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAT230_2:1393982_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAE230B:1396484_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAT230_2:1396484_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
YEAST_2:1770645_AT Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6260 DNA replication inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3887 DNA-directed DNA polymerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37729 Hypothetical protein F08B4.5 [Caenorhabditis elegans] ref|NP_501489.2| F08B4.5 [Caenorhabditis elegans] sp|Q19196|DPOE2_CAEEL Putative DNA polymerase epsilon subunit B (DNA polymerase II subunit B) 0.0
blast T29484 hypothetical protein F08B4.5 - Caenorhabditis elegans 0.0
blast CAE72481 Hypothetical protein CBG19657 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 1.0E-86
Pfam IPR003604 EMBL-EBI Zn-finger, U1-like 2.0E-28
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 1.3E-84
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 2.4E-81
Sequence
Target Sequence 
>C. ELEGANS:175203_S_AT
gatgattaagaattcatacccaattatatttccagctctaacatcaaaaatgtaatcata
ttcttcttccttttctgacatgcttcacaaatcaagcttacctattttaattatcaaatc
tccaattccattcccgtttttctttgatctgcaaaccatctcaatctcacaattttccat
tcgattttcatctaatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGATTAAGAATTCATACCCAATT 24 411 87 Antisense
CATACCCAATTATATTTCCAGCTCT 309 213 101 Antisense
AATTATATTTCCAGCTCTAACATCA 335 175 108 Antisense
TTCCTTTTCTGACATGCTTCACAAA 233 701 153 Antisense
CTTCACAAATCAAGCTTACCTATTT 83 221 169 Antisense
CAAGCTTACCTATTTTAATTATCAA 314 185 179 Antisense
TTATCAAATCTCCAATTCCATTCCC 370 679 197 Antisense
TTCCCGTTTTTCTTTGATCTGCAAA 283 699 217 Antisense
GATCTGCAAACCATCTCAATCTCAC 316 419 232 Antisense
ATTTTCCATTCGATTTTCATCTAAT 24 15 258 Antisense
TTCCATTCGATTTTCATCTAATTTT 64 701 261 Antisense
Sequence Source Affymetrix Proprietary Database