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[175159_at]

Description AV178698_rc /REP_DB=TREMBL Accession /GB=AV178698 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk570e8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 26      4grp_high_8_8 > 60      6grp_highest_nonorm > 84      6grp_total > 78      4grp_total > 60      6grp_highest_sub > Da      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 514.5
(0.8)
P 		510.7
(0.5)
P 		543.7
(3)
P 		463.6
(-3)
P 		80
B 516.5
(-0.2)
P 		565.2
(1.1)
P 		635
(3)
P 		412.9
(-3)
P 		222
C 543.4
(2.1)
P 		500.6
(0.7)
P 		573.3
(3)
P 		383.2
(-3)
P 		190
avr 524.8
(0.8) 		525.5
(0.9) 		584
(3) 		419.9
(-3) 		164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 496
1 2 3 4 5 6 range
A 577
(-2.1)
P 		579.9
(-2)
P 		607.1
(-1.5)
P 		680.9
(-0.2)
P 		524.7
(-3)
P 		860.5
(3)
P 		336
B 518.3
(-1.2)
P 		433
(-3)
P 		526
(-1)
P 		489.6
(-1.8)
P 		628.8
(1.2)
P 		710.6
(3)
P 		278
C 608.7
(0.6)
P 		424.9
(-2.1)
P 		573.8
(0.1)
P 		364.3
(-3)
P 		677.2
(1.6)
P 		768.3
(3)
P 		404
avr 568
(-1.2) 		479.3
(-3) 		569
(-1.2) 		511.6
(-2.4) 		610.2
(-0.4) 		779.8
(3) 		301
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 187.3
(3)
P 		110.9
(-0.8)
P 		142.1
(0.7)
P 		67.9
(-3)
P 		119
B 164.7
(3)
P 		139.6
(1)
P 		147.2
(1.6)
P 		88
(-3)
P 		77
C 179.9
(2.1)
P 		143.9
(0.5)
P 		199.8
(3)
P 		64.6
(-3)
P 		135
avr 177.3
(3) 		131.5
(0.4) 		163
(2.2) 		73.5
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 106.4
(-3)
P 		206.2
(2.8)
P 		132.2
(-1.5)
P 		172.7
(0.9)
P 		158.3
(0)
P 		209.5
(3)
P 		103
B 180.3
(-0.7)
P 		144.1
(-2.3)
P 		127.7
(-3)
P 		129.7
(-2.9)
P 		255.5
(2.6)
P 		265
(3)
P 		137
C 225.6
(2)
P 		129.7
(-1.2)
P 		146
(-0.6)
P 		73.3
(-3)
P 		219.1
(1.8)
P 		257.2
(3)
P 		184
avr 170.8
(-0.7) 		160
(-1.2) 		135.3
(-2.5) 		125.2
(-3) 		211
(1.3) 		243.9
(3) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175159_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21622
Sequence Type Exemplar sequence
Representative Public ID AV178698 NCBI
Target Description AV178698_rc /REP_DB=TREMBL Accession /GB=AV178698 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk570e8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028321(11), NM_001028322(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028321 NCBI Caenorhabditis elegans Y54G2A.2a (Y54G2A.2) mRNA, complete cds. 11/11 None
NM_001028322 NCBI Caenorhabditis elegans Y54G2A.2b (Y54G2A.2) mRNA, complete cds. 11/11 None
Y54G2A.2a ENSEMBL cdna:known chromosome:CEL140:IV:2788689:2796803:1 gene:Y54G2A.2 11/11 None
Y54G2A.2b ENSEMBL cdna:known chromosome:CEL140:IV:2788698:2796757:1 gene:Y54G2A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036474 8/11 Cross Hyb Matching Probes None
GENEFINDER00000036593 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2796456-2796736(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y54G2A.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68527 Hypothetical protein Y54G2A.2a [Caenorhabditis elegans] ref|NP_001023492.1| Y54G2A.2a [Caenorhabditis elegans] gb|AAG50213.1| putative guanylate-binding protein [Caenorhabditis elegans] 0.0
blast AAU05551 Hypothetical protein Y54G2A.2b [Caenorhabditis elegans] ref|NP_001023493.1| Y54G2A.2b [Caenorhabditis elegans] 0.0
blast EAA04985 ENSANGP00000017995 [Anopheles gambiae str. PEST] gb|EAA01072.3| ENSANGP00000019861 [Anopheles gambiae str. PEST] ref|XP_321004.2| ENSANGP00000019861 [Anopheles gambiae str. PEST] ref|XP_309266.2| ENSANGP00000017995 [Anopheles gambiae str. PEST] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR003191 EMBL-EBI Guanylate-binding protein 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023492.1 1 483-505
NP_001023493.1 1 483-505
Y54G2A.2a 1 483-505
Y54G2A.2b 1 483-505
Sequence
Target Sequence 
>C. ELEGANS:175159_AT
accaatttcatctcgccaaatgctaatcacttgggaccacttggcggtgcgattcaaatg
ggagaagctctcggagcaggaaatcgaactgcaaatgcgacggcggcccccggagccgct
gatggactcagaaaacggcattagatcaattattgctttttccactgaatttctcccctc
aaatgaccaatttcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCAATTTCATCTCGCCAAATGCTA 130 69 16 Antisense
CATCTCGCCAAATGCTAATCACTTG 4 213 24 Antisense
TAATCACTTGGGACCACTTGGCGGT 672 629 39 Antisense
ACCACTTGGCGGTGCGATTCAAATG 604 83 51 Antisense
CAAATGGGAGAAGCTCTCGGAGCAG 385 191 70 Antisense
AGCTCTCGGAGCAGGAAATCGAACT 478 77 81 Antisense
TCGAACTGCAAATGCGACGGCGGCC 62 601 99 Antisense
GGAGCCGCTGATGGACTCAGAAAAC 111 523 127 Antisense
GACTCAGAAAACGGCATTAGATCAA 377 371 140 Antisense
TTGCTTTTTCCACTGAATTTCTCCC 423 703 168 Antisense
CTCCCCTCAAATGACCAATTTCTTC 541 231 188 Antisense
Sequence Source Affymetrix Proprietary Database