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[175147_at]

Description AV179717_rc /REP_DB=TREMBL Accession /GB=AV179717 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk597b1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 89      4grp_high_8_8 > 30      6grp_highest_nonorm > 94      6grp_total > 98      4grp_total > 33      6grp_highest_sub > Cu      6grp_cgc > 79      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 77.1
(-3)
A 		265.2
(3)
P 		121.8
(-1.6)
P 		216.4
(1.4)
P 		188
B 112.3
(-3)
M 		162.3
(0.4)
P 		124
(-2.2)
P 		201.1
(3)
P 		89
C 94
(-3)
P 		205.8
(0.3)
P 		297.5
(3)
P 		232.3
(1.1)
P 		204
avr 94.5
(-3) 		211.1
(2.7) 		181.1
(1.3) 		216.6
(3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 992
1 2 3 4 5 6 range
A 252.1
(-1.9)
P 		139.4
(-3)
P 		227.3
(-2.2)
P 		342.6
(-1.1)
P 		488.2
(0.3)
P 		765.6
(3)
P 		626
B 380.3
(-1.7)
P 		242.8
(-2.8)
P 		220.1
(-3)
P 		549.1
(-0.3)
P 		942.5
(3)
P 		914.1
(2.8)
P 		722
C 578.7
(-0.4)
P 		263.1
(-2.3)
P 		156.2
(-3)
P 		330.4
(-1.9)
P 		1131
(3)
P 		887.6
(1.5)
P 		975
avr 403.7
(-1.1) 		215.1
(-2.9) 		201.2
(-3) 		407.4
(-1.1) 		853.9
(3) 		855.8
(3) 		655
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 28.1
(-3)
A 		57.6
(3)
P 		31.9
(-2.2)
P 		31.7
(-2.3)
P 		30
B 35.8
(0)
M 		40.1
(1.9)
P 		28.7
(-3)
P 		42.8
(3)
P 		14
C 31.1
(-3)
P 		59.2
(-0.7)
P 		103.7
(3)
P 		39.2
(-2.3)
P 		73
avr 31.7
(-3) 		52.3
(2.4) 		54.8
(3) 		37.9
(-1.4) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 46.5
(-3)
P 		49.6
(-2.9)
P 		49.5
(-2.9)
P 		86.9
(-1.3)
P 		147.3
(1.3)
P 		186.4
(3)
P 		140
B 132.3
(-1.6)
P 		80.8
(-2.5)
P 		53.4
(-3)
P 		145.4
(-1.3)
P 		382.9
(3)
P 		340.9
(2.2)
P 		330
C 214.5
(0.2)
P 		80.3
(-2.3)
P 		39.8
(-3)
P 		66.4
(-2.5)
P 		366
(3)
P 		297.1
(1.7)
P 		326
avr 131.1
(-1) 		70.2
(-2.5) 		47.6
(-3) 		99.6
(-1.8) 		298.7
(3) 		274.8
(2.4) 		251
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175147_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20791
Sequence Type Exemplar sequence
Representative Public ID AV179717 NCBI
Target Description AV179717_rc /REP_DB=TREMBL Accession /GB=AV179717 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk597b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062634(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062634 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-5) (dhs-5) mRNA, complete cds. 11/11 None
F56D1.5 ENSEMBL cdna:known chromosome:CEL140:II:5453817:5455933:1 gene:F56D1.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013097 5/11 Cross Hyb Matching Probes None
GENEFINDER00000013106 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5455579-5455872(+) 87.03 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dhs-5
Ensembl F56D1.5
Entrez Gene 173930 Entrez gene
SwissProt Q10130 EMBL-EBI
WormBase CE28036 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68388 Dehydrogenases, short chain protein 5 [Caenorhabditis elegans] ref|NP_495035.1| DeHydrogenases, Short chain family member (dhs-5) [Caenorhabditis elegans] sp|Q10130|DHS5_CAEEL Putative oxidoreductase dhs-5 0.0
blast CAE59108 Hypothetical protein CBG02403 [Caenorhabditis briggsae] 1.0E-167
blast T30110 hypothetical protein F56D1.5 - Caenorhabditis elegans 1.0E-147
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 5.5E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495035.1 2 38-55,62-79
F56D1.5 2 38-55,62-79
Sequence
Target Sequence 
>C. ELEGANS:175147_AT
gtgacagaattgcacaatacgagcaataacccagaacaggaaatttcacttcaagaattg
aatgaggaaacggtggtgcctacaacaaagaaaactgtcactcgtgactcggccactgcc
taatactgaaaagaatagttgtttttaattttcattttccctctgaaattgtgctttaca
tacaaattttaatttctaataacatgttcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACAGAATTGCACAATACGAGCA 378 481 64 Antisense
AATACGAGCAATAACCCAGAACAGG 261 173 79 Antisense
GAACAGGAAATTTCACTTCAAGAAT 687 351 97 Antisense
GAATGAGGAAACGGTGGTGCCTACA 627 331 123 Antisense
AAAACTGTCACTCGTGACTCGGCCA 276 131 154 Antisense
ACTCGGCCACTGCCTAATACTGAAA 678 99 170 Antisense
GGCCACTGCCTAATACTGAAAAGAA 24 543 174 Antisense
TTCATTTTCCCTCTGAAATTGTGCT 232 687 214 Antisense
TTCCCTCTGAAATTGTGCTTTACAT 542 697 220 Antisense
TGTGCTTTACATACAAATTTTAATT 219 555 233 Antisense
TTTAATTTCTAATAACATGTTCTTG 371 673 251 Antisense
Sequence Source Affymetrix Proprietary Database