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[175136_at] NADH-ubiquinone oxidoreductase

Description AV202808 /REP_DB=TREMBL Accession /GB=AV202808 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk555b11 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 37      4grp_high_8_8 > 01      6grp_highest_nonorm > 57      6grp_total > 52      4grp_total > 07      6grp_highest_sub > 03      6grp_cgc > 91      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1134
1 2 3 4 range
A 2332.1
(3)
P 		1671.9
(-3)
P 		2316.9
(2.9)
P 		2304.6
(2.8)
P 		660
B 2415.5
(-2.5)
P 		2471.8
(-1.7)
P 		2379.1
(-3)
P 		2806.2
(3)
P 		427
C 2625.4
(1.7)
P 		2456.1
(-3)
P 		2488.3
(-2.1)
P 		2671.2
(3)
P 		215
avr 2457.7
(0.9) 		2199.9
(-3) 		2394.8
(-0) 		2594
(3) 		394

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1309
1 2 3 4 5 6 range
A 2201.6
(-2.1)
P 		2649.3
(0)
P 		3290.8
(3)
P 		2806.6
(0.7)
P 		2236.3
(-1.9)
P 		2005
(-3)
P 		1286
B 2506.3
(-0.9)
P 		2254.4
(-2.1)
P 		3314.1
(3)
P 		2639.7
(-0.3)
P 		2329.3
(-1.8)
P 		2074.9
(-3)
P 		1239
C 2716
(2.8)
P 		2252.4
(-2.1)
P 		2702.3
(2.6)
P 		2682.8
(2.4)
P 		2739.9
(3)
P 		2163.9
(-3)
P 		576
avr 2474.6
(-0.7) 		2385.4
(-1.2) 		3102.4
(3) 		2709.7
(0.7) 		2435.2
(-0.9) 		2081.3
(-3) 		1021
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 532
1 2 3 4 range
A 849.1
(3)
P 		362.9
(-2.7)
P 		605.7
(0.1)
P 		337.3
(-3)
P 		512
B 770.3
(3)
P 		610.5
(-1.4)
P 		551.3
(-3)
P 		598
(-1.7)
P 		219
C 869.1
(3)
P 		706.2
(0.7)
P 		867.3
(3)
P 		450.4
(-3)
P 		419
avr 829.5
(3) 		559.9
(-1.4) 		674.8
(0.5) 		461.9
(-3) 		368

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 601
1 2 3 4 5 6 range
A 405.9
(-3)
P 		942.2
(3)
P 		716.7
(0.5)
P 		711.8
(0.4)
P 		674.8
(0)
P 		488.2
(-2.1)
P 		536
B 872.1
(1.2)
P 		750.4
(-1.8)
P 		804.8
(-0.4)
P 		699.2
(-3)
P 		946.4
(3)
P 		773.7
(-1.2)
P 		247
C 1006.7
(3)
P 		687.3
(-1.1)
P 		687.8
(-1.1)
P 		539.5
(-3)
P 		886.6
(1.5)
P 		724.4
(-0.6)
P 		467
avr 761.6
(0.6) 		793.3
(1.6) 		736.4
(-0.2) 		650.2
(-3) 		835.9
(3) 		662.1
(-2.6) 		186
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175136_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21471
Sequence Type Exemplar sequence
Representative Public ID AV202808 NCBI
Target Description AV202808 /REP_DB=TREMBL Accession /GB=AV202808 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk555b11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070389(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070389 NCBI Caenorhabditis elegans Y57G11C.12 (Y57G11C.12) mRNA, complete cds. 11/11 None
SNAP00000003480 ENSEMBL cdna:SNAP chromosome:CEL140:IV:14796926:14797412:-1 10/11 None
Y57G11C.12.2 ENSEMBL cdna:known chromosome:CEL140:IV:14796393:14797399:-1 gene:Y57G11C.12 11/11 A 
Y57G11C.12.1 ENSEMBL cdna:known chromosome:CEL140:IV:14796393:14797435:-1 gene:Y57G11C.12 11/11 A 
Y57G11C.12.4 ENSEMBL cdna:known chromosome:CEL140:IV:14796393:14797382:-1 gene:Y57G11C.12 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Y57G11C.12.3 8/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14796812-14797152(-) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase
Gene Symbol nuo-3
Ensembl Y57G11C.12
Entrez Gene 178404 Entrez gene
SwissProt O18236 EMBL-EBI
WormBase CE14948 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5743 mitochondrial inner membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16651 oxidoreductase activity, acting on NADH or NADPH inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16513 Hypothetical protein Y57G11C.12 [Caenorhabditis elegans] ref|NP_502790.1| Y57G11C.12 [Caenorhabditis elegans] 1.0E-71
blast CAE73906 Hypothetical protein CBG21512 [Caenorhabditis briggsae] 1.0E-65
blast AAY66861 unknown [Ixodes scapularis] 2.0E-19
blast AAF28978 HSPC300 [Homo sapiens] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR008011 EMBL-EBI Complex 1 LYR protein 1.8E-24
Sequence
Target Sequence 
>C. ELEGANS:175136_AT
gattattgccgataacatcaagaacatcgctaacttcttgtccagctttgagctctcttg
ccgcacaaaactagcgactctcagcgacaagcttaaccttttggaacgaaaggtcgaatt
tcttgaagcccgtgtcaccaaaggagatactctttcgtaataatgtctgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTATTGCCGATAACATCAAGAAC 442 429 14 Antisense
GAACATCGCTAACTTCTTGTCCAGC 606 349 35 Antisense
AACTTCTTGTCCAGCTTTGAGCTCT 361 139 45 Antisense
GCTCTCTTGCCGCACAAAACTAGCG 247 301 65 Antisense
AAAACTAGCGACTCTCAGCGACAAG 190 131 80 Antisense
GCGACTCTCAGCGACAAGCTTAACC 643 289 87 Antisense
GCGACAAGCTTAACCTTTTGGAACG 430 287 97 Antisense
AAGGTCGAATTTCTTGAAGCCCGTG 594 141 123 Antisense
TCTTGAAGCCCGTGTCACCAAAGGA 655 587 134 Antisense
TCACCAAAGGAGATACTCTTTCGTA 284 611 148 Antisense
TACTCTTTCGTAATAATGTCTGCAA 693 643 161 Antisense
Sequence Source Affymetrix Proprietary Database