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[175133_at]

Description AV203289 /REP_DB=TREMBL Accession /GB=AV203289 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk574e11 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 43      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 91      6grp_total > 10      4grp_total > 40      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 177.3
(-1)
P 		168.7
(-3)
P 		191.3
(2.2)
P 		194.6
(3)
P 		26
B 128.8
(-3)
P 		167.3
(-1.3)
P 		268.4
(3)
P 		147.2
(-2.2)
P 		140
C 173.7
(-3)
P 		174.4
(-3)
P 		299.7
(3)
P 		195.4
(-2)
P 		126
avr 159.9
(-3) 		170.1
(-2.3) 		253.1
(3) 		179.1
(-1.8) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 247
1 2 3 4 5 6 range
A 74.7
(-3)
P 		157.2
(-0.5)
P 		274.2
(3)
P 		165.9
(-0.3)
P 		198.2
(0.7)
P 		148.1
(-0.8)
P 		200
B 197.1
(-2)
P 		321.2
(3)
P 		283.1
(1.5)
P 		172.5
(-3)
P 		253.6
(0.3)
P 		218.5
(-1.1)
P 		149
C 199.9
(0.6)
P 		247.7
(3)
P 		153.6
(-1.7)
P 		127.8
(-3)
P 		232.8
(2.3)
P 		222.2
(1.7)
P 		120
avr 157.2
(-2.9) 		242
(3) 		237
(2.7) 		155.4
(-3) 		228.2
(2) 		196.3
(-0.2) 		87
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 64.6
(3)
P 		36.6
(-1.7)
P 		50
(0.6)
P 		28.5
(-3)
P 		36
B 41.1
(-1.1)
P 		41.3
(-1.1)
P 		62.2
(3)
P 		31.4
(-3)
P 		31
C 57.5
(-0.9)
P 		50.1
(-1.6)
P 		104.4
(3)
P 		32.9
(-3)
P 		72
avr 54.4
(0.4) 		42.7
(-1.3) 		72.2
(3) 		30.9
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 13.8
(-3)
P 		55.9
(2.5)
P 		59.7
(3)
P 		42.1
(0.7)
P 		59.8
(3)
P 		36.1
(-0.1)
P 		46
B 68.6
(-0.8)
P 		106.9
(3)
P 		68.8
(-0.7)
P 		45.7
(-3)
P 		103
(2.6)
P 		81.5
(0.5)
P 		61
C 74.1
(2.8)
P 		75.6
(3)
P 		39.1
(-1.4)
P 		25.7
(-3)
P 		75.3
(3)
P 		74.4
(2.9)
P 		50
avr 52.2
(-0.9) 		79.5
(3) 		55.9
(-0.4) 		37.8
(-3) 		79.4
(3) 		64
(0.8) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175133_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20932
Sequence Type Exemplar sequence
Representative Public ID AV203289 NCBI
Target Description AV203289 /REP_DB=TREMBL Accession /GB=AV203289 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk574e11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065652(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065652 NCBI Caenorhabditis elegans C27F2.9 (C27F2.9) mRNA, complete cds. 11/11 None
GENEFINDER00000001026 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4961914:4963903:-1 9/11 None
C27F2.9 ENSEMBL cdna:known chromosome:CEL140:III:4961839:4963031:-1 gene:C27F2.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065651 4/11 Cross Hyb Matching Probes None
SNAP00000001013 4/11 Cross Hyb Matching Probes None
C27F2.5.2 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4961848-4962181(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27F2.9
Entrez Gene 175673 Entrez gene
SwissProt Q95QU1 EMBL-EBI
WormBase CE37750 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.30007.1.S1_AT similar to RIKEN cDNA 2610003J06 cfa
CHICKEN:GGA.7133.1.S1_AT similar to RIKEN cDNA 2610003J06 gga
HU35KSUBA:AA089853_AT hypothetical LOC339123 hs
HU35KSUBD:RC_AA432370_AT hypothetical LOC339123 hs
U133_X3P:HS.25197.2.A1_3P_AT hypothetical LOC339123 hs
HG-U133_PLUS_2:224886_AT hypothetical LOC339123 hs
HG-U133B:224886_AT hypothetical LOC339123 hs
HG-U95C:65828_AT hypothetical LOC339123 hs
MOE430A:1424743_AT RIKEN cDNA 2610003J06 gene mm
MOUSE430A_2:1424743_AT RIKEN cDNA 2610003J06 gene mm
MOUSE430_2:1424743_AT RIKEN cDNA 2610003J06 gene mm
MG-U74AV2:94520_AT RIKEN cDNA 2610003J06 gene (2610003J06Rik), mRNA mm
MU11KSUBA:AA003161_S_AT RIKEN cDNA 2610003J06 gene (2610003J06Rik), mRNA mm
MU19KSUBC:TC39463_AT RIKEN cDNA 2610003J06 gene (2610003J06Rik), mRNA mm
RG-U34B:RC_AA851397_AT similar to RIKEN cDNA 2610003J06 (predicted) rn
RG-U34C:RC_AA818462_F_AT similar to RIKEN cDNA 2610003J06 (predicted) rn
RAE230A:1374124_AT similar to RIKEN cDNA 2610003J06 (predicted) rn
RAT230_2:1374124_AT similar to RIKEN cDNA 2610003J06 (predicted) rn
RAE230A:1371903_AT STIP1 homology and U-Box containing protein 1 (predicted) rn
RAT230_2:1371903_AT STIP1 homology and U-Box containing protein 1 (predicted) rn
RG-U34A:RC_AA892120_AT STIP1 homology and U-Box containing protein 1 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast NP_498053 C27F2.9 [Caenorhabditis elegans] gb|AAK67216.3| Hypothetical protein C27F2.9 [Caenorhabditis elegans] 1.0E-148
blast NP_498053 C27F2.9 [Caenorhabditis elegans] gb|AAK67216.3| Hypothetical protein C27F2.9 [Caenorhabditis elegans] 1.0E-145
blast CAE71313 Hypothetical protein CBG18204 [Caenorhabditis briggsae] 3.0E-81
blast XP_547212 PREDICTED: similar to RIKEN cDNA 2610003J06 [Canis familiaris] 3.0E-67
blast XP_547212 PREDICTED: similar to RIKEN cDNA 2610003J06 [Canis familiaris] 1.0E-66
Sequence
Target Sequence 
>C. ELEGANS:175133_AT
ggaatggtacacatatgactatccaaaattgtcccgcgacgaacttccattggaatgcga
aatgaaatctggagaagtaatctattttcccgataaatggtggcatgccactttaaacat
tgaatcttctgtttttattagcacttttttaagtccatagaccttattattgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAATGGTACACATATGACTATCCA 265 533 67 Antisense
GACTATCCAAAATTGTCCCGCGACG 17 373 83 Antisense
TCCCGCGACGAACTTCCATTGGAAT 302 597 98 Antisense
GGAATGCGAAATGAAATCTGGAGAA 335 531 118 Antisense
GGAGAAGTAATCTATTTTCCCGATA 97 515 137 Antisense
TTTTCCCGATAAATGGTGGCATGCC 587 675 151 Antisense
GGTGGCATGCCACTTTAAACATTGA 388 499 165 Antisense
GCCACTTTAAACATTGAATCTTCTG 188 279 173 Antisense
GAATCTTCTGTTTTTATTAGCACTT 52 329 188 Antisense
AGCACTTTTTTAAGTCCATAGACCT 403 75 206 Antisense
TAAGTCCATAGACCTTATTATTGTT 341 637 216 Antisense
Sequence Source Affymetrix Proprietary Database