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[175096_at]

Description AV194500 /REP_DB=TREMBL Accession /GB=AV194500 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk630b7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 71      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 54      6grp_total > 71      4grp_total > 22      6grp_highest_sub > 03_04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 661
1 2 3 4 range
A 908.3
(3)
P 		561.2
(-1.7)
P 		460.5
(-3)
P 		816.3
(1.8)
P 		448
B 738.9
(0.1)
P 		545
(-1.7)
P 		399.6
(-3)
P 		1061
(3)
P 		661
C 986.4
(3)
P 		466
(-3)
P 		500.2
(-2.6)
P 		867
(1.6)
P 		520
avr 877.9
(2.5) 		524.1
(-2.1) 		453.4
(-3) 		914.8
(3) 		461

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1727
1 2 3 4 5 6 range
A 605.7
(-2.8)
P 		1016.2
(-1.4)
P 		2286.6
(3)
P 		2151.9
(2.5)
P 		1106.4
(-1.1)
P 		560.1
(-3)
P 		1727
B 918.9
(-1.9)
P 		1288.5
(-0.2)
P 		2002.7
(3)
P 		1926.9
(2.7)
P 		1427.2
(0.4)
P 		668
(-3)
P 		1335
C 892.9
(-2.1)
P 		1236.3
(-0.4)
P 		1253.2
(-0.4)
P 		1757.3
(2.1)
P 		1932.6
(3)
P 		716
(-3)
P 		1217
avr 805.8
(-2.3) 		1180.3
(-0.5) 		1847.5
(2.5) 		1945.4
(3) 		1488.7
(0.9) 		648
(-3) 		1297
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 238
1 2 3 4 range
A 330.7
(3)
P 		121.8
(-2.9)
P 		120.4
(-3)
P 		119.5
(-3)
P 		211
B 235.6
(3)
P 		134.6
(-1.2)
P 		92.6
(-3)
P 		226.1
(2.6)
P 		143
C 326.5
(3)
P 		134
(-3)
P 		174.3
(-1.7)
P 		146.2
(-2.6)
P 		193
avr 297.6
(3) 		130.1
(-3) 		129.1
(-3) 		163.9
(-1.8) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 514
1 2 3 4 5 6 range
A 111.7
(-3)
P 		361.4
(0.5)
P 		498
(2.3)
P 		545.8
(3)
P 		333.9
(0.1)
P 		136.4
(-2.7)
P 		434
B 319.7
(-1.7)
P 		428.9
(0.3)
P 		486.3
(1.3)
P 		510.4
(1.7)
P 		579.8
(3)
P 		249.1
(-3)
P 		331
C 331
(-1.6)
P 		377.2
(-0.9)
P 		319
(-1.8)
P 		353.4
(-1.2)
P 		625.3
(3)
P 		239.7
(-3)
P 		386
avr 254.1
(-2.1) 		389.2
(0.6) 		434.4
(1.5) 		469.9
(2.2) 		513
(3) 		208.4
(-3) 		305
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175096_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20378
Sequence Type Exemplar sequence
Representative Public ID AV194500 NCBI
Target Description AV194500 /REP_DB=TREMBL Accession /GB=AV194500 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk630b7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059402(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059402 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-15) (spp-15) mRNA, complete cds. 11/11 None
C48E7.10 ENSEMBL cdna:known chromosome:CEL140:I:6257712:6258202:-1 gene:C48E7.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002340 8/11 Cross Hyb Matching Probes None
GENEFINDER00000002349 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6257691-6258082(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-15
Ensembl C48E7.10
Entrez Gene 172318 Entrez gene
SwissProt Q9BIC4 EMBL-EBI
WormBase CE26915 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21363 Saposin-like protein family protein 15 [Caenorhabditis elegans] ref|NP_491803.1| SaPosin-like Protein family member (spp-15) [Caenorhabditis elegans] 2.0E-56
blast CAE67147 Hypothetical protein CBG12572 [Caenorhabditis briggsae] 9.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 6.3E-7
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 6.1E-10
Sequence
Target Sequence 
>C. ELEGANS:175096_AT
ttggcaataactttgactgtttcaaacgtggaaggcgccaagaaaatgcattcggatacg
tcaaaaccactctgcggactttgtgttaatgttgtaaagcagctcgatcaggttttggaa
cacggaggtgatattgaagcggcagttgacaaattttgcaaagaagatgttccatcattt
atggttgatatgtgtgaaaaggtgatcgagaagaatttggaatacattatcaataaactg
aaggatcacgaagaagccgataagatttgcactgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGCAATAACTTTGACTGTTTCAA 585 705 16 Antisense
GACTGTTTCAAACGTGGAAGGCGCC 663 369 30 Antisense
AAGGCGCCAAGAAAATGCATTCGGA 657 161 47 Antisense
GCATTCGGATACGTCAAAACCACTC 646 305 63 Antisense
AAAACCACTCTGCGGACTTTGTGTT 245 129 78 Antisense
CTCTGCGGACTTTGTGTTAATGTTG 136 231 85 Antisense
GTTAATGTTGTAAAGCAGCTCGATC 709 445 100 Antisense
GCAGCTCGATCAGGTTTTGGAACAC 300 313 114 Antisense
GAGGTGATATTGAAGCGGCAGTTGA 578 401 140 Antisense
GAAGCGGCAGTTGACAAATTTTGCA 705 341 151 Antisense
GAAGCCGATAAGATTTGCACTGATA 404 343 268 Antisense
Sequence Source Affymetrix Proprietary Database