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[175089_at]

Description AV201688 /REP_DB=TREMBL Accession /GB=AV201688 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk540b8 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 69      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 83      6grp_total > 79      4grp_total > 02      6grp_highest_sub > Cu      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2345
1 2 3 4 range
A 627.8
(-2.7)
P 		556.2
(-3)
P 		595
(-2.8)
P 		2022.5
(3)
P 		1466
B 503.3
(-2.9)
P 		619.5
(-2.6)
P 		478
(-3)
P 		2587.2
(3)
P 		2109
C 540.8
(-3)
P 		522.7
(-3)
P 		659
(-2.6)
P 		2823.2
(3)
P 		2301
avr 557.3
(-3) 		566.1
(-3) 		577.3
(-2.9) 		2477.6
(3) 		1920

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 370
1 2 3 4 5 6 range
A 439.2
(-3)
P 		505.3
(-0.4)
P 		469.2
(-1.8)
P 		499.2
(-0.6)
P 		569
(2.2)
P 		589.3
(3)
P 		150
B 542.3
(1.3)
P 		474.6
(-0)
P 		317.4
(-3)
P 		405.5
(-1.3)
P 		534.6
(1.1)
P 		633
(3)
P 		316
C 524
(-2.1)
P 		513.1
(-2.4)
P 		495.4
(-3)
P 		570
(-0.7)
P 		538.7
(-1.6)
P 		687.3
(3)
P 		192
avr 501.8
(-0.9) 		497.7
(-1) 		427.3
(-3) 		491.6
(-1.2) 		547.4
(0.4) 		636.5
(3) 		209
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 441
1 2 3 4 range
A 228.6
(0.7)
P 		120.7
(-3)
P 		155.6
(-1.8)
P 		296.1
(3)
P 		175
B 160.5
(-2.3)
P 		153
(-2.4)
P 		110.8
(-3)
P 		551.3
(3)
P 		441
C 179
(-2.5)
P 		150.3
(-3)
P 		229.7
(-1.5)
P 		476
(3)
P 		326
avr 189.4
(-2) 		141.3
(-3) 		165.4
(-2.5) 		441.1
(3) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 81
(-3)
P 		179.7
(3)
P 		102.2
(-1.7)
P 		126.6
(-0.2)
P 		171.7
(2.5)
P 		143.5
(0.8)
P 		99
B 188.7
(1.2)
P 		158
(0.1)
P 		77.1
(-3)
P 		107.4
(-1.9)
P 		217.2
(2.3)
P 		236
(3)
P 		159
C 194.2
(1.1)
P 		156.6
(-0.8)
P 		126.1
(-2.4)
P 		114.6
(-3)
P 		174.3
(0.1)
P 		230.1
(3)
P 		116
avr 154.6
(0.1) 		164.8
(0.7) 		101.8
(-3) 		116.2
(-2.1) 		187.7
(2.1) 		203.2
(3) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175089_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21170
Sequence Type Exemplar sequence
Representative Public ID AV201688 NCBI
Target Description AV201688 /REP_DB=TREMBL Accession /GB=AV201688 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk540b8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065224(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065224 NCBI Caenorhabditis elegans Y71H2AM.1 (Y71H2AM.1) mRNA, complete cds. 11/11 None
Y71H2AM.1.1 ENSEMBL cdna:known chromosome:CEL140:III:2827060:2833780:1 gene:Y71H2AM.1 11/11 None
Y71H2AM.1.2 ENSEMBL cdna:known chromosome:CEL140:III:2827060:2833779:1 gene:Y71H2AM.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000014460 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2833085-2833738(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.1
Entrez Gene 175399 Entrez gene
SwissProt Q9BL28 EMBL-EBI
WormBase CE37451 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:20507_AT glycoprotease M22 family protein at
ATH1-121501:254273_AT glycoprotease M22 family protein at
DROSOPHILA_2:1641223_AT dm
DROSGENOME1:148887_AT dm
HU35KSUBB:RC_T17353_S_AT O-sialoglycoprotein endopeptidase hs
HU35KSUBD:RC_AA401938_AT O-sialoglycoprotein endopeptidase hs
HU35KSUBD:RC_H99870_AT O-sialoglycoprotein endopeptidase hs
HG-U133A:209450_AT O-sialoglycoprotein endopeptidase hs
HG-U133_PLUS_2:209450_AT O-sialoglycoprotein endopeptidase hs
HG-U133A_2:209450_AT O-sialoglycoprotein endopeptidase hs
U133_X3P:G13358863_3P_AT O-sialoglycoprotein endopeptidase hs
HG-U95AV2:38723_AT O-sialoglycoprotein endopeptidase hs
HG-U133_PLUS_2:242930_AT O-sialoglycoprotein endopeptidase hs
HG-U133B:242930_AT O-sialoglycoprotein endopeptidase hs
HG-U95E:78863_AT O-sialoglycoprotein endopeptidase hs
HU35KSUBB:RC_AA017219_AT O-sialoglycoprotein endopeptidase hs
MOE430A:1418529_AT O-sialoglycoprotein endopeptidase mm
MOUSE430A_2:1418529_AT O-sialoglycoprotein endopeptidase mm
MOUSE430_2:1418529_AT O-sialoglycoprotein endopeptidase mm
MG-U74BV2:109751_AT O-sialoglycoprotein endopeptidase mm
MG-U74CV2:168285_I_AT O-sialoglycoprotein endopeptidase mm
MG-U74CV2:170988_R_AT O-sialoglycoprotein endopeptidase mm
RAE230A:1389117_AT O-sialoglycoprotein endopeptidase (predicted) rn
RAT230_2:1389117_AT O-sialoglycoprotein endopeptidase (predicted) rn
YEAST_2:1769600_AT Putative glycoprotease that interacts with Bud32p, which is a member of the novel protein kinase piD261 family Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8450 O-sialoglycoprotein endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29978 Hypothetical protein Y71H2AM.1 [Caenorhabditis elegans] ref|NP_497625.3| Y71H2AM.1 [Caenorhabditis elegans] 0.0
blast CAE57513 Hypothetical protein CBG00488 [Caenorhabditis briggsae] 1.0E-133
blast AAH54300 Osgep-prov protein [Xenopus laevis] sp|Q7SYR1|GCP_XENLA Probable O-sialoglycoprotein endopeptidase 1.0E-133
Protein Domains
Database ID Description E-Value
Pfam IPR000905 EMBL-EBI Peptidase M22, glycoprotease 2.7E-99
Sequence
Target Sequence 
>C. ELEGANS:175089_AT
tcctgttcttcacacgttttttgcacaaaaaggaacgggaaacaaaatggtcgacggatg
tgctgagaaattcgagcaaatgagttcatggattgtgctgatttcaattgttccaataac
attcagctttataactgtcattcttttgtgctcatctcttctaaaagtgaagaaaatgcg
tggaatttgtgatggaatccattcgaatgctcattggttgtctagaactgctgttcgtgt
tggaattgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTGTTCTTCACACGTTTTTTGCA 312 593 20 Antisense
GTTCTTCACACGTTTTTTGCACAAA 664 443 24 Antisense
AATGGTCGACGGATGTGCTGAGAAA 38 163 65 Antisense
GGATTGTGCTGATTTCAATTGTTCC 278 509 109 Antisense
CAATTGTTCCAATAACATTCAGCTT 623 179 124 Antisense
AACATTCAGCTTTATAACTGTCATT 92 139 137 Antisense
TAACTGTCATTCTTTTGTGCTCATC 407 639 151 Antisense
TTTGTGCTCATCTCTTCTAAAAGTG 260 661 164 Antisense
GGAATCCATTCGAATGCTCATTGGT 585 531 213 Antisense
GCTCATTGGTTGTCTAGAACTGCTG 187 301 228 Antisense
GAACTGCTGTTCGTGTTGGAATTGG 228 349 244 Antisense
Sequence Source Affymetrix Proprietary Database