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[175073_s_at] Elongation factor Tu family (contains ATP/GTP binding P-loop)

Description CEK001CYR_rc /REP_DB=TREMBL Accession /GB=D26651 /CHR=1 /FEA=Genomic Cluster /REVCOMP /DEF=C.elegans cDNA clone yk1c11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 19      6grp_total > 43      4grp_total > 09      6grp_highest_sub > 01_02_03      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21814
1 2 3 4 range
A 25796.2
(-3)
P 		32628.2
(-0.9)
P 		28668.3
(-2.1)
P 		45367
(3)
P 		19571
B 26063.3
(-2.4)
P 		25231
(-3)
P 		27918.9
(-1.2)
P 		34190.8
(3)
P 		8960
C 23553.5
(-3)
P 		25218.4
(-2.4)
P 		23755.4
(-2.9)
P 		41387.7
(3)
P 		17834
avr 25137.7
(-3) 		27692.5
(-2) 		26780.9
(-2.4) 		40315.2
(3) 		15178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6383
1 2 3 4 5 6 range
A 22419.5
(1.1)
P 		21793
(0.3)
P 		23886.1
(3)
P 		20017.7
(-2.1)
P 		19300
(-3)
P 		21188.4
(-0.5)
P 		4586
B 21514.1
(0.8)
P 		20970.9
(0.2)
P 		23880.5
(3)
P 		18958.2
(-1.7)
P 		17569.8
(-3)
P 		17803.6
(-2.8)
P 		6311
C 20235.6
(0.4)
P 		22140.5
(2.7)
P 		22364.3
(3)
P 		19388.9
(-0.7)
P 		20820.2
(1.1)
P 		17502.8
(-3)
P 		4862
avr 21389.7
(0.4) 		21634.8
(0.7) 		23377
(3) 		19454.9
(-2.2) 		19230
(-2.5) 		18831.6
(-3) 		4545
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3161
1 2 3 4 range
A 9392.4
(3)
P 		7082.7
(-2)
P 		7494.4
(-1.1)
P 		6640.8
(-3)
P 		2752
B 8311.4
(3)
P 		6231.5
(-3)
P 		6469.4
(-2.3)
P 		7286.1
(0)
P 		2080
C 7796.9
(0.8)
P 		7250.4
(-1.7)
P 		8279.5
(3)
P 		6978.6
(-3)
P 		1301
avr 8500.2
(3) 		6854.9
(-3) 		7414.4
(-1) 		6968.5
(-2.6) 		1645

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3852
1 2 3 4 5 6 range
A 4132.9
(-3)
P 		7750.7
(3)
P 		5201.9
(-1.2)
P 		5077
(-1.4)
P 		5823.9
(-0.2)
P 		5158.7
(-1.3)
P 		3618
B 7485.9
(3)
P 		6980.3
(1.8)
P 		5798.9
(-1.1)
P 		5021.4
(-3)
P 		7138.4
(2.2)
P 		6638.9
(0.9)
P 		2465
C 7500.5
(3)
P 		6755.7
(1.8)
P 		5692.1
(-0)
P 		3899.1
(-3)
P 		6737.1
(1.7)
P 		5859.2
(0.3)
P 		3601
avr 6373.1
(1.1) 		7162.2
(3) 		5564.3
(-0.8) 		4665.8
(-3) 		6566.5
(1.6) 		5885.6
(-0.1) 		2496
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175073_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28214
Sequence Type Exemplar sequence
Representative Public ID CEK001CYR NCBI
Target Description CEK001CYR_rc /REP_DB=TREMBL Accession /GB=D26651 /CHR=1 /FEA=Genomic Cluster /REVCOMP /DEF=C.elegans cDNA clone yk1c11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060056(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060056 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-2) (eft-2) mRNA, complete cds. 11/11 A 
SNAP00000024319 ENSEMBL cdna:SNAP chromosome:CEL140:I:9171303:9174822:1 11/11 None
GENEFINDER00000024330 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9171596:9174822:1 11/11 None
F25H5.4.2 ENSEMBL cdna:known chromosome:CEL140:I:9171560:9174952:1 gene:F25H5.4 11/11 A 
F25H5.4.3 ENSEMBL cdna:known chromosome:CEL140:I:9171560:9174936:1 gene:F25H5.4 11/11 A 
F25H5.4.1 ENSEMBL cdna:known chromosome:CEL140:I:9171655:9174952:1 gene:F25H5.4 11/11 A 
F25H5.4.4 ENSEMBL cdna:known chromosome:CEL140:I:9171655:9175101:1 gene:F25H5.4 11/11 A 
F25H5.4.6 ENSEMBL cdna:known chromosome:CEL140:I:9171697:9175324:1 gene:F25H5.4 11/11 A 
F25H5.4.5 ENSEMBL cdna:known chromosome:CEL140:I:9171697:9175076:1 gene:F25H5.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9174363-9174782(+) 100.0 100.0
Public Domain and Genome References
Gene Title Elongation factor Tu family (contains ATP/GTP binding P-loop)
Gene Symbol eft-2
Ensembl F25H5.4
Entrez Gene 172743 Entrez gene
SwissProt P29691 EMBL-EBI
WormBase CE15900 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1625150_S_AT Elongation factor 2b dm
DROSGENOME1:143143_AT Elongation factor 2b dm
CHICKEN:GGA.4327.1.S1_AT eukaryotic translation elongation factor 2 gga
HG-U133A:204102_S_AT eukaryotic translation elongation factor 2 hs
HG-U133_PLUS_2:204102_S_AT eukaryotic translation elongation factor 2 hs
HG-FOCUS:204102_S_AT eukaryotic translation elongation factor 2 hs
HG-U133A_2:204102_S_AT eukaryotic translation elongation factor 2 hs
PLASMODIUM_ANOPHELES:200094_S_AT eukaryotic translation elongation factor 2 hs
HG-U133B:200094_S_AT eukaryotic translation elongation factor 2 hs
HG-U133A_2:200094_S_AT eukaryotic translation elongation factor 2 hs
HG-FOCUS:200094_S_AT eukaryotic translation elongation factor 2 hs
HG-U133_PLUS_2:200094_S_AT eukaryotic translation elongation factor 2 hs
HG-U133A:200094_S_AT eukaryotic translation elongation factor 2 hs
U133_X3P:200094_S_AT eukaryotic translation elongation factor 2 hs
HUGENEFL:X51466_AT eukaryotic translation elongation factor 2 hs
U133_X3P:G4503482_3P_A_AT eukaryotic translation elongation factor 2 hs
HG-U95AV2:36587_AT eukaryotic translation elongation factor 2 hs
HG-U95E:87875_AT eukaryotic translation elongation factor 2 hs
MG-U74AV2:97559_AT eukaryotic translation elongation factor 2 mm
MOUSE430A_2:1424736_AT eukaryotic translation elongation factor 2 mm
MOUSE430_2:1424736_AT eukaryotic translation elongation factor 2 mm
MOE430A:1424736_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.17890.0_F_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.16027.0_S_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.600.0_F_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.38636.0_S_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.31964.0_F_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.28607.0_F_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.23588.0_S_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.11057.0_S_AT eukaryotic translation elongation factor 2 mm
MG-U74BV2:164982_AT eukaryotic translation elongation factor 2 mm
MU11KSUBB:MSA.29963.0_F_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU11KSUBB:MSA.41658.0_S_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBA:TC14817_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBA:TC14819_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBA:TC14820_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBA:TC14821_F_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBA:TC24711_F_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
MU19KSUBC:TC38464_F_AT Eukaryotic translation elongation factor 2, mRNA (cDNA clone MGC:6761 IMAGE:3600352) mm
RN-U34:RC_AA963674_AT eukaryotic translation elongation factor 2 rn
RT-U34:RC_AA963674_AT eukaryotic translation elongation factor 2 rn
RG-U34A:RC_AA963674_AT eukaryotic translation elongation factor 2 rn
RN-U34:RC_AA892801_G_AT eukaryotic translation elongation factor 2 rn
RG-U34A:RC_AA892801_G_AT eukaryotic translation elongation factor 2 rn
RG-U34A:RC_AA892801_AT eukaryotic translation elongation factor 2 rn
RG-U34A:RC_AI178750_AT eukaryotic translation elongation factor 2 rn
RAE230A:1370003_AT eukaryotic translation elongation factor 2 rn
RAT230_2:1370003_AT eukaryotic translation elongation factor 2 rn
YEAST_2:1777413_S_AT Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin Sc
YEAST_2:1777413_S_AT Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02985 Hypothetical protein F25H5.4 [Caenorhabditis elegans] ref|NP_492457.1| Elongation FacTor family member (eft-2) [Caenorhabditis elegans] sp|P29691|EF2_CAEEL Elongation factor 2 (EF-2) 0.0
blast AAD03339 elongation factor [Caenorhabditis elegans] 0.0
blast CAE70384 Hypothetical protein CBG16945 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 1.9E-39
Pfam IPR005517 EMBL-EBI Elongation factor G, domain IV 1.6E-50
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 8.2E-15
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.0E-86
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 1.9E-39
Pfam IPR005517 EMBL-EBI Elongation factor G, domain IV 1.6E-50
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 8.2E-15
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.0E-86
Pfam IPR000640 EMBL-EBI Elongation factor G, C-terminal 1.9E-39
Pfam IPR005517 EMBL-EBI Elongation factor G, domain IV 1.6E-50
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 8.2E-15
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.0E-86
Sequence
Target Sequence 
>C. ELEGANS:175073_S_AT
ggacacgttttcgaggagtctcaggtcacaggaactccaatgttcgtcgtcaaggcttac
ttgcctgtcaacgagtccttcggattcaccgccgatctccgctccaacaccggaggacaa
gccttcccacaatgcgtgttcgaccattggcaggtgcttccaggagacccgcttgaggcc
ggaaccaagccaaaccagatcgttttggacaccagaaagagaaaggggctcaaggaaggt
gtcccagccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACACGTTTTCGAGGAGTCTCAGG 327 525 73 Antisense
TCGAGGAGTCTCAGGTCACAGGAAC 88 603 83 Antisense
GTCTCAGGTCACAGGAACTCCAATG 10 471 90 Antisense
GTCACAGGAACTCCAATGTTCGTCG 507 463 97 Antisense
CAATGTTCGTCGTCAAGGCTTACTT 403 183 110 Antisense
GTCGTCAAGGCTTACTTGCCTGTCA 495 473 118 Antisense
AGGCTTACTTGCCTGTCAACGAGTC 431 53 125 Antisense
TGTCAACGAGTCCTTCGGATTCACC 189 559 138 Antisense
CTCCAACACCGGAGGACAAGCCTTC 451 1 174 Antisense
TTGAGGCCGGAACCAAGCCAAACCA 92 547 245 Antisense
GGCTCAAGGAAGGTGTCCCAGCCCT 618 535 299 Antisense
Sequence Source Affymetrix Proprietary Database