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[175055_at] Triosephosphate isomerase

Description CEK113BXR_rc /REP_DB=TREMBL Accession /GB=D73042 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk113b10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 23      6grp_total > 32      4grp_total > 61      6grp_cgc > 61      6grp_high1_sub > 03      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1707
1 2 3 4 range
A 2440.7
(3)
P 		2282
(2.4)
P 		2008.4
(1.5)
P 		753.6
(-3)
P 		1687
B 2455.6
(3)
P 		1584.9
(-0.5)
P 		1670.6
(-0.1)
P 		944.5
(-3)
P 		1511
C 1983.7
(2.7)
P 		1679.9
(1.3)
P 		2042.1
(3)
P 		748.2
(-3)
P 		1294
avr 2293.3
(3) 		1848.9
(1.2) 		1907
(1.4) 		815.4
(-3) 		1478

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1551
1 2 3 4 5 6 range
A 1998.8
(0.6)
P 		2131.3
(1.1)
P 		2627.2
(3)
P 		1974
(0.5)
P 		1866
(0.1)
P 		1076.3
(-3)
P 		1551
B 1957.7
(-1.2)
P 		2461.2
(2.2)
P 		2578.1
(3)
P 		1935.8
(-1.3)
P 		1985.9
(-1)
P 		1691.3
(-3)
P 		887
C 2386.6
(3)
P 		2003.1
(1.2)
P 		2033
(1.3)
P 		2130
(1.8)
P 		1918.1
(0.8)
P 		1102.4
(-3)
P 		1284
avr 2114.4
(1.4) 		2198.5
(1.9) 		2412.8
(3) 		2013.3
(0.9) 		1923.3
(0.4) 		1290
(-3) 		1123
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 778
1 2 3 4 range
A 888.6
(3)
P 		495.4
(-0)
P 		525
(0.2)
P 		110.3
(-3)
P 		778
B 783.1
(3)
P 		391.4
(-1)
P 		387.1
(-1.1)
P 		201.3
(-3)
P 		582
C 656.6
(2.4)
P 		483
(0.7)
P 		711.8
(3)
P 		126.2
(-3)
P 		586
avr 776.1
(3) 		456.6
(-0) 		541.3
(0.8) 		145.9
(-3) 		630

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 623
1 2 3 4 5 6 range
A 368.5
(-1.7)
P 		758
(3)
P 		572.2
(0.8)
P 		500.6
(-0.1)
P 		563.1
(0.6)
P 		262.1
(-3)
P 		496
B 681.2
(0.3)
P 		819.2
(3)
P 		626.1
(-0.8)
P 		512.7
(-3)
P 		806.9
(2.8)
P 		630.7
(-0.7)
P 		307
C 884.6
(3)
P 		611.2
(-0.2)
P 		517.4
(-1.3)
P 		428.3
(-2.3)
P 		620.7
(-0.1)
P 		369
(-3)
P 		516
avr 644.8
(1.4) 		729.5
(3) 		571.9
(-0.1) 		480.5
(-1.8) 		663.6
(1.7) 		420.6
(-3) 		309
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175055_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20632
Sequence Type Exemplar sequence
Representative Public ID CEK113BXR NCBI
Target Description CEK113BXR_rc /REP_DB=TREMBL Accession /GB=D73042 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk113b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064162(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064162 NCBI Caenorhabditis elegans Triose Phosphate Isomerase family member (tpi-1) (tpi-1) mRNA, complete cds. 11/11 None
Y17G7B.7.2 ENSEMBL cdna:known chromosome:CEL140:II:12036740:12039044:-1 gene:Y17G7B.7 11/11 None
Y17G7B.7.1 ENSEMBL cdna:known chromosome:CEL140:II:12036740:12039042:-1 gene:Y17G7B.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12036765-12037104(-) 98.53 100.0
Public Domain and Genome References
Gene Title Triosephosphate isomerase
Gene Symbol tpi-1
Ensembl Y17G7B.7
Entrez Gene 174844 Entrez gene
SwissProt Q10657 EMBL-EBI
WormBase CE19040 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16960_AT triosephosphate isomerase, chloroplast, putative at
ATH1-121501:264018_AT triosephosphate isomerase, chloroplast, putative at
CANINE:1583430_AT similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) cfa
CANINE_2:CFA.6532.1.A1_AT similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) cfa
CANINE_2:CFAAFFX.22441.1.S1_S_AT similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) cfa
DROSOPHILA_2:1637816_S_AT Triose phosphate isomerase dm
DROSGENOME1:152334_AT Triose phosphate isomerase dm
CHICKEN:GGA.4148.1.S1_AT triosephosphate isomerase 1 gga
HG-U95AV2:34003_AT triosephosphate isomerase 1 hs
HG-U133_PLUS_2:200822_X_AT triosephosphate isomerase 1 hs
HG-U133A:200822_X_AT triosephosphate isomerase 1 hs
HG-U133A_2:200822_X_AT triosephosphate isomerase 1 hs
HG-U133A:210050_AT triosephosphate isomerase 1 hs
HG-U133_PLUS_2:210050_AT triosephosphate isomerase 1 hs
HG-U133A_2:210050_AT triosephosphate isomerase 1 hs
U133_X3P:G4507644_3P_A_AT triosephosphate isomerase 1 hs
U133_X3P:G339840_3P_AT triosephosphate isomerase 1 hs
HG-U133A_2:213011_S_AT triosephosphate isomerase 1 hs
HG-FOCUS:213011_S_AT triosephosphate isomerase 1 hs
HG-U133_PLUS_2:213011_S_AT triosephosphate isomerase 1 hs
HG-U133A:213011_S_AT triosephosphate isomerase 1 hs
MG-U74AV2:99566_AT triosephosphate isomerase 1 mm
MOE430A:1415918_A_AT triosephosphate isomerase 1 mm
MOUSE430_2:1415918_A_AT triosephosphate isomerase 1 mm
MOUSE430A_2:1415918_A_AT triosephosphate isomerase 1 mm
MU11KSUBA:AC002393_GENETPI_F_AT triosephosphate isomerase 1 mm
MU11KSUBB:MSA.1619.0_F_AT triosephosphate isomerase 1 mm
MOE430A:1452927_X_AT triosephosphate isomerase 1 mm
MOUSE430A_2:1452927_X_AT triosephosphate isomerase 1 mm
MOUSE430_2:1452927_X_AT triosephosphate isomerase 1 mm
MOE430A:1435659_A_AT triosephosphate isomerase 1 mm
MOUSE430A_2:1435659_A_AT triosephosphate isomerase 1 mm
MOUSE430_2:1435659_A_AT triosephosphate isomerase 1 mm
MU19KSUBA:TC18561_AT Triosephosphate isomerase 1 (Tpi1), mRNA mm
MU19KSUBC:TC39727_F_AT Triosephosphate isomerase 1 (Tpi1), mRNA mm
MU19KSUBC:TC39728_AT Triosephosphate isomerase 1 (Tpi1), mRNA mm
RG-U34A:RC_AI104399_AT triosephosphate isomerase 1 rn
RAE230A:1367603_AT Similar to Tpi1 protein rn
RAT230_2:1367603_AT Similar to Tpi1 protein rn
YEAST_2:1778198_AT Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4807 triose-phosphate isomerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA19447 Hypothetical protein Y17G7B.7 [Caenorhabditis elegans] ref|NP_496563.1| Triose Phosphate Isomerase family member (tpi-1) [Caenorhabditis elegans] sp|Q10657|TPIS_CAEEL Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) 1.0E-126
blast 1MO0 Chain , Structural Genomics Of Caenorhabditis Elegans: Triose Phosphate Isomerase pdb|1MO0|A Chain A, Structural Genomics Of Caenorhabditis Elegans: Triose Phosphate Isomerase 1.0E-126
blast AAA79846 triosephosphate isomerase 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR000652 EMBL-EBI Triosephosphate isomerase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175055_AT
ggttgattttcattgattcccctaatttttagccgtaaaattagctgatttttcccggaa
tttcagcttcaaaattgtagaatttcccttagttttccccaattttcccccagatttcct
ctaaattatattgtaaaattagctgattttcatcaattttcccggaattttgaccgaaaa
aaatccaaaatttgggtgaattcgtgaattttttggcttgaaatttggctttttcctctc
tacagtgtagatattagtagcagcttttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTGATTTTCATTGATTCCCCTAA 356 505 51 Antisense
CATTGATTCCCCTAATTTTTAGCCG 122 213 61 Antisense
TAGCCGTAAAATTAGCTGATTTTTC 341 647 80 Antisense
TGATTTTTCCCGGAATTTCAGCTTC 640 561 96 Antisense
TCAAAATTGTAGAATTTCCCTTAGT 599 627 119 Antisense
TTCCCCCAGATTTCCTCTAAATTAT 61 697 155 Antisense
AGCTGATTTTCATCAATTTTCCCGG 57 81 191 Antisense
TCATCAATTTTCCCGGAATTTTGAC 225 607 200 Antisense
GAATTCGTGAATTTTTTGGCTTGAA 274 323 248 Antisense
TGAAATTTGGCTTTTTCCTCTCTAC 514 569 269 Antisense
GTGTAGATATTAGTAGCAGCTTTTT 150 487 295 Antisense
Sequence Source Affymetrix Proprietary Database