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[175039_at] DNAJ protein

Description CEK084BXR_rc /REP_DB=TREMBL Accession /GB=D71936 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk84b10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 58      6grp_total > 88      4grp_total > 24      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2234
1 2 3 4 range
A 1623.3
(-3)
P 		1942.6
(-1.9)
P 		3082.3
(2)
P 		3359.3
(3)
P 		1736
B 1599.6
(-3)
P 		1678.2
(-2.8)
P 		2689.2
(-0.1)
P 		3833.6
(3)
P 		2234
C 1719.6
(-3)
P 		1707.3
(-3)
P 		2705.7
(0.7)
P 		3318
(3)
P 		1611
avr 1647.5
(-3) 		1776
(-2.6) 		2825.7
(0.8) 		3503.6
(3) 		1856

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2098
1 2 3 4 5 6 range
A 2174
(-3)
P 		2529.7
(-1.4)
P 		2206.7
(-2.9)
P 		2203.5
(-2.9)
P 		3235
(1.7)
P 		3527.9
(3)
P 		1354
B 1860.5
(-3)
P 		2879.9
(0.4)
P 		2166.6
(-2)
P 		2582.7
(-0.6)
P 		2794.9
(0.1)
P 		3661.9
(3)
P 		1801
C 1563.8
(-3)
P 		2678.8
(1.5)
P 		2337.1
(0.1)
P 		2390
(0.3)
P 		2707.9
(1.6)
P 		3063
(3)
P 		1499
avr 1866.1
(-3) 		2696.1
(0.2) 		2236.8
(-1.6) 		2392.1
(-1) 		2912.6
(1) 		3417.6
(3) 		1552
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 529
1 2 3 4 range
A 591.1
(-0.4)
P 		421.7
(-3)
P 		805.8
(3)
P 		491.7
(-1.9)
P 		384
B 510.1
(-1.6)
P 		414.5
(-3)
P 		623.2
(0.1)
P 		816.9
(3)
P 		402
C 569.2
(-2)
P 		490.9
(-3)
P 		943
(3)
P 		559.5
(-2.1)
P 		452
avr 556.8
(-1) 		442.4
(-3) 		790.7
(3) 		622.7
(0.1) 		348

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 965
1 2 3 4 5 6 range
A 400.8
(-3)
P 		899.7
(2.2)
P 		480.6
(-2.2)
P 		558.9
(-1.4)
P 		976.2
(3)
P 		858.9
(1.8)
P 		575
B 647.3
(-2.1)
P 		958.6
(0.1)
P 		526.1
(-3)
P 		684.1
(-1.9)
P 		1135.5
(1.4)
P 		1365.5
(3)
P 		839
C 579.6
(-1.9)
P 		817.4
(0.7)
P 		594.8
(-1.7)
P 		480.6
(-3)
P 		876.2
(1.4)
P 		1025.4
(3)
P 		545
avr 542.6
(-2.9) 		891.9
(0.9) 		533.8
(-3) 		574.5
(-2.6) 		996
(2) 		1083.3
(3) 		550
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175039_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20560
Sequence Type Exemplar sequence
Representative Public ID CEK084BXR NCBI
Target Description CEK084BXR_rc /REP_DB=TREMBL Accession /GB=D71936 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk84b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064067(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064067 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-13) (dnj-13) mRNA, complete cds. 11/11 None
F54D5.8.2 ENSEMBL cdna:known chromosome:CEL140:II:11545604:11547262:1 gene:F54D5.8 11/11 None
F54D5.8.1 ENSEMBL cdna:known chromosome:CEL140:II:11545606:11547262:1 gene:F54D5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11546886-11547232(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ protein
Gene Symbol dnj-13
Ensembl F54D5.8
Entrez Gene 3564910 Entrez gene
SwissProt Q20774 EMBL-EBI
WormBase CE03412 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246894_AT DNAJ heat shock protein, putative at
CANINE:1604150_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
CANINE_2:CFA.16255.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
CANINE_2:CFAAFFX.31168.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
DROSOPHILA_2:1637059_S_AT DnaJ-like-1 dm
DROSGENOME1:143835_AT DnaJ-like-1 dm
CHICKEN:GGA.12894.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
CHICKEN:GGA.13022.1.A1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
CHICKEN:GGAAFFX.22722.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
HG-FOCUS:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A_2:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133_PLUS_2:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95AV2:158_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HC-G110:158_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133_PLUS_2:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A_2:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HU35KSUBA:RC_AA450303_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HUGENEFL:U40992_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
U133_X3P:G6631084_3P_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
U133_X3P:HS.41693.0.S2_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95AV2:33533_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95C:62561_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95E:86876_R_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
MOUSE430A_2:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430A:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430_2:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MU11KSUBB:MSA.27940.0_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MU11KSUBB:MSA.27198.0_F_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MG-U74AV2:93853_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MG-U74BV2:114288_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430A:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430A_2:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430_2:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430B:1439524_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MOUSE430_2:1439524_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBB:TC28401_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBC:TC35461_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBC:TC38135_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
RG-U34B:RC_AI008643_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (predicted) rn
YEAST_2:1776450_AT Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91334 Hypothetical protein F54D5.8 [Caenorhabditis elegans] ref|NP_496468.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-13) [Caenorhabditis elegans] 1.0E-139
blast CAE59478 Hypothetical protein CBG02862 [Caenorhabditis briggsae] 1.0E-126
blast XP_801842 PREDICTED: similar to DnaJ (Hsp40) homolog, subfamily B, member 1 isoform 3 [Strongylocentrotus purpuratus] 1.0E-77
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.00000011306601E-31
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 2.0E-19
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.4E-39
Sequence
Target Sequence 
>C. ELEGANS:175039_AT
tccctctcttgcttatcaatcaactacagtcgaaccaagacgggtaaacaatcactcttg
attgccattggattcgatttagcagtattcagtcgccttgttcgatgttttcaagcgggt
atttaagagattttttattaggatgttgttctttttatcacccatgtttcattgttaatt
acccatctcacacatattgcccatagttattcagatgcattttttatcgtttatcaagtc
tcattctaatgacatgaaaaccttcattgacaagatactcggtgtaattgtatccccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCTCTCTTGCTTATCAATCAACT 697 597 16 Antisense
ATCACTCTTGATTGCCATTGGATTC 606 27 66 Antisense
GATTTAGCAGTATTCAGTCGCCTTG 312 429 91 Antisense
ATTCAGTCGCCTTGTTCGATGTTTT 434 11 102 Antisense
TTCGATGTTTTCAAGCGGGTATTTA 35 689 116 Antisense
GGATGTTGTTCTTTTTATCACCCAT 58 515 156 Antisense
TTATCACCCATGTTTCATTGTTAAT 27 681 170 Antisense
CATTGTTAATTACCCATCTCACACA 435 217 185 Antisense
ATCTCACACATATTGCCCATAGTTA 235 33 200 Antisense
TCGTTTATCAAGTCTCATTCTAATG 185 603 242 Antisense
GATACTCGGTGTAATTGTATCCCCC 10 425 289 Antisense
Sequence Source Affymetrix Proprietary Database