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[175032_at]

Description CEK127BYR_rc /REP_DB=TREMBL Accession /GB=D64883 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk127b11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 91      6grp_high1_10_10 > 49      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 87      6grp_total > 39      4grp_total > 15      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 961
1 2 3 4 range
A 1363.2
(3)
P 		884.9
(-3)
P 		1277.1
(1.9)
P 		1082.2
(-0.5)
P 		478
B 1517.2
(3)
P 		840
(-3)
P 		955.2
(-2)
P 		1024.3
(-1.4)
P 		677
C 1088.3
(-1.8)
P 		976.6
(-2.6)
P 		915.5
(-3)
P 		1800.7
(3)
P 		885
avr 1322.9
(3) 		900.5
(-3) 		1049.3
(-0.9) 		1302.4
(2.7) 		422

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1401
1 2 3 4 5 6 range
A 1534.5
(1.8)
P 		1437.4
(1.4)
P 		514.8
(-3)
P 		979.9
(-0.8)
P 		1585.1
(2.1)
P 		1785.7
(3)
P 		1271
B 1102.4
(1.9)
P 		975.2
(0.9)
P 		480.7
(-3)
P 		1247.6
(3)
P 		1032.5
(1.3)
P 		992.1
(1)
P 		767
C 1881.6
(3)
P 		1000.2
(-1.7)
P 		1176.3
(-0.8)
P 		756.7
(-3)
P 		1502.8
(1)
P 		807.1
(-2.7)
P 		1125
avr 1506.2
(3) 		1137.6
(0.2) 		723.9
(-3) 		994.7
(-0.9) 		1373.5
(2) 		1195
(0.6) 		782
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 338
1 2 3 4 range
A 496.3
(3)
P 		192.1
(-2.4)
P 		333.8
(0.1)
P 		158.4
(-3)
P 		338
B 483.8
(3)
P 		207.5
(-3)
P 		221.3
(-2.7)
P 		218.3
(-2.8)
P 		276
C 360.2
(3)
P 		280.8
(-3)
P 		319.1
(-0.1)
P 		303.6
(-1.3)
P 		79
avr 446.8
(3) 		226.8
(-3) 		291.4
(-1.2) 		226.8
(-3) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 585
1 2 3 4 5 6 range
A 282.9
(-0.4)
P 		511.2
(3)
P 		112.1
(-3)
P 		248.5
(-0.9)
P 		478.3
(2.5)
P 		434.8
(1.9)
P 		399
B 383.6
(2.3)
P 		324.6
(1.1)
P 		116.7
(-3)
P 		330.4
(1.2)
P 		419.5
(3)
P 		370
(2)
P 		303
C 697.4
(3)
P 		305.2
(-1.3)
P 		299.4
(-1.4)
P 		152.2
(-3)
P 		486.3
(0.7)
P 		270.2
(-1.7)
P 		545
avr 454.6
(2.9) 		380.3
(1.3) 		176.1
(-3) 		243.7
(-1.6) 		461.4
(3) 		358.3
(0.8) 		285
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175032_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21380
Sequence Type Exemplar sequence
Representative Public ID CEK127BYR NCBI
Target Description CEK127BYR_rc /REP_DB=TREMBL Accession /GB=D64883 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk127b11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028037(11), NM_001028036(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028037 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
NM_001028036 NCBI Caenorhabditis elegans MiniBrain Kinase (Drosophila) homolog family member (mbk-2) (mbk-2) mRNA, complete cds. 11/11 None
F49E11.1c ENSEMBL cdna:known chromosome:CEL140:IV:13009105:13035916:1 gene:F49E11.1 11/11 A 
F49E11.1b ENSEMBL cdna:known chromosome:CEL140:IV:13009122:13035916:1 gene:F49E11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178250 3 2 NM_001028036 173305_s_at (11), 175032_at (11)
NM_001028037 173305_s_at (11), 175032_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13035419-13035865(+) 100.0 100.0
Public Domain and Genome References
Ensembl F49E11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03528 Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAB03351.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] emb|CAA94353.1| Hypothetical protein F49E11.1b [Caenorhabditis elegans] ref|NP_001023207.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAB54274 Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54308.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] emb|CAB54254.2| Hypothetical protein F49E11.1c [Caenorhabditis elegans] ref|NP_001023208.1| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] gb|AAM09088.1| minibrain kinase 2 [Caenorhabditis elegans] 0.0
blast CAA94352 Hypothetical protein F49E11.1a [Caenorhabditis elegans] ref|NP_502492.2| MiniBrain Kinase (Drosophila) homolog family member (mbk-2) [Caenorhabditis elegans] 0.0
blast CAE56332 Hypothetical protein CBG23998 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-63
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-63
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 2.0E-66
hanks 3.1.4 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk4 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 9.2E-68
Sequence
Target Sequence 
>C. ELEGANS:175032_AT
gacaaccttgtgtaaatatacgatttttgtcatcgttcgcatatagatgtgcaggtcgca
ttctgatcagccactgtctccccaccccactccctcatttcatactaattttcttccctt
gtctctttccatcttctttgcactacatcaaatcactatttttcttagctattgtttgat
atattgttgtcattgtggtgaagctcgtcgcttaaaaagtgacgctgctgaaaccttcac
gacattcttacccatttttccaaatgaacccccctcttcgttaaaaacagacatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAACCTTGTGTAAATATACGATT 562 363 18 Antisense
ATACGATTTTTGTCATCGTTCGCAT 380 23 35 Antisense
ATCGTTCGCATATAGATGTGCAGGT 136 37 49 Antisense
TGTGCAGGTCGCATTCTGATCAGCC 365 557 65 Antisense
CACTCCCTCATTTCATACTAATTTT 25 199 105 Antisense
TTCATACTAATTTTCTTCCCTTGTC 437 687 116 Antisense
TTCCATCTTCTTTGCACTACATCAA 227 701 144 Antisense
GCACTACATCAAATCACTATTTTTC 52 317 157 Antisense
ATCACTATTTTTCTTAGCTATTGTT 68 29 169 Antisense
ACATTCTTACCCATTTTTCCAAATG 533 99 259 Antisense
CCCCTCTTCGTTAAAAACAGACATG 338 261 288 Antisense
Sequence Source Affymetrix Proprietary Database