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[175025_at]

Description CEK116B5R_rc /REP_DB=TREMBL Accession /GB=D73250 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk116b5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 11      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 12      6grp_total > 13      4grp_total > 49      6grp_cgc > 50      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4211
1 2 3 4 range
A 115.5
(-3)
P 		4195.7
(2.9)
P 		4263.4
(3)
P 		119.9
(-3)
P 		4148
B 52.6
(-3)
P 		3906.6
(3)
P 		3833.1
(2.9)
P 		154.8
(-2.8)
P 		3854
C 86.1
(-3)
P 		3897.4
(3)
P 		3803.1
(2.9)
P 		114.1
(-3)
P 		3811
avr 84.7
(-3) 		3999.9
(3) 		3966.5
(2.9) 		129.6
(-2.9) 		3915

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2264
1 2 3 4 5 6 range
A 3152.8
(3)
P 		2395.1
(0.9)
P 		2200.6
(0.3)
P 		2449.4
(1)
P 		1629.6
(-1.3)
P 		1005.3
(-3)
P 		2148
B 3106.9
(3)
P 		2870.6
(2.4)
P 		2441.4
(1.2)
P 		2643.3
(1.7)
P 		1569
(-1.2)
P 		888.7
(-3)
P 		2218
C 2812.4
(3)
P 		2764.4
(2.8)
P 		2159
(0.9)
P 		2392.9
(1.7)
P 		1701.8
(-0.5)
P 		908.5
(-3)
P 		1904
avr 3024
(3) 		2676.7
(2) 		2267
(0.8) 		2495.2
(1.5) 		1633.5
(-1) 		934.2
(-3) 		2090
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1309
1 2 3 4 range
A 42.1
(-2.9)
P 		910.8
(1.9)
P 		1114.5
(3)
P 		17.5
(-3)
P 		1097
B 16.8
(-3)
P 		964.8
(3)
P 		888.2
(2.5)
P 		33
(-2.9)
P 		948
C 28.5
(-3)
P 		1120.5
(2.1)
P 		1325.5
(3)
P 		19.2
(-3)
P 		1306
avr 29.1
(-3) 		998.7
(2.4) 		1109.4
(3) 		23.2
(-3) 		1086

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 836
1 2 3 4 5 6 range
A 581.2
(0.3)
P 		851.8
(3)
P 		479.2
(-0.7)
P 		621.2
(0.7)
P 		491.7
(-0.6)
P 		244.8
(-3)
P 		607
B 1081
(3)
P 		955.5
(2)
P 		592.8
(-0.9)
P 		700.1
(-0)
P 		637.5
(-0.5)
P 		331.4
(-3)
P 		750
C 1042.4
(3)
P 		843.5
(1.4)
P 		549.5
(-1)
P 		481.2
(-1.6)
P 		550.7
(-1)
P 		304.1
(-3)
P 		738
avr 901.5
(3) 		883.6
(2.8) 		540.5
(-0.6) 		600.8
(0) 		560
(-0.4) 		293.4
(-3) 		608
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175025_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20770
Sequence Type Exemplar sequence
Representative Public ID CEK116B5R NCBI
Target Description CEK116B5R_rc /REP_DB=TREMBL Accession /GB=D73250 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk116b5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062965(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062965 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-10) (dpy-10) mRNA, complete cds. 11/11 A 
T14B4.7a.2 ENSEMBL cdna:known chromosome:CEL140:II:6709778:6712174:-1 gene:T14B4.7 11/11 A 
T14B4.7a.1 ENSEMBL cdna:known chromosome:CEL140:II:6710100:6712169:-1 gene:T14B4.7 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182248 5/11 Cross Hyb Matching Probes A 
SNAP00000000073 5/11 Cross Hyb Matching Probes None
GENEFINDER00000000088 5/11 Cross Hyb Matching Probes None
T14B4.7b.1 5/11 Cross Hyb Matching Probes None
T14B4.7b.2 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174106 2 1 NM_062965 175025_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6710119-6710460(-) 98.24 100.0
Public Domain and Genome References
Ensembl T14B4.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495366 DumPY : shorter than wild-type family member (dpy-10) [Caenorhabditis elegans] gb|AAK31556.2| Dumpy : shorter than wild-type protein 10, isoform a [Caenorhabditis elegans] 4.0E-65
blast AAA17395 collagen [Caenorhabditis elegans] sp|P35800|DPY10_CAEEL Cuticle collagen dpy-10 precursor (Dumpy-10 protein) 4.0E-65
blast T28887 collagen dpy-10 - Caenorhabditis elegans 4.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 0.0018
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 1.5E-10
Pfam IPR008160 EMBL-EBI Collagen triple helix repeat 6.9E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495366.2 1 7-29
T14B4.7a.2 1 7-29
T14B4.7a.1 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:175025_AT
attcttttgatcaatccaggaccacagccacgcattcgtgccgatgactacaacagtgat
gacggatatggtggaagtcgtggaggaggagatcgtgccggataccaaggttacggtaga
aagtagtcattcgactttcagatattcttctatcaattaacattaaaaagacctcgatga
cttctcattcaaaatgcgtgtacattgttcatttgtttgtttcccaaatacattcccact
tgcttttagtaatgtttttgactgtttgattctcgtag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTTTTGATCAATCCAGGACCAC 671 9 45 Antisense
CACGCATTCGTGCCGATGACTACAA 215 201 73 Antisense
GCCGATGACTACAACAGTGATGACG 669 283 84 Antisense
GTCGTGGAGGAGGAGATCGTGCCGG 392 473 121 Antisense
GATCGTGCCGGATACCAAGGTTACG 689 295 135 Antisense
GTCATTCGACTTTCAGATATTCTTC 692 467 170 Antisense
AAAAAGACCTCGATGACTTCTCATT 179 129 209 Antisense
GCGTGTACATTGTTCATTTGTTTGT 178 293 240 Antisense
TTGTTTCCCAAATACATTCCCACTT 324 677 261 Antisense
ATACATTCCCACTTGCTTTTAGTAA 395 23 272 Antisense
GTTTTTGACTGTTTGATTCTCGTAG 352 449 298 Antisense
Sequence Source Affymetrix Proprietary Database