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[175023_at]

Description CEK112E8R_rc /REP_DB=TREMBL Accession /GB=D73006 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk112e8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 89      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 82      6grp_total > 99      4grp_total > 0      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 474
1 2 3 4 range
A 359.1
(-3)
P 		443.7
(-1.5)
P 		583
(1)
P 		694.7
(3)
P 		336
B 413
(-2.6)
P 		390.7
(-3)
P 		553.7
(0.2)
P 		697.6
(3)
P 		307
C 464.2
(-1.2)
P 		324.9
(-3)
P 		504.5
(-0.7)
P 		798.5
(3)
P 		474
avr 412.1
(-2.6) 		386.4
(-3) 		547.1
(-0.2) 		730.3
(3) 		344

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 409.6
(0.9)
P 		322.3
(-3)
P 		360.5
(-1.3)
P 		334.6
(-2.4)
P 		456.4
(3)
P 		366.2
(-1)
P 		134
B 371.6
(-1.5)
P 		428.3
(0.2)
P 		323.3
(-3)
P 		363.4
(-1.8)
P 		508.1
(2.6)
P 		522.6
(3)
P 		199
C 355.7
(-1.3)
P 		430.1
(0.5)
P 		306.7
(-2.5)
P 		283.3
(-3)
P 		473
(1.5)
P 		538.6
(3)
P 		255
avr 379
(-1) 		393.6
(-0.4) 		330.2
(-2.9) 		327.1
(-3) 		479.2
(3) 		475.8
(2.9) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 130.7
(0.7)
P 		96.3
(-3)
P 		152.4
(3)
P 		101.7
(-2.4)
P 		56
B 131.7
(1.1)
P 		96.5
(-3)
P 		128.3
(0.7)
P 		148.6
(3)
P 		52
C 153.7
(1.4)
P 		93.4
(-3)
P 		175.8
(3)
P 		134.6
(-0)
P 		82
avr 138.7
(1.6) 		95.4
(-3) 		152.2
(3) 		128.3
(0.5) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 75.5
(-3)
P 		114.6
(0.8)
P 		78.5
(-2.7)
P 		84.9
(-2.1)
P 		137.7
(3)
P 		89.2
(-1.7)
P 		62
B 129.3
(-0.6)
P 		142.6
(0)
P 		78.5
(-3)
P 		96.3
(-2.2)
P 		206.4
(3)
P 		194.9
(2.5)
P 		128
C 131.8
(0.6)
P 		131.2
(0.6)
P 		78.1
(-2)
P 		57
(-3)
P 		153
(1.7)
P 		180.3
(3)
P 		123
avr 112.2
(-0.7) 		129.5
(0.5) 		78.4
(-3) 		79.4
(-2.9) 		165.7
(3) 		154.8
(2.3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175023_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22007
Sequence Type Exemplar sequence
Representative Public ID CEK112E8R NCBI
Target Description CEK112E8R_rc /REP_DB=TREMBL Accession /GB=D73006 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk112e8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028588 NCBI Caenorhabditis elegans DC2.3b (DC2.3) mRNA, complete cds. 11/11 None
SNAP00000026549 ENSEMBL cdna:SNAP chromosome:CEL140:V:204219:209459:1 9/11 None
GENEFINDER00000026555 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:204219:209459:1 9/11 None
DC2.3b ENSEMBL cdna:known chromosome:CEL140:V:201452:209571:1 gene:DC2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178548 2 1 NM_001028588 175023_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:209228-209559(+) 100.0 100.0
Public Domain and Genome References
Ensembl DC2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023759 DC2.3b [Caenorhabditis elegans] gb|AAV28359.1| Hypothetical protein DC2.3b [Caenorhabditis elegans] 0.0
blast NP_001023758 DC2.3a [Caenorhabditis elegans] gb|AAD14724.1| Hypothetical protein DC2.3a [Caenorhabditis elegans] 1.0E-178
blast NP_001023759 DC2.3b [Caenorhabditis elegans] gb|AAV28359.1| Hypothetical protein DC2.3b [Caenorhabditis elegans] 1.0E-171
blast CAE62353 Hypothetical protein CBG06431 [Caenorhabditis briggsae] 1.0E-159
blast NP_001023758 DC2.3a [Caenorhabditis elegans] gb|AAD14724.1| Hypothetical protein DC2.3a [Caenorhabditis elegans] 9.0E-98
blast NP_001023758 DC2.3a [Caenorhabditis elegans] gb|AAD14724.1| Hypothetical protein DC2.3a [Caenorhabditis elegans] 4.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 1.0E-25
Pfam IPR007233 EMBL-EBI Sybindin-like protein 1.5E-20
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 1.0E-25
Sequence
Target Sequence 
>C. ELEGANS:175023_AT
ttcggaacattcccatttcacaaaggaatcacccatgacattgtgttcaccgcctatgga
aaatctgttacagtcgacgtggatggggcgccatttgtgaagttcgtttaccgagatggc
gatgatccggtgaacgtcgatcagattactgtagtcggagatgtattgattcatcgattc
gagcacaagggatgacctattttttgaccatttata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGGAACATTCCCATTTCACAAAG 354 693 49 Antisense
TCACCCATGACATTGTGTTCACCGC 97 627 77 Antisense
GTGTTCACCGCCTATGGAAAATCTG 458 485 91 Antisense
TTACAGTCGACGTGGATGGGGCGCC 481 657 116 Antisense
ATGGGGCGCCATTTGTGAAGTTCGT 38 51 131 Antisense
GAAGTTCGTTTACCGAGATGGCGAT 432 337 147 Antisense
AGATGGCGATGATCCGGTGAACGTC 329 67 162 Antisense
GGTGAACGTCGATCAGATTACTGTA 521 495 177 Antisense
ATGTATTGATTCATCGATTCGAGCA 58 49 209 Antisense
GATTCGAGCACAAGGGATGACCTAT 252 431 224 Antisense
ATGACCTATTTTTTGACCATTTATA 223 45 240 Antisense
Sequence Source Affymetrix Proprietary Database