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[174988_at] RAS-related protein

Description CEK115B5R_rc /REP_DB=TREMBL Accession /GB=D73176 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk115b5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 0      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 46      6grp_total > 09      4grp_total > 02      6grp_cgc > 0      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2839
1 2 3 4 range
A 2748.9
(-2.5)
P 		2512.4
(-3)
P 		2575.1
(-2.9)
P 		5105.3
(3)
P 		2593
B 2828
(-2.1)
P 		2429.7
(-3)
P 		2522.4
(-2.8)
P 		4994.4
(3)
P 		2565
C 2477.1
(-2.7)
P 		3040.2
(-1.5)
P 		2351.4
(-3)
P 		5190.3
(3)
P 		2839
avr 2684.7
(-2.5) 		2660.8
(-2.6) 		2483
(-3) 		5096.7
(3) 		2614

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2305
1 2 3 4 5 6 range
A 4146.4
(3)
P 		2445.8
(-1.8)
P 		2362
(-2)
P 		3134.4
(0.1)
P 		2958.8
(-0.4)
P 		2025.5
(-3)
P 		2121
B 3006.1
(1.8)
P 		2544.8
(-0.4)
P 		2404.5
(-1)
P 		3262.3
(3)
P 		2661.4
(0.2)
P 		1979.9
(-3)
P 		1282
C 2677.2
(0.3)
P 		2306.9
(-1.2)
P 		2825.2
(0.9)
P 		3361.8
(3)
P 		2627
(0.1)
P 		1841.6
(-3)
P 		1520
avr 3276.6
(3) 		2432.5
(-0.8) 		2530.6
(-0.4) 		3252.8
(2.9) 		2749.1
(0.6) 		1949
(-3) 		1328
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 1000.9
(3)
P 		545.4
(-3)
P 		673.2
(-1.3)
P 		747.3
(-0.3)
P 		456
B 901.8
(1)
P 		600.1
(-2.8)
P 		584.5
(-3)
P 		1064.3
(3)
P 		480
C 820
(-2.9)
P 		874.1
(2.9)
P 		819.5
(-3)
P 		875.2
(3)
P 		56
avr 907.6
(3) 		673.2
(-3) 		692.4
(-2.5) 		895.6
(2.7) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 588
1 2 3 4 5 6 range
A 764.4
(1.1)
P 		869.8
(2.7)
P 		514.4
(-2.7)
P 		795
(1.5)
P 		892.8
(3)
P 		493.1
(-3)
P 		400
B 1046
(2.6)
P 		847.1
(0.2)
P 		583.9
(-3)
P 		864.1
(0.4)
P 		1081.3
(3)
P 		738.3
(-1.1)
P 		497
C 992.3
(3)
P 		703.9
(-1.6)
P 		719.1
(-1.4)
P 		676.1
(-2)
P 		850
(0.7)
P 		616.5
(-3)
P 		376
avr 934.2
(2.9) 		806.9
(0.6) 		605.8
(-3) 		778.4
(0.1) 		941.4
(3) 		616
(-2.8) 		336
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174988_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22297
Sequence Type Exemplar sequence
Representative Public ID CEK115B5R NCBI
Target Description CEK115B5R_rc /REP_DB=TREMBL Accession /GB=D73176 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk115b5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078171(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078171 NCBI Caenorhabditis elegans RAB family member (rab-14) (rab-14) mRNA, complete cds. 11/11 None
K09A9.2.2 ENSEMBL cdna:known chromosome:CEL140:X:15604683:15606555:-1 gene:K09A9.2 11/11 None
K09A9.2.1 ENSEMBL cdna:known chromosome:CEL140:X:15604683:15606553:-1 gene:K09A9.2 11/11 None
K09A9.2.3 ENSEMBL cdna:known chromosome:CEL140:X:15604683:15606552:-1 gene:K09A9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15604705-15605036(-) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-related protein
Gene Symbol rab-14
Ensembl K09A9.2
Entrez Gene 181649 Entrez gene
SwissProt Q93874 EMBL-EBI
WormBase CE11974 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604788_S_AT similar to RAB14, member RAS oncogene family cfa
CANINE:1587160_AT similar to RAB14, member RAS oncogene family cfa
CANINE_2:CFA.13770.1.S1_S_AT similar to RAB14, member RAS oncogene family cfa
CANINE_2:CFA.756.1.S1_AT similar to RAB14, member RAS oncogene family cfa
DROSOPHILA_2:1623737_A_AT Rab-protein 14 dm
DROSGENOME1:154487_AT Rab-protein 14 dm
CHICKEN:GGA.16164.1.S1_AT RAB14, member RAS oncogene family gga
CHICKEN:GGA.16164.1.S1_S_AT RAB14, member RAS oncogene family gga
CHICKEN:GGA.17303.1.S1_S_AT RAB14, member RAS oncogene family gga
HG-U133_PLUS_2:211503_S_AT RAB14, member RAS oncogene family hs
HG-U133A:211503_S_AT RAB14, member RAS oncogene family hs
HG-FOCUS:211503_S_AT RAB14, member RAS oncogene family hs
HG-U133A_2:211503_S_AT RAB14, member RAS oncogene family hs
HG-U133_PLUS_2:200928_S_AT RAB14, member RAS oncogene family hs
HG-U133A:200928_S_AT RAB14, member RAS oncogene family hs
HG-U133A_2:200928_S_AT RAB14, member RAS oncogene family hs
HU35KSUBA:AA018804_AT RAB14, member RAS oncogene family hs
HU35KSUBA:N23817_AT RAB14, member RAS oncogene family hs
HU35KSUBA:RC_AA403162_AT RAB14, member RAS oncogene family hs
HU35KSUBB:RC_AA088698_AT RAB14, member RAS oncogene family hs
HU35KSUBC:RC_T66955_AT RAB14, member RAS oncogene family hs
HU35KSUBD:RC_Z39978_AT RAB14, member RAS oncogene family hs
HU35KSUBD:RC_T11252_AT RAB14, member RAS oncogene family hs
U133_X3P:G7328165_3P_A_AT RAB14, member RAS oncogene family hs
U133_X3P:G6563199_3P_S_AT RAB14, member RAS oncogene family hs
U133_X3P:HS.5807.0.S2_3P_A_AT RAB14, member RAS oncogene family hs
HG-U133A_2:200927_S_AT RAB14, member RAS oncogene family hs
HG-U133_PLUS_2:200927_S_AT RAB14, member RAS oncogene family hs
HG-U133A:200927_S_AT RAB14, member RAS oncogene family hs
HG-U95AV2:35325_AT RAB14, member RAS oncogene family hs
HG-U95B:46027_AT RAB14, member RAS oncogene family hs
MOE430A:1419246_S_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1419246_S_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1419246_S_AT RAB14, member RAS oncogene family mm
MOE430A:1419245_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1419245_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1419245_AT RAB14, member RAS oncogene family mm
MOE430A:1419243_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1419243_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1419243_AT RAB14, member RAS oncogene family mm
MG-U74AV2:104442_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1419244_A_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1419244_A_AT RAB14, member RAS oncogene family mm
MOE430A:1419244_A_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1439687_AT RAB14, member RAS oncogene family mm
MOE430B:1439687_AT RAB14, member RAS oncogene family mm
MOE430B:1415686_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1415686_AT RAB14, member RAS oncogene family mm
MOE430A:1415686_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1415686_AT RAB14, member RAS oncogene family mm
MU11KSUBA:AA597263_AT RAB14, member RAS oncogene family mm
MU11KSUBB:MSA.16942.0_F_AT RAB14, member RAS oncogene family mm
MU11KSUBB:MSA.5955.0_S_AT RAB14, member RAS oncogene family mm
MU11KSUBB:MSA.37045.0_S_AT RAB14, member RAS oncogene family mm
MG-U74AV2:95686_AT RAB14, member RAS oncogene family mm
MG-U74AV2:97301_AT RAB14, member RAS oncogene family mm
MG-U74BV2:105420_AT RAB14, member RAS oncogene family mm
MOE430A:1441992_AT RAB14, member RAS oncogene family mm
MOUSE430A_2:1441992_AT RAB14, member RAS oncogene family mm
MOUSE430_2:1441992_AT RAB14, member RAS oncogene family mm
MG-U74BV2:108294_AT RAB14, member RAS oncogene family, mRNA (cDNA clone MGC:36272 IMAGE:3980228) mm
MU11KSUBA:AA388963_S_AT RAB14, member RAS oncogene family, mRNA (cDNA clone MGC:36272 IMAGE:3980228) mm
MU19KSUBC:TC39996_AT RAB14, member RAS oncogene family, mRNA (cDNA clone MGC:36272 IMAGE:3980228) mm
RG-U34A:M83680_AT RAB14, member RAS oncogene family rn
RG-U34C:RC_AI172532_AT RAB14, member RAS oncogene family rn
RAE230A:1367884_AT RAB14, member RAS oncogene family rn
RAT230_2:1367884_AT RAB14, member RAS oncogene family rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01884 Hypothetical protein K09A9.2 [Caenorhabditis elegans] ref|NP_510572.1| RAB family member (rab-14) [Caenorhabditis elegans] 1.0E-119
blast CAE63333 Hypothetical protein CBG07733 [Caenorhabditis briggsae] 1.0E-118
blast XP_797260 PREDICTED: similar to RAB14, member RAS oncogene family [Strongylocentrotus purpuratus] 4.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.1E-91
Sequence
Target Sequence 
>C. ELEGANS:174988_AT
ttccaaattctagttgcctgtcaaaatctaaacttgtaatcactataaaactactctgaa
attcagaactttgtgtcacttttacttaccaattcccagtctttttcagtcccccaccca
ttactcactatcatcatcgaactgtgaaccgttcataatataacactttcgctcacccaa
ccaactgcatatttagtagattttttctgtcaaaaaccgttttgaacatttttcccccat
tccttgtgtgataagaaacatctatatgatcccaccccattaagtgctataa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCAAATTCTAGTTGCCTGTCAAA 586 699 13 Antisense
AATTCAGAACTTTGTGTCACTTTTA 217 177 72 Antisense
TCACTTTTACTTACCAATTCCCAGT 142 627 88 Antisense
TTACCAATTCCCAGTCTTTTTCAGT 295 679 98 Antisense
ACCCATTACTCACTATCATCATCGA 351 91 128 Antisense
TATCATCATCGAACTGTGAACCGTT 530 659 141 Antisense
CATAATATAACACTTTCGCTCACCC 654 213 166 Antisense
CTCACCCAACCAACTGCATATTTAG 564 227 184 Antisense
TTCCCCCATTCCTTGTGTGATAAGA 149 699 244 Antisense
AGAAACATCTATATGATCCCACCCC 234 73 266 Antisense
GATCCCACCCCATTAAGTGCTATAA 405 415 280 Antisense
Sequence Source Affymetrix Proprietary Database