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[174987_at]

Description CEK039BXR_rc /REP_DB=TREMBL Accession /GB=D33977 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk39b10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 30      6grp_total > 27      4grp_total > 31      6grp_highest_sub > De      6grp_high1_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 962
1 2 3 4 range
A 1327
(3)
P 		473.5
(-3)
P 		545.2
(-2.5)
P 		858.6
(-0.3)
P 		854
B 1113.8
(3)
P 		507.7
(-1.9)
P 		365.3
(-3)
P 		748.4
(0.1)
P 		749
C 1187.5
(3)
P 		605.6
(-3)
P 		681.5
(-2.2)
P 		762.6
(-1.4)
P 		582
avr 1209.4
(3) 		528.9
(-3) 		530.7
(-3) 		789.9
(-0.7) 		681

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1063
1 2 3 4 5 6 range
A 1147.6
(3)
P 		1007.6
(1.5)
P 		599.9
(-3)
P 		670.5
(-2.2)
P 		754.9
(-1.3)
P 		670
(-2.2)
P 		548
B 1537.6
(3)
P 		1008.5
(0)
P 		474.5
(-3)
P 		935.5
(-0.4)
P 		590.7
(-2.3)
P 		794.6
(-1.2)
P 		1063
C 1225.8
(3)
P 		1038.5
(1.1)
P 		677.4
(-2.7)
P 		669.7
(-2.7)
P 		643.9
(-3)
P 		794.8
(-1.4)
P 		582
avr 1303.7
(3) 		1018.2
(0.6) 		583.9
(-3) 		758.6
(-1.5) 		663.2
(-2.3) 		753.1
(-1.6) 		720
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 399
1 2 3 4 range
A 483.2
(3)
P 		102.8
(-3)
P 		142.5
(-2.4)
P 		125.7
(-2.6)
P 		380
B 355.2
(3)
P 		125.4
(-2.1)
P 		84.7
(-3)
P 		159.5
(-1.3)
P 		271
C 393.1
(3)
P 		174.1
(-2)
P 		237.5
(-0.5)
P 		128.6
(-3)
P 		265
avr 410.5
(3) 		134.1
(-3) 		154.9
(-2.5) 		137.9
(-2.9) 		276

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 420
1 2 3 4 5 6 range
A 211.5
(-0.9)
P 		358.4
(3)
P 		130.6
(-3)
P 		170
(-2)
P 		227.8
(-0.4)
P 		163.1
(-2.1)
P 		228
B 535
(3)
P 		335.7
(0.2)
P 		115.2
(-3)
P 		247.8
(-1.1)
P 		240
(-1.2)
P 		296.3
(-0.4)
P 		420
C 454.4
(3)
P 		316.9
(0.4)
P 		172.4
(-2.3)
P 		134.7
(-3)
P 		208.4
(-1.6)
P 		266.1
(-0.5)
P 		320
avr 400.3
(3) 		337
(1.5) 		139.4
(-3) 		184.2
(-2) 		225.4
(-1) 		241.8
(-0.6) 		261
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174987_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20673
Sequence Type Exemplar sequence
Representative Public ID CEK039BXR NCBI
Target Description CEK039BXR_rc /REP_DB=TREMBL Accession /GB=D33977 /CHR=2 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk39b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062709(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062709 NCBI Caenorhabditis elegans C25H3.8 (C25H3.8) mRNA, complete cds. 11/11 None
C25H3.8 ENSEMBL cdna:known chromosome:CEL140:II:5669745:5678209:-1 gene:C25H3.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5669762-5670152(-) 96.92 100.0
Public Domain and Genome References
Gene Title
Ensembl C25H3.8
Entrez Gene 173962 Entrez gene
SwissProt Q18183 EMBL-EBI
WormBase CE32142 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.12200.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.26156.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.26156.3.S1_S_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.2669.1.S1_AT similar to vacuolar protein sorting 13D gga
CHICKEN:GGAAFFX.2741.1.S1_AT similar to vacuolar protein sorting 13D gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4420 hydroxymethylglutaryl-CoA reductase (NADPH) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31453 Hypothetical protein C25H3.8 [Caenorhabditis elegans] ref|NP_495110.2| C25H3.8 [Caenorhabditis elegans] 0.0
blast T15626 hypothetical protein C25H3.8 - Caenorhabditis elegans 0.0
blast CAE59168 Hypothetical protein CBG02475 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174987_AT
acgtagacccgaatagttttgacatttatcacacatttatcactttcttccttacttttt
taattttttttttctaacaccggtttttaatacctgaaatgggctagtctttccttctta
ttttgattaggaaatgatgatctactactttggatcgttcttaattatttttcttatcgt
gatgctttacaatttctaccctcatctttatttcatccccctgttattcctgtgataaga
tttttctcatacttggttcagttccttaattggttttttttgctgggaaaacaattcgct
ttgactcttaaatcgtgtatgcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTAGACCCGAATAGTTTTGACAT 467 91 21 Antisense
ATCACACATTTATCACTTTCTTCCT 678 27 48 Antisense
GAAATGGGCTAGTCTTTCCTTCTTA 144 361 116 Antisense
TCTACTACTTTGGATCGTTCTTAAT 113 615 161 Antisense
TATTTTTCTTATCGTGATGCTTTAC 538 661 186 Antisense
GATGCTTTACAATTTCTACCCTCAT 480 413 201 Antisense
TCCCCCTGTTATTCCTGTGATAAGA 437 597 236 Antisense
AGATTTTTCTCATACTTGGTTCAGT 700 63 258 Antisense
TACTTGGTTCAGTTCCTTAATTGGT 410 645 270 Antisense
GGAAAACAATTCGCTTTGACTCTTA 142 527 306 Antisense
TTGACTCTTAAATCGTGTATGCTTG 562 697 321 Antisense
Sequence Source Affymetrix Proprietary Database