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[174977_at]

Description CEK044GXR_rc /REP_DB=TREMBL Accession /GB=D34347 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk44g10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 75      6grp_total > 36      4grp_total > 39      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 930
1 2 3 4 range
A 1220.8
(3)
P 		611.9
(-1.7)
P 		444.8
(-3)
P 		503.1
(-2.5)
P 		776
B 1363.9
(3)
P 		474.3
(-3)
P 		695.8
(-1.5)
P 		800.3
(-0.8)
P 		890
C 1374.9
(3)
P 		499.6
(-3)
P 		627.3
(-2.1)
P 		941.1
(0)
P 		875
avr 1319.9
(3) 		528.6
(-3) 		589.3
(-2.5) 		748.2
(-1.3) 		791

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 714
1 2 3 4 5 6 range
A 1274
(3)
P 		904.6
(-1.5)
P 		833.3
(-2.3)
P 		779.2
(-3)
P 		813.8
(-2.6)
P 		898.4
(-1.6)
P 		495
B 898.3
(-0.2)
P 		1182.6
(3)
P 		942.9
(0.3)
P 		951.8
(0.4)
P 		655.2
(-3)
P 		1114.5
(2.2)
P 		527
C 1344.9
(3)
P 		1238.7
(2.1)
P 		725.1
(-2.2)
P 		631.1
(-3)
P 		680.8
(-2.6)
P 		973
(-0.1)
P 		714
avr 1172.4
(3) 		1108.6
(2.2) 		833.8
(-1.5) 		787.4
(-2.1) 		716.6
(-3) 		995.3
(0.7) 		456
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 444.5
(3)
P 		132.8
(-2)
P 		116.3
(-2.3)
P 		73.6
(-3)
P 		371
B 434.9
(3)
P 		117.1
(-3)
P 		161.2
(-2.2)
P 		170.5
(-2)
P 		318
C 455.1
(3)
P 		143.6
(-3)
P 		218.6
(-1.6)
P 		158.7
(-2.7)
P 		312
avr 444.8
(3) 		131.2
(-3) 		165.4
(-2.3) 		134.3
(-2.9) 		314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 372
1 2 3 4 5 6 range
A 234.8
(-0.7)
P 		321.7
(3)
P 		181.5
(-3)
P 		197.6
(-2.3)
P 		245.6
(-0.3)
P 		218.7
(-1.4)
P 		140
B 312.6
(-0.3)
P 		393.6
(2.3)
P 		229
(-3)
P 		252.1
(-2.3)
P 		266.2
(-1.8)
P 		415.6
(3)
P 		187
C 498.5
(3)
P 		378
(1.1)
P 		184.6
(-2.1)
P 		126.9
(-3)
P 		220.3
(-1.5)
P 		325.7
(0.2)
P 		372
avr 348.6
(2.4) 		364.4
(3) 		198.4
(-2.8) 		192.2
(-3) 		244
(-1.2) 		320
(1.5) 		172
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174977_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20275
Sequence Type Exemplar sequence
Representative Public ID CEK044GXR NCBI
Target Description CEK044GXR_rc /REP_DB=TREMBL Accession /GB=D34347 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk44g10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059151(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059151 NCBI Caenorhabditis elegans LEThal family member (let-363) (let-363) mRNA, complete cds. 11/11 None
B0261.2b ENSEMBL cdna:known chromosome:CEL140:I:5245746:5263427:1 gene:B0261.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172167 2 1 NM_059151 174977_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5263017-5263392(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0261.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84886 Lethal protein 363, isoform b [Caenorhabditis elegans] ref|NP_491552.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast AAN84885 Lethal protein 363, isoform a [Caenorhabditis elegans] ref|NP_491549.2| LEThal family member (let-363) [Caenorhabditis elegans] 0.0
blast Q95Q95 Target of rapamycin homolog (CeTOR) (Lethal protein 363) pir||T25444 hypothetical protein B0261.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 1.1E-104
Pfam IPR003152 EMBL-EBI PIK-related kinase, FATC 3.8E-21
Pfam IPR003151 EMBL-EBI PIK-related kinase, FAT 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:174977_AT
agatatactgtttctattgtagattgcgaaatttcgtataacatttctctccttactgac
cccccgttttccttgtttgccatcagccaaaaaaaaactaaaaaactttccagtcaaaca
ttccaagacttttatgctcaacaaatttatttatctctacgatattgtttcggttcttaa
atttctcgtatacgtttgccatgacccaaaatttatttttttctgtttttcgtattttaa
atatttttcattataccctttcttccttcacttcgtttttctctcacagcaataa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATATACTGTTTCTATTGTAGATT 623 65 27 Antisense
TCGTATAACATTTCTCTCCTTACTG 555 603 60 Antisense
TTTTCCTTGTTTGCCATCAGCCAAA 60 677 93 Antisense
TTCCAGTCAAACATTCCAAGACTTT 647 699 134 Antisense
ATTCCAAGACTTTTATGCTCAACAA 478 7 146 Antisense
GCTCAACAAATTTATTTATCTCTAC 467 301 162 Antisense
ATCTCTACGATATTGTTTCGGTTCT 103 31 179 Antisense
TCGGTTCTTAAATTTCTCGTATACG 205 607 196 Antisense
TTTCTCGTATACGTTTGCCATGACC 581 671 208 Antisense
ATACGTTTGCCATGACCCAAAATTT 591 21 216 Antisense
CTTCGTTTTTCTCTCACAGCAATAA 171 221 297 Antisense
Sequence Source Affymetrix Proprietary Database