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[174966_at] long-chain-fatty-acid coA ligase

Description CEK131C5R_rc /REP_DB=TREMBL Accession /GB=D65111 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk131c5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 67      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 88      6grp_total > 67      4grp_total > 04      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3140
1 2 3 4 range
A 1391.4
(-0.9)
P 		538.9
(-3)
P 		586.2
(-2.9)
P 		3018.8
(3)
P 		2480
B 1223.1
(-1.7)
P 		814.7
(-2.5)
P 		564.4
(-3)
P 		3678.9
(3)
P 		3115
C 1310
(-1.7)
P 		821.3
(-2.7)
P 		676.4
(-3)
P 		3604.6
(3)
P 		2928
avr 1308.2
(-1.5) 		725
(-2.8) 		609
(-3) 		3434.1
(3) 		2825

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1581
1 2 3 4 5 6 range
A 976.8
(-1.7)
P 		753.8
(-2.8)
P 		1112.9
(-1)
P 		708
(-3)
P 		1117.9
(-1)
P 		1914.6
(3)
P 		1207
B 1208.4
(-1.9)
P 		999.8
(-2.8)
P 		1325
(-1.4)
P 		960.7
(-3)
P 		969.3
(-3)
P 		2289.1
(3)
P 		1328
C 1094.3
(-2)
P 		1056.3
(-2.2)
P 		912.3
(-2.9)
P 		1152.2
(-1.7)
P 		902.7
(-3)
P 		2018.8
(3)
P 		1116
avr 1093.2
(-2.2) 		936.6
(-3) 		1116.7
(-2.1) 		940.3
(-3) 		996.6
(-2.7) 		2074.2
(3) 		1138
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 667
1 2 3 4 range
A 506.6
(3)
P 		117
(-3)
P 		153.3
(-2.4)
P 		441.9
(2)
P 		390
B 390.1
(-0.6)
P 		201.2
(-2.4)
P 		130.8
(-3)
P 		784
(3)
P 		653
C 433.6
(0.2)
P 		236.1
(-3)
P 		235.8
(-3)
P 		607.8
(3)
P 		372
avr 443.4
(0.7) 		184.8
(-2.8) 		173.3
(-3) 		611.2
(3) 		438

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 674
1 2 3 4 5 6 range
A 180.1
(-3)
P 		268.1
(-1.1)
P 		242.4
(-1.7)
P 		179.6
(-3)
P 		337.3
(0.3)
P 		466.1
(3)
P 		287
B 420.5
(-1.3)
P 		332.8
(-2.2)
P 		321.8
(-2.3)
P 		254.4
(-3)
P 		393.8
(-1.6)
P 		853.6
(3)
P 		599
C 405.6
(-0.7)
P 		322.3
(-1.8)
P 		232.2
(-3)
P 		231.7
(-3)
P 		292.1
(-2.2)
P 		675.8
(3)
P 		444
avr 335.4
(-1.5) 		307.7
(-1.8) 		265.5
(-2.4) 		221.9
(-3) 		341.1
(-1.4) 		665.2
(3) 		443
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174966_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22039
Sequence Type Exemplar sequence
Representative Public ID CEK131C5R NCBI
Target Description CEK131C5R_rc /REP_DB=TREMBL Accession /GB=D65111 /CHR=X /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk131c5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076592(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076592 NCBI Caenorhabditis elegans fatty Acid CoA Synthetase family member (acs-17) (acs-17) mRNA, complete cds. 11/11 None
C46F4.2.2 ENSEMBL cdna:known chromosome:CEL140:X:5937538:5942005:-1 gene:C46F4.2 11/11 None
C46F4.2.1 ENSEMBL cdna:known chromosome:CEL140:X:5937538:5942153:-1 gene:C46F4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5937547-5937881(-) 100.0 100.0
Public Domain and Genome References
Gene Title long-chain-fatty-acid coA ligase
Gene Symbol acs-17
Ensembl C46F4.2
Entrez Gene 180859 Entrez gene
SwissProt Q18660 EMBL-EBI
WormBase CE30907 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:20390_S_AT long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein (LACS8) at
ATH1-121501:263811_AT long-chain-fatty-acid--CoA ligase family protein / long-chain acyl-CoA synthetase family protein (LACS8) at
CANINE:1596168_AT similar to acyl-CoA synthetase long-chain family member 4 isoform 2 cfa
CANINE_2:CFAAFFX.27683.1.S1_S_AT similar to acyl-CoA synthetase long-chain family member 4 isoform 2 cfa
CHICKEN:GGA.11207.1.S1_S_AT acyl-CoA synthetase long-chain family member 4 gga
CHICKEN:GGAAFFX.22457.1.S1_S_AT acyl-CoA synthetase long-chain family member 4 gga
HG-U133A:202422_S_AT acyl-CoA synthetase long-chain family member 4 hs
HG-FOCUS:202422_S_AT acyl-CoA synthetase long-chain family member 4 hs
HG-U133A_2:202422_S_AT acyl-CoA synthetase long-chain family member 4 hs
HG-U133_PLUS_2:202422_S_AT acyl-CoA synthetase long-chain family member 4 hs
HU35KSUBB:RC_AA069611_AT acyl-CoA synthetase long-chain family member 4 hs
HU35KSUBC:RC_AA251792_AT acyl-CoA synthetase long-chain family member 4 hs
HU35KSUBD:RC_H67886_AT acyl-CoA synthetase long-chain family member 4 hs
U133_X3P:G12669908_3P_A_AT acyl-CoA synthetase long-chain family member 4 hs
HG-U95AV2:38099_R_AT acyl-CoA synthetase long-chain family member 4 hs
U133_X3P:HS2.375689.1.S1_3P_X_AT Acyl-CoA synthetase long-chain family member 4 hs
U133_X3P:HS2.375689.1.S1_3P_AT Acyl-CoA synthetase long-chain family member 4 hs
U133_X3P:HS2.269559.1.S1_3P_X_AT Acyl-CoA synthetase long-chain family member 4 hs
U133_X3P:1559663_3P_AT Acyl-CoA synthetase long-chain family member 4 hs
HG-U133_PLUS_2:1557418_AT Acyl-CoA synthetase long-chain family member 4 hs
HG-U133_PLUS_2:1557419_A_AT Acyl-CoA synthetase long-chain family member 4 hs
HG-U133_PLUS_2:1559663_AT Acyl-CoA synthetase long-chain family member 4 hs
HG-U95E:67487_AT Acyl-CoA synthetase long-chain family member 4 hs
HG-U95E:78935_AT Acyl-CoA synthetase long-chain family member 4 hs
MOE430A:1451828_A_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430A_2:1451828_A_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430_2:1451828_A_AT acyl-CoA synthetase long-chain family member 4 mm
MG-U74BV2:105803_AT acyl-CoA synthetase long-chain family member 4 mm
MG-U74AV2:104017_AT acyl-CoA synthetase long-chain family member 4 mm
MG-U74AV2:102381_AT acyl-CoA synthetase long-chain family member 4 mm
MOE430A:1433531_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430A_2:1433531_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430_2:1433531_AT acyl-CoA synthetase long-chain family member 4 mm
MOE430A:1418911_S_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430A_2:1418911_S_AT acyl-CoA synthetase long-chain family member 4 mm
MOUSE430_2:1418911_S_AT acyl-CoA synthetase long-chain family member 4 mm
MU19KSUBB:TC34819_AT Acyl-CoA synthetase long-chain family member 4, transcript variant 2, mRNA (cDNA clone MGC:5674 IMAGE:3157957) mm
RG-U34A:D85189_AT acyl-CoA synthetase long-chain family member 4 rn
RG-U34A:RC_AI236284_S_AT acyl-CoA synthetase long-chain family member 4 rn
RAE230A:1387101_AT acyl-CoA synthetase long-chain family member 4 rn
RAT230_2:1387101_AT acyl-CoA synthetase long-chain family member 4 rn
RG-U34A:RC_AI045002_AT Acyl-CoA synthetase long-chain family member 4 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_508993 fatty Acid CoA Synthetase family member (acs-17) [Caenorhabditis elegans] gb|AAA80409.2| Fatty acid coa synthetase family protein 17 [Caenorhabditis elegans] 0.0
blast CAE68824 Hypothetical protein CBG14783 [Caenorhabditis briggsae] 0.0
blast AAC48292 Hypothetical protein F37C12.7 [Caenorhabditis elegans] ref|NP_498568.1| F37C12.7 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 3.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.4E-98
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508993.2 1 20-42
C46F4.2.2 1 20-42
C46F4.2.1 1 20-42
Sequence
Target Sequence 
>C. ELEGANS:174966_AT
aacgcattctaaatacttaatctgttttgatgtagtctagtatagaatgtacaatattct
cctttcatttctctttcacagaaatcttgctcattgtgtgccttcatttcgcagtttaaa
tatttttatcactcttattttccaagaatcaaatcctttttaccgaatcacaaatcgacg
cggtggccatatgttttagggtattctgatttccacgcttttaatggaaagaaatctgct
cttgtttgtttttttttgttcttttcaaaatttaatttgtgtataacgttctcgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGCATTCTAAATACTTAATCTGT 596 145 20 Antisense
GTACAATATTCTCCTTTCATTTCTC 483 461 68 Antisense
GAAATCTTGCTCATTGTGTGCCTTC 216 363 100 Antisense
GTGTGCCTTCATTTCGCAGTTTAAA 585 487 115 Antisense
TATTTTTATCACTCTTATTTTCCAA 307 661 140 Antisense
ATCAAATCCTTTTTACCGAATCACA 395 27 167 Antisense
GAATCACAAATCGACGCGGTGGCCA 650 327 184 Antisense
GACGCGGTGGCCATATGTTTTAGGG 209 377 196 Antisense
TTAGGGTATTCTGATTTCCACGCTT 347 637 215 Antisense
GAAATCTGCTCTTGTTTGTTTTTTT 253 363 250 Antisense
TAATTTGTGTATAACGTTCTCGACC 77 633 292 Antisense
Sequence Source Affymetrix Proprietary Database