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[174946_at] p21 ras-related rho (RhoA)

Description CEK103G7R_rc /REP_DB=TREMBL Accession /GB=D72504 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk103g7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 57      6grp_total > 05      4grp_total > 61      6grp_cgc > 03      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2644
1 2 3 4 range
A 2770.6
(3)
P 		1678.9
(0.2)
P 		2480.3
(2.3)
P 		415.7
(-3)
P 		2355
B 3059.8
(3)
P 		2567.7
(1)
P 		2079.5
(-1)
P 		1603.8
(-3)
P 		1456
C 2784.5
(3)
P 		2522.8
(2)
P 		2168
(0.8)
P 		1136.6
(-3)
P 		1648
avr 2871.6
(3) 		2256.5
(1) 		2242.6
(0.9) 		1052
(-3) 		1820

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2131
1 2 3 4 5 6 range
A 2613.9
(1.8)
P 		2145
(-0.3)
P 		1521
(-3)
P 		1939.9
(-1.2)
P 		2882.8
(3)
P 		2444.4
(1.1)
P 		1362
B 2577.5
(-0.8)
P 		3177
(3)
P 		2229.1
(-3)
P 		2482
(-1.4)
P 		2301.5
(-2.5)
P 		2405.5
(-1.9)
P 		948
C 3387.2
(3)
P 		3080.7
(2.1)
P 		2294.5
(-0.1)
P 		2151.6
(-0.5)
P 		2347.6
(0.1)
P 		1256.3
(-3)
P 		2131
avr 2859.5
(3) 		2800.9
(2.6) 		2014.9
(-3) 		2191.2
(-1.7) 		2510.6
(0.5) 		2035.4
(-2.9) 		845
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 948
1 2 3 4 range
A 1008.8
(3)
P 		364.4
(-1.1)
P 		648.4
(0.7)
P 		60.9
(-3)
P 		948
B 975.7
(3)
P 		634.2
(-0.2)
P 		481.9
(-1.7)
P 		341.8
(-3)
P 		634
C 921.8
(3)
P 		725.3
(1.4)
P 		755.6
(1.6)
P 		191.7
(-3)
P 		730
avr 968.8
(3) 		574.6
(-0.1) 		628.6
(0.4) 		198.1
(-3) 		771

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 924
1 2 3 4 5 6 range
A 481.9
(-1.3)
P 		762.9
(1.8)
P 		331.2
(-3)
P 		492
(-1.2)
P 		869.9
(3)
P 		595.1
(-0.1)
P 		539
B 896.8
(1.1)
P 		1057.5
(3)
P 		541.3
(-3)
P 		657.4
(-1.7)
P 		935.1
(1.6)
P 		897
(1.1)
P 		516
C 1255.5
(3)
P 		940
(0.7)
P 		584
(-1.8)
P 		432.7
(-2.9)
P 		759.6
(-0.6)
P 		420.6
(-3)
P 		835
avr 878.1
(2.4) 		920.1
(3) 		485.5
(-3) 		527.4
(-2.4) 		854.9
(2.1) 		637.6
(-0.9) 		435
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 174946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.21268
Sequence Type Exemplar sequence
Representative Public ID CEK103G7R NCBI
Target Description CEK103G7R_rc /REP_DB=TREMBL Accession /GB=D72504 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk103g7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070558 NCBI Caenorhabditis elegans RHO (small G protein) family member (rho-1) (rho-1) mRNA, complete cds. 11/11 A 
Y51H4A.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:16482496:16488981:-1 gene:Y51H4A.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16482801-16483108(-) 87.3 100.0
Public Domain and Genome References
Gene Title p21 ras-related rho (RhoA)
Gene Symbol rho-1
Ensembl Y51H4A.3
Entrez Gene 178458 Entrez gene
SwissProt Q22038 EMBL-EBI
WormBase CE25369 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604207_AT ras homolog gene family, member A cfa
CANINE_2:CFA.20231.1.A1_S_AT ras homolog gene family, member A cfa
CANINE_2:CFA.20231.1.A2_AT ras homolog gene family, member A cfa
DROSOPHILA_2:1626808_S_AT glass multimer reporter construct of Hariharan dm
DROSOPHILA_2:1625488_S_AT glass multimer reporter construct of Hariharan dm
DROSGENOME1:152814_AT glass multimer reporter construct of Hariharan dm
CHICKEN:GGA.4963.1.S1_AT ras homolog gene family, member A gga
CHICKEN:GGA.4963.2.S1_A_AT ras homolog gene family, member A gga
CHICKEN:GGAAFFX.12357.1.S1_S_AT ras homolog gene family, member A gga
PLASMODIUM_ANOPHELES:200059_S_AT ras homolog gene family, member A hs
HG-U133B:200059_S_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:200059_S_AT ras homolog gene family, member A hs
HG-FOCUS:200059_S_AT ras homolog gene family, member A hs
HG-U133A_2:200059_S_AT ras homolog gene family, member A hs
HG-U133A:200059_S_AT ras homolog gene family, member A hs
U133_X3P:200059_S_AT ras homolog gene family, member A hs
HG-U95AV2:1394_AT ras homolog gene family, member A hs
HC-G110:1394_AT ras homolog gene family, member A hs
HG-U95AV2:37309_AT ras homolog gene family, member A hs
HUGENEFL:L25080_AT ras homolog gene family, member A hs
U133_X3P:HS2.77273.2.S1_3P_S_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:1555814_A_AT ras homolog gene family, member A hs
HG-U133_PLUS_2:240337_AT Ras homolog gene family, member A hs
HG-U133B:240337_AT Ras homolog gene family, member A hs
HG-U133_PLUS_2:241097_AT Ras homolog gene family, member A hs
HG-U133B:241097_AT Ras homolog gene family, member A hs
HG-U95E:69748_R_AT Ras homolog gene family, member A hs
U133_X3P:HS.201173.0.A1_3P_AT Ras homolog gene family, member A hs
MU11KSUBA:AA161790_S_AT ras homolog gene family, member A mm
MU11KSUBB:MSA.3406.0_S_AT ras homolog gene family, member A mm
MG-U74AV2:101112_G_AT ras homolog gene family, member A mm
MG-U74AV2:101113_AT ras homolog gene family, member A mm
MOE430A:1437628_S_AT ras homolog gene family, member A mm
MOUSE430A_2:1437628_S_AT ras homolog gene family, member A mm
MOUSE430_2:1437628_S_AT ras homolog gene family, member A mm
MG-U74AV2:101111_AT ras homolog gene family, member A mm
MG-U74CV2:134677_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBA:TC14290_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBA:TC24964_AT Ras homolog gene family, member A (Rhoa), mRNA mm
MU19KSUBC:TC39157_F_AT Ras homolog gene family, member A (Rhoa), mRNA mm
RG-U34A:D84477_AT ras homolog gene family, member A rn
RG-U34C:RC_AI179356_AT ras homolog gene family, member A rn
RG-U34C:RC_AA819664_AT ras homolog gene family, member A rn
RAE230A:1399027_AT ras homolog gene family, member A rn
RAT230_2:1399027_AT ras homolog gene family, member A rn
RAE230A:1370130_AT ras homolog gene family, member A rn
RAT230_2:1370130_AT ras homolog gene family, member A rn
RG-U34B:RC_AA964181_AT Ras homolog gene family, member A rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63379 Hypothetical protein Y51H4A.3 [Caenorhabditis elegans] ref|NP_502959.1| RHO (small G protein) family member (rho-1) [Caenorhabditis elegans] sp|Q22038|RHOA_CAEEL RAS-like GTP-binding protein RhoA gb|AAC37216.1| guanine nucleotide regulatory protein emb|CAE68045.1| Hypothetical protein CBG13664 [Caenorhabditis briggsae] 1.0E-97
blast AAY56741 guanine nucleotide regulatory protein [Caenorhabditis remanei] 3.0E-96
blast EAA44533 ENSANGP00000024640 [Anopheles gambiae str. PEST] gb|EAA09459.2| ENSANGP00000015684 [Anopheles gambiae str. PEST] ref|XP_314052.2| ENSANGP00000015684 [Anopheles gambiae str. PEST] ref|XP_314051.1| ENSANGP00000024640 [Anopheles gambiae str. PEST] 5.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.6E-94
Sequence
Target Sequence 
>C. ELEGANS:174946_AT
tcgattttaaggactcttggctccagagcttgctccagaaaaaaattatcgatttttctc
gattaatttaagaaatgcgtgcgatatttgtaacattttcctctctctttctctatttct
cttcgtctctccctctctccagcagcagcagaagctcccccccatacatctttaatggtt
ttttttattgaatgacagcaaactttccatccaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATTTTAAGGACTCTTGGCTCCA 609 601 65 Antisense
AAGGACTCTTGGCTCCAGAGCTTGC 353 161 73 Antisense
GGCTCCAGAGCTTGCTCCAGAAAAA 655 539 83 Antisense
CAGAGCTTGCTCCAGAAAAAAATTA 575 205 88 Antisense
TCGATTTTTCTCGATTAATTTAAGA 688 603 113 Antisense
GAAATGCGTGCGATATTTGTAACAT 65 363 136 Antisense
ATATTTGTAACATTTTCCTCTCTCT 398 19 148 Antisense
TCCCCCCCATACATCTTTAATGGTT 650 595 220 Antisense
ATGGTTTTTTTTATTGAATGACAGC 473 49 239 Antisense
GAATGACAGCAAACTTTCCATCCAA 337 331 254 Antisense
ATGACAGCAAACTTTCCATCCAATT 214 45 256 Antisense
Sequence Source Affymetrix Proprietary Database